Proteomic and functional characterization of the outer membrane vesicles from the gastric pathogen Helicobacter pylori

2009 ◽  
Vol 3 (7) ◽  
pp. 785-796 ◽  
Author(s):  
Erica Mullaney ◽  
Paul A. Brown ◽  
Sinead M. Smith ◽  
Catherine H. Botting ◽  
Yoshio Y. Yamaoka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Functional Characterization ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Gastric Pathogen

scholarly journals Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori

2019 ◽  
Vol 34 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Maurizio Ronci ◽  
Sonia Del Prete ◽  
Valentina Puca ◽  
Simone Carradori ◽  
Vincenzo Carginale ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Identification And Characterization

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

scholarly journals Helicobacter pylori Outer Membrane Vesicles and Extracellular Vesicles from Helicobacter pylori-Infected Cells in Gastric Disease Development

2021 ◽  
Vol 22 (9) ◽  
pp. 4823
Author(s):  
María Fernanda González ◽  
Paula Díaz ◽  
Alejandra Sandoval-Bórquez ◽  
Daniela Herrera ◽  
Andrew F. G. Quest
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Extracellular Vesicles ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Host Cells ◽  
Gastric Epithelium ◽  
Infected Cells ◽  
H Pylori

Extracellular vesicles (EVs) are cell-derived vesicles important in intercellular communication that play an essential role in host-pathogen interactions, spreading pathogen-derived as well as host-derived molecules during infection. Pathogens can induce changes in the composition of EVs derived from the infected cells and use them to manipulate their microenvironment and, for instance, modulate innate and adaptive inflammatory immune responses, both in a stimulatory or suppressive manner. Gastric cancer is one of the leading causes of cancer-related deaths worldwide and infection with Helicobacter pylori (H. pylori) is considered the main risk factor for developing this disease, which is characterized by a strong inflammatory component. EVs released by host cells infected with H. pylori contribute significantly to inflammation, and in doing so promote the development of disease. Additionally, H. pylori liberates vesicles, called outer membrane vesicles (H. pylori-OMVs), which contribute to atrophia and cell transformation in the gastric epithelium. In this review, the participation of both EVs from cells infected with H. pylori and H. pylori-OMVs associated with the development of gastric cancer will be discussed. By deciphering which functions of these external vesicles during H. pylori infection benefit the host or the pathogen, novel treatment strategies may become available to prevent disease.

scholarly journals Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst

2018 ◽  
Vol 9 ◽  
Author(s):  
Sujinna Lekmeechai ◽  
Yu-Ching Su ◽  
Marta Brant ◽  
Maria Alvarado-Kristensson ◽  
Anna Vallström ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Respiratory Burst ◽  
Reactive Oxygen ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Oxygen Species

Lewis Epitopes on Outer Membrane Vesicles of Relevance to Helicobacter pylori Pathogenesis

Helicobacter ◽  
2005 ◽  
Vol 10 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Sean O. Hynes ◽  
Jacqueline I. Keenan ◽  
John A. Ferris ◽  
Heidi Annuk ◽  
Anthony P. Moran
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

Characterization of outer membrane vesicles of Acetobacter pasteurianus NBRC3283

2018 ◽  
Vol 125 (4) ◽  
pp. 425-431 ◽  
Author(s):  
Masahito Hashimoto ◽  
Taichi Matsumoto ◽  
Miwa Tamura-Nakano ◽  
Mami Ozono ◽  
Shuhei Hashiguchi ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Acetobacter Pasteurianus

scholarly journals Helicobacter pylori outer membrane vesicles involvement in the infection development and Helicobacter pylori-related diseases

2018 ◽  
Vol 25 (1) ◽  
Author(s):  
Magdalena Chmiela ◽  
Natalia Walczak ◽  
Karolina Rudnicka
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles
Sign in / Sign up

Export Citation Format

Share Document