Polarity of prostate specific membrane antigen, prostate stem cell antigen, and prostate specific antigen in prostate tissue and in a cultured epithelial cell line

The Prostate ◽  
2003 ◽  
Vol 55 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Jason J. Christiansen ◽  
Sigrid A. Rajasekaran ◽  
Peggy Moy ◽  
Anthony Butch ◽  
Lee Goodglick ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cell ◽  
Cell Line ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Epithelial Cell Line ◽  
Prostate Specific Membrane Antigen ◽  
Prostate Stem Cell Antigen ◽  
Cell Antigen ◽  
Specific Membrane

scholarly journals Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line

1996 ◽  
Vol 17 (8) ◽  
pp. 1641-1646 ◽  
Author(s):  
Mukta M. Webber ◽  
Diana Bello ◽  
Hynda K. Kleinman ◽  
David D. Wartinger ◽  
Daniel E. Williams ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Epithelial Cell ◽  
Cell Line ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Epithelial Cell Line ◽  
Adult Human ◽  
Prostatic Epithelial Cell

scholarly journals Expression of prostate-specific antigen and prostate-specific membrane antigen transcripts in blood cells: implications for the detection of hematogenous prostate cells and standardization

1998 ◽  
Vol 44 (3) ◽  
pp. 472-481 ◽  
Author(s):  
Jean-Luc Gala ◽  
Michel Heusterspreute ◽  
Sylvain Loric ◽  
France Hanon ◽  
Bertrand Tombal ◽  
...  
Keyword(s):  
Cell Line ◽  
Prostate Specific Antigen ◽  
Cell Lines ◽  
Specific Antigen ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Blood Samples ◽  
Prostate Cells ◽  
Specific Membrane

Abstract Circulating prostate cells can be detected in cancer patients by using reverse transcriptase–PCR (RT-PCR) assay for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) mRNA. A quality-control study involving a conventional RT-PCR assay was performed and, surprisingly, detected both transcripts in many negative control cell lines and in normal blood samples. The existence of an illegitimate transcription of the PSA and PSM genes was evidenced by sequence analysis of several PSM and PSA-PCR products. Sequencing indeed demonstrated the presence of a PSA or PSM polymorphism in some but not all the cell lines and patient samples, as well as a heterozygous mutation (G to A; Asp to Asn) in the Jurkat cell line. Moreover, the amount of PSA transcript in MCF-7, a PSA-negative breast line, increased after incubation with cycloheximide. Interestingly, the frequency of positivity was as high as 12% in male samples if only tested once, but dropped to 3% upon multiple testing of the same cDNA. This highlights the stochastic effects in RT-PCR results at high sensitivity, hence the importance of repetitive testing in clinical samples. Decreasing the number of cycles avoided the amplification of illegitimate transcripts but also affected the limit of detection, as evidenced with PSA and PSM cDNA containing plasmids, mixing of LNCap with normal blood samples, and the PSA-PSM-negative K562 cell line. The current data raise the need for a multicentric standardization of the RT-PCR methodology used to amplify PSA and PSM transcripts.

Simultaneous targeting of prostate stem cell antigen and prostate-specific membrane antigen improves the killing of prostate cancer cells using a novel modular T cell-retargeting system

The Prostate ◽  
2014 ◽  
Vol 74 (13) ◽  
pp. 1335-1346 ◽  
Author(s):  
Claudia Arndt ◽  
Anja Feldmann ◽  
Stefanie Koristka ◽  
Marc Cartellieri ◽  
Maria Dimmel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cell ◽  
T Cell ◽  
Cancer Cells ◽  
Prostate Specific Membrane Antigen ◽  
Prostate Stem Cell Antigen ◽  
Prostate Cancer Cells ◽  
Cell Antigen ◽  
Specific Membrane

Can 68Ga-PSMA PET/CT-derived prostate-specific membrane antigen expression parameters predict prostate-specific antigen response to enzalutamide treatment?

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Savaş Karyağar ◽  
Osman Güven ◽  
Sevda Sağlampinar Karyağar ◽  
Serdar Arici ◽  
Oğuzhan Selvi ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Expression ◽  
Prostate Specific Membrane Antigen ◽  
Psma Pet ◽  
Pet Ct ◽  
Specific Membrane

scholarly journals Androgen induces differentiation of a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line

2001 ◽  
Vol 170 (1) ◽  
pp. 287-296 ◽  
Author(s):  
MT Ling ◽  
KW Chan ◽  
CK Choo
Keyword(s):  
Human Papillomavirus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Specific Antigen ◽  
Telomerase Activity ◽  
Epithelial Cell Line ◽  
Prostate Cell ◽  
Human Papillomavirus 16 ◽  
Prostate Epithelial Cell ◽  
Prostate Epithelial Cells

Androgen signaling is crucial for the growth and development, as well as for tumorigenesis of the prostate. However, many of the prostate epithelial cell lines developed previously, either normal or tumorigenic, do not express androgen receptor (AR) or respond to androgen. In order to advance our understanding on how androgen signaling regulates the growth and the differentiation status, and affects tumorigenicity of the epithelial cell, we performed experiments on HPr-1, a prostate cell line recently immortalized from normal human prostate epithelial cells. In the present study, AR was stably transfected into HPr-1 cells by replication-defective retrovirus. Treatment of HPr-1AR cells with androgen resulted in cell differentiation and growth retardation accompanied with up-regulation of cytokeratins K8 and K18, prostate specific antigen, p21 and p27, and down-regulation of c-myc, bcl-2 and telomerase activity. Our results suggest that androgen promotes the process of differentiation in a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line which may reflect the normal effects of androgen on prostate cells.

Duplex RT-PCR and chemiluminometric hybridization assay for combined screening of the mRNAs of prostate-specific antigen and prostate-specific membrane antigen in peripheral blood

2005 ◽  
Vol 531 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Evaggelia Emmanouilidou ◽  
Bakhos Tannous ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Prostate Specific Antigen ◽  
Peripheral Blood ◽  
Specific Antigen ◽  
Hybridization Assay ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Specific Membrane

scholarly journals A Human Corneal Epithelial Cell Line Model for Limbal Stem Cell Biology and Limbal Immunobiology

2016 ◽  
Vol 6 (3) ◽  
pp. 761-766 ◽  
Author(s):  
Bakiah Shaharuddin ◽  
Sajjad Ahmad ◽  
Nani Md Latar ◽  
Simi Ali ◽  
Annette Meeson
Keyword(s):  
Stem Cell ◽  
Epithelial Cell ◽  
Cell Line ◽  
Cell Biology ◽  
Corneal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Stem Cell Biology ◽  
Corneal Epithelial ◽  
Cell Line Model ◽  
Limbal Stem Cell
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