Gene Modification and Three‐Dimensional Scaffolds as Novel Tools to Allow the Use of Postnatal Thymic Epithelial Cells for Thymus Regeneration Approaches

Thymocytes depend on the interaction with thymic epithelial cells for the generation of a diverse, nonautoreactive T cell repertoire. In turn, thymic epithelial cells acquire their three-dimensional cellular organization via instructive signals from developing thymocytes. The nature of these signals has been elusive so far. We show that thymocytes and medullary epithelial cells (MECs) communicate via the lymphotoxin β receptor (LTβR) signaling axis. Normal differentiation of thymic MECs requires LTβR ligand on thymocytes and LTβR together with nuclear factor–κB-inducing kinase (Nik) in thymic epithelial cells. Impaired lympho–epithelial cross talk in the absence of the LTβR causes aberrant differentiation and reduced numbers of thymic MECs, leads to the retention of mature T lymphocytes, and is associated with autoimmune phenomena, suggesting an unexpected role for LTβR signaling in central tolerance induction.

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Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution

Regulatory Peptides ◽

10.1016/j.regpep.2006.12.015 ◽

2007 ◽

Vol 141 (1-3) ◽

pp. 86-95 ◽

Cited By ~ 13

Author(s):

Hee-Woo Lee ◽

Sung-Min Kim ◽

Na-Ri Shim ◽

Soo-Kyung Bae ◽

Il-Gun Jung ◽

...

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Epithelial Cells ◽

Thymic Epithelial Cells ◽

Thymic Involution ◽

Nerve Growth ◽

Thymus Regeneration

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Thymic Epithelial Cells Induced from Pluripotent Stem Cells by a Three-Dimensional Spheroid Culture System Regenerates Functional T Cells in Nude Mice

Cellular Reprogramming ◽

10.1089/cell.2015.0006 ◽

2015 ◽

Vol 17 (5) ◽

pp. 368-375 ◽

Cited By ~ 7

Author(s):

Motohito Okabe ◽

Sachiko Ito ◽

Naomi Nishio ◽

Yuriko Tanaka ◽

Ken-Ichi Isobe

Keyword(s):

Stem Cells ◽

T Cells ◽

Epithelial Cells ◽

Nude Mice ◽

Pluripotent Stem Cells ◽

Culture System ◽

Three Dimensional ◽

Thymic Epithelial Cells ◽

Spheroid Culture

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Altered thymic epithelial cells may be decisive for Moloney virus-induced lymphoma development

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077499 ◽

1983 ◽

Vol 41 ◽

pp. 784-785

Author(s):

U.I. Heine ◽

G.R.F. Krueger ◽

E. Munoz ◽

A. Karpinski

Keyword(s):

Epithelial Cells ◽

Leukemia Virus ◽

Light And Electron Microscopy ◽

Tumor Development ◽

Current Evidence ◽

Thymic Epithelial Cells ◽

Thymic Tissue ◽

Newborn Mice ◽

Moloney Leukemia Virus ◽

Differentiation Block

Infection of newborn mice with Moloney leukemia virus (M-MuLV) causes a T-cell differentiation block in the thymic cortex accompanied by proliferation and accumulation of prethymic lymphoblasts in the thymus and subsequent spreading of these cells to generate systemic lymphoma. Current evidence shows that thymic reticular epithelial cells (REC) provide a microenvironment necessary for the maturation of prethymic lymphoblasts to mature T-lymphocytes by secretion of various thymic factors. A change in that environment due to infection of REC by virus could be decisive for the failure of lymphoblasts to mature and thus contribute to lymphoma development.We have studied the morphology and distribution of the major thymic cell populations at different stages of tumorigenesis in Balb/c mice infected when newborn with 0.2ml M-MuLV suspension, 6.8 log FFU/ml. Thymic tissue taken at 1-2 weekly intervals up to tumor development was processed for light and electron microscopy, using glutaraldehyde-OsO4fixation and Epon-Araldite embedding.

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Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123283 ◽

1992 ◽

Vol 50 (1) ◽

pp. 576-577

Author(s):

Julian P. Heath ◽

Buford L. Nichols ◽

László G. Kömüves

Keyword(s):

Electron Microscopy ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Three Dimensional ◽

Intestinal Epithelial ◽

Cell Surfaces ◽

Basal Surface ◽

Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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