scholarly journals First report on the molecular detection of Entamoeba bovis from the endangered wild water buffalo ( Bubalus arnee ) in Nepal

2021 ◽  
Author(s):  
Menuka Aryal ◽  
Roshan Babu Adhikari ◽  
Prakriti Kandel ◽  
Tirth Raj Ghimire ◽  
Deegendra Khadka ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Water Buffalo ◽  
First Report

First report on molecular detection and identification of Tomato aspermy virus naturally occurring on gladiolus in India

2011 ◽  
Vol 39 (3) ◽  
pp. 303-307 ◽  
Author(s):  
S. K. Raj ◽  
S. Kumar ◽  
D. K. Verma ◽  
S. K. Snehi
Keyword(s):  
Molecular Detection ◽  
First Report ◽  
Tomato Aspermy Virus ◽  
Naturally Occurring ◽  
Detection And Identification

scholarly journals Microscopic and Molecular Detection of Camel Piroplasmosis in Gadarif State, Sudan

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Abdalla Mohamed Ibrahim ◽  
Ahmed A. H. Kadle ◽  
Hamisi Said Nyingilili
Keyword(s):  
Molecular Detection ◽  
Blood Film ◽  
The State ◽  
Economic Consequences ◽  
Camelus Dromedarius ◽  
Molecular Technique ◽  
Eastern Region ◽  
Prevalence Rates ◽  
First Report ◽  
Higher Sensitivity

The socioeconomic importance of camels (Camelus dromedarius) could not be neglected in the Sudan. The present study was planned to confirm the presence of piroplasmosis in camels from the Eastern region of the Sudan (Gedarif State) using microscopical (blood film) and molecular technique (PCR). A total of 55 camels of different sexes (34 females and 21 males) were sampled from four localities of the state between January 2011 and January 2012. The prevalence rates using parasitological and molecular examinations were 43.6% and 74.5%, respectively. The prevalence rates significantly vary between the localities (p=0.011) but not between the different sexes (p=0.515). PCR technique showed higher sensitivity than microscopy. The present paper was to be the first report investigating camel piroplasmosis using both parasitological and molecular methods in the Eastern region of the Sudan. Further studies in the phylogenetic sequencing are to be continued for parasite speciation. Moreover, studies on the clinical and economic consequences of camel piroplasmosis are recommended.

First Report on Molecular Detection of Equine Upper Respiratory Infectious Viruses in Republic of Korea

2013 ◽  
Vol 33 (8) ◽  
pp. 628-636 ◽  
Author(s):  
Sungjin Ko ◽  
Jun-Gu Kang ◽  
Jung-Yong Yeh ◽  
Jin-San Moon ◽  
Gui-Cheol Choi ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Republic Of Korea ◽  
First Report ◽  
Upper Respiratory

scholarly journals The first report of the prevalence of Nosema ceranae in Bulgaria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4252 ◽  
Author(s):  
Rositsa Shumkova ◽  
Ani Georgieva ◽  
Georgi Radoslavov ◽  
Daniela Sirakova ◽  
Gyulnas Dzhebir ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Molecular Detection ◽  
Ssu Rdna ◽  
Nosema Ceranae ◽  
Nosema Apis ◽  
Pcr Assay ◽  
First Report ◽  
Duplex Pcr Assay ◽  
Different Parts

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

scholarly journals Molecular Detection of Lentogenic Strain of Newcastle Disease Virus in Commercial Broiler Chickens Using Sentinel Birds in Iran: The First Report

2015 ◽  
Vol 9 (3) ◽  
pp. 32-36
Author(s):  
Hossein Hosseini ◽  
Amir Gholami ◽  
Arash Ghalyanchi Langeroudi ◽  
◽  
◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Molecular Detection ◽  
First Report ◽  
Commercial Broiler

scholarly journals The first report of hydatid disease (Echinococcus granulosus) in an Australian water buffalo (Bubalus bubalis)

2019 ◽  
Vol 8 ◽  
pp. 256-259 ◽  
Author(s):  
D.J. Jenkins ◽  
T. Williams ◽  
S. Raidal ◽  
C. Gauci ◽  
M.W. Lightowlers
Keyword(s):  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Australian Water ◽  
First Report

First Report on the Frequency and Molecular Detection of Neuropathogenic EHV-1 in Turkey

2012 ◽  
Vol 32 (9) ◽  
pp. 525-530 ◽  
Author(s):  
Huseyin Yilmaz ◽  
Eda Altan ◽  
Nuri Turan ◽  
Aydin Gurel ◽  
Damla Haktanir ◽  
...  
Keyword(s):  
Molecular Detection ◽  
First Report

scholarly journals Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea

2020 ◽  
Vol 58 (3) ◽  
pp. 327-331
Author(s):  
Ju Yeong Kim ◽  
You Shine Kwak ◽  
In-Yong Lee ◽  
Tai-Soon Yong
Keyword(s):  
Toxoplasma Gondii ◽  
Neurological Disease ◽  
Tick Species ◽  
Molecular Detection ◽  
Development Stage ◽  
Haemaphysalis Longicornis ◽  
First Report ◽  
B1 Gene ◽  
Haemaphysalis Flava ◽  
Human Adults

Toxoplasma gondii are intracellular protozoa that can cause neurological disease or death in fetuses and even in immunocompromised human adults. Ticks are recognized as vectors of many microorganisms including viruses, bacteria, and protozoa. Recent studies detected T. gondii in various tick species in many countries. In this study, we performed PCR detection of the T. gondii B1 gene from Haemaphysalis ticks collected from vegetation in 4 localities, Wonju, Gunsan, Miryang, and Yangsan, in Korea. We analyzed DNA from 314 ticks (268 Haemaphysalis longicornis and 46 Haemaphysalis flava) and the B1 gene of T. gondii was detected in 13 of these. The detection of T. gondii in ticks differed significantly by region (P=0.021). T. gondii was detected in the following percentages of collected ticks: 3.7% (7 of 189) in Gunsan, 10% (5 of 50) in Wonju, 16.7% (1 of 6) in Yangsan, and 0% (0 of 69) in Miryang. The detection of T. gondii in ticks was not associated with tick species or development stage. This is the first report of T. gondii detection in ticks in Korea. Our results provide important information necessary to understand toxoplasmosis transmission.

scholarly journals Molecular detection of Rickettsia genus in chigger mites (Trombidiformes: Trombiculidae) collected on small mammals in southeastern brazilian

2019 ◽  
Vol 28 (4) ◽  
pp. 563-568
Author(s):  
Fernando de Castro Jacinavicius ◽  
Ricardo Bassini-Silva ◽  
Sebastián Muñoz-Leal ◽  
Cal Welbourn ◽  
Ronald Ochoa ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Small Mammals ◽  
18S Rdna ◽  
Molecular Detection ◽  
Molecular Analyses ◽  
First Report ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
First Time

Abstract Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to “Candidatus Rickettsia colombianensi”, a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.

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