Quantification of gold nanoparticles in histologically thin tissue slices using TXRF

2021 ◽  
Author(s):  
Gabriella Mankovskii ◽  
Ana Pejović‐Milić
Keyword(s):  
Gold Nanoparticles ◽  
Tissue Slices

Automated 3-D cell population analysis in thick tissue sections using laser-scanning confocal-microscopy data

1993 ◽  
Vol 51 ◽  
pp. 562-563
Author(s):  
Hakan Ancin
Keyword(s):  
Laser Scanning ◽  
Population Analysis ◽  
Three Dimensional ◽  
Large Data ◽  
Laser Scanning Confocal Microscopy ◽  
Tissue Slices ◽  
Scanning Confocal Microscopy ◽  
Tissue Sections ◽  
Spatially Adaptive ◽  
Microscopy Data

This paper presents methods for performing detailed quantitative automated three dimensional (3-D) analysis of cell populations in thick tissue sections while preserving the relative 3-D locations of cells. Specifically, the method disambiguates overlapping clusters of cells, and accurately measures the volume, 3-D location, and shape parameters for each cell. Finally, the entire population of cells is analyzed to detect patterns and groupings with respect to various combinations of cell properties. All of the above is accomplished with zero subjective bias.In this method, a laser-scanning confocal light microscope (LSCM) is used to collect optical sections through the entire thickness (100 - 500μm) of fluorescently-labelled tissue slices. The acquired stack of optical slices is first subjected to axial deblurring using the expectation maximization (EM) algorithm. The resulting isotropic 3-D image is segmented using a spatially-adaptive Poisson based image segmentation algorithm with region-dependent smoothing parameters. Extracting the voxels that were labelled as "foreground" into an active voxel data structure results in a large data reduction.

EpCam-specific Gold Nanoparticles for Detection, Live-imaging and Rapid Enrichment of Adult Human Liver Progenitor Cells

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
N Fekete-Drimusz ◽  
J de la Roche ◽  
F Vondran ◽  
CL Sajti ◽  
MP Manns ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Progenitor Cells ◽  
Human Liver ◽  
Live Imaging ◽  
Adult Human

Enhanced radiation efficiency by gold nanoparticles after proton beam irradiation in medulloblastoma cell lines

2016 ◽  
Vol 228 (06/07) ◽  
Author(s):  
A Rashidi ◽  
S Barcikovski ◽  
S Jendrzej ◽  
S Tippelt ◽  
G Fleischhack ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Proton Beam ◽  
Cell Lines ◽  
Radiation Efficiency ◽  
Beam Irradiation ◽  
Proton Beam Irradiation ◽  
Medulloblastoma Cell ◽  
Medulloblastoma Cell Lines

Apoptosis Investigation in Liver Metastases with Precision Cut Tissue Slices (PCTS)

2005 ◽  
Vol 43 (01) ◽  
Author(s):  
HU Kasper ◽  
E Konze ◽  
D Stippel ◽  
U Drebber ◽  
HP Dienes
Keyword(s):  
Liver Metastases ◽  
Tissue Slices
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