Endogenous NADPH-dependent aldose reductase activity influences product formation during xylose consumption in recombinantSaccharomyces cerevisiae

Yeast ◽  
2004 ◽  
Vol 21 (2) ◽  
pp. 141-150 ◽  
Author(s):  
K. L. Träff-Bjerre ◽  
M. Jeppsson ◽  
B. Hahn-Hägerdal ◽  
M.-F. Gorwa-Grauslund
Keyword(s):  
Aldose Reductase ◽  
Reductase Activity ◽  
Product Formation ◽  
Xylose Consumption

DW1029M, a novel botanical drug candidate, inhibits advanced glycation end-product formation, rat lens aldose reductase activity, and TGF-β1 signaling

2014 ◽  
Vol 306 (10) ◽  
pp. F1161-F1170 ◽  
Author(s):  
Joobyoung Yoon ◽  
Hyunyong Lee ◽  
Hwan Bong Chang ◽  
Hyunsik Choi ◽  
Yong Sung Kim ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Aldose Reductase ◽  
Transforming Growth Factor ◽  
Reductase Activity ◽  
Product Formation ◽  
Drug Candidate ◽  
Advanced Glycation ◽  
Active Constituents ◽  
Rat Lens Aldose Reductase ◽  
Botanical Extract

DW1029M is a botanical extract consisting of Morus bark and Puerariae radix, produced by Dong-Wha Pharmaceutical, for nephroprotective drug development; it has been in phase II clinical trials in Korea. In our mechanistic investigations, we found that DW1029M inhibits advanced glycation end products (AGEs), rat lens aldose reductase (RLAR), and transforming growth factor (TGF)-β1 signaling, all of which are implicated in diabetic complications such as diabetic nephropathy and diabetic retinopathy. DW1029M inhibits AGE formation via Fe2+ chelation. The extract contains 13 active constituents that inhibit AGE formation, 8 active constituents that inhibit RLAR activity, and 1 inhibitor of TGF-β1 signaling. Our results suggest DW1029M protects against diabetic nephropathy via blockade of AGE formation, RLAR activity, and TGF-β1 signaling.

scholarly journals Dynamic Co-Cultivation Process of Corynebacterium glutamicum Strains for the Fermentative Production of Riboflavin

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Fernando Pérez-García ◽  
Arthur Burgardt ◽  
Dina R. Kallman ◽  
Volker F. Wendisch ◽  
Nadav Bar
Keyword(s):  
Corynebacterium Glutamicum ◽  
Xanthomonas Campestris ◽  
Product Formation ◽  
Volumetric Productivity ◽  
Riboflavin Production ◽  
Xylose Consumption ◽  
Single Strain ◽  
Sugar Mixtures ◽  
Fed Batch Fermentation ◽  
Overexpressing Strain

Residual streams from lignocellulosic processes contain sugar mixtures of glucose, xylose, and mannose. Here, the industrial workhorse Corynebacterium glutamicum was explored as a research platform for the rational utilization of a multiple sugar substrate. The endogenous manA gene was overexpressed to enhance mannose utilization. The overexpression of the xylA gene from Xanthomonas campestris in combination with the endogenous xylB gene enabled xylose consumption by C. glutamicum. Furthermore, riboflavin production was triggered by overexpressing the sigH gene from C. glutamicum. The resulting strains were studied during batch fermentations in flasks and 2 L lab-scale bioreactors separately using glucose, mannose, xylose, and a mixture of these three sugars as a carbon source. The production of riboflavin and consumption of sugars were improved during fed-batch fermentation thanks to a dynamic inoculation strategy of manA overexpressing strain and xylAB overexpressing strain. The final riboflavin titer, yield, and volumetric productivity from the sugar mixture were 27 mg L−1, 0.52 mg g−1, and 0.25 mg L−1 h−1, respectively. It reached a 56% higher volumetric productivity with 45% less by-product formation compared with an equivalent process inoculated with a single strain overexpressing the genes xylAB and manA combined. The results indicate the advantages of dynamic multi strains processes for the conversion of sugar mixtures.

Determination of AL01576 Concentration in Rat Lenses and Plasma by Bioassay for Aldose Reductase Activity Measurements

1989 ◽  
Vol 21 (4) ◽  
pp. 285-291 ◽  
Author(s):  
Otto Hockwin ◽  
Peter Müller ◽  
Jan Krolczyk ◽  
Bette A. McCue ◽  
Philip R. Mayer
Keyword(s):  
Aldose Reductase ◽  
Reductase Activity ◽  
Activity Measurements

scholarly journals Reverse Docking, Molecular Docking, Absorption, Distribution, and Toxicity Prediction of Artemisinin as an Anti-diabetic Candidate

Molekul ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 88
Author(s):  
Ruswanto Ruswanto ◽  
Richa Mardianingrum ◽  
Siswandono Siswandono ◽  
Dini Kesuma
Keyword(s):  
Molecular Docking ◽  
Aldose Reductase ◽  
Glucose Concentration ◽  
In Silico ◽  
Reductase Activity ◽  
Polyol Pathway ◽  
Toxicity Prediction ◽  
In Silico Studies

Aldose reductase is an enzyme that catalyzes one of the steps in the sorbitol (polyol) pathway that is responsible for fructose formation from glucose. In diabetes, aldose reductase activity increases as the glucose concentration increases. The purpose of this research was to identify and develop the use of artemisinin as an anti-diabetic candidate through in silico studies, including reverse docking, receptor analysis, molecular docking, drug scan, absorption, and distributions and toxicity prediction of artemisinin. Based on the results, we conclude that artemisinin can be used as an anti-diabetic candidate through inhibition of aldose reductase

Ginsenoside Rb1 alleviates diabetic kidney podocyte injury by inhibiting aldose reductase activity

2021 ◽  
Author(s):  
Jia-yi He ◽  
Quan Hong ◽  
Bi-xia Chen ◽  
Shao-yuan Cui ◽  
Ran Liu ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Reductase Activity ◽  
Ginsenoside Rb1 ◽  
Podocyte Injury ◽  
Diabetic Kidney
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