Different response to acetic acid stress inSaccharomyces cerevisiaewild-type andl-ascorbic acid-producing strains

Yeast ◽  
2013 ◽  
Vol 30 (9) ◽  
pp. 365-378 ◽  
Author(s):  
Francesca Martani ◽  
Tiziana Fossati ◽  
Riccardo Posteri ◽  
Lorenzo Signori ◽  
Danilo Porro ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Acid Stress ◽  
Different Response

scholarly journals Effect of Ascorbic Acid and Indolyl Acetic Acid on Regeneration of Willow Branches and Germination

Nature ◽  
1936 ◽  
Vol 137 (3467) ◽  
pp. 618-618 ◽  
Author(s):  
W. DAVIES ◽  
G. A. ATKINS ◽  
P. C. B. HUDSON
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Indolyl Acetic Acid

scholarly journals Sphingolipid biosynthesis upregulation by TOR complex 2–Ypk1 signaling during yeast adaptive response to acetic acid stress

2016 ◽  
Vol 473 (23) ◽  
pp. 4311-4325 ◽  
Author(s):  
Joana F. Guerreiro ◽  
Alexander Muir ◽  
Subramaniam Ramachandran ◽  
Jeremy Thorner ◽  
Isabel Sá-Correia
Keyword(s):  
Acetic Acid ◽  
Protein Kinase ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Industrial Biotechnology ◽  
Acetic Acid Tolerance ◽  
Spoilage Yeasts ◽  
Sphingolipid Biosynthesis

Acetic acid-induced inhibition of yeast growth and metabolism limits the productivity of industrial fermentation processes, especially when lignocellulosic hydrolysates are used as feedstock in industrial biotechnology. Tolerance to acetic acid of food spoilage yeasts is also a problem in the preservation of acidic foods and beverages. Thus understanding the molecular mechanisms underlying adaptation and tolerance to acetic acid stress is increasingly important in industrial biotechnology and the food industry. Prior genetic screens for Saccharomyces cerevisiae mutants with increased sensitivity to acetic acid identified loss-of-function mutations in the YPK1 gene, which encodes a protein kinase activated by the target of rapamycin (TOR) complex 2 (TORC2). We show in the present study by several independent criteria that TORC2–Ypk1 signaling is stimulated in response to acetic acid stress. Moreover, we demonstrate that TORC2-mediated Ypk1 phosphorylation and activation is necessary for acetic acid tolerance, and occurs independently of Hrk1, a protein kinase previously implicated in the cellular response to acetic acid. In addition, we show that TORC2–Ypk1-mediated activation of l-serine:palmitoyl-CoA acyltransferase, the enzyme complex that catalyzes the first committed step of sphingolipid biosynthesis, is required for acetic acid tolerance. Furthermore, analysis of the sphingolipid pathway using inhibitors and mutants indicates that it is production of certain complex sphingolipids that contributes to conferring acetic acid tolerance. Consistent with that conclusion, promoting sphingolipid synthesis by adding exogenous long-chain base precursor phytosphingosine to the growth medium enhanced acetic acid tolerance. Thus appropriate modulation of the TORC2–Ypk1–sphingolipid axis in industrial yeast strains may have utility in improving fermentations of acetic acid-containing feedstocks.

scholarly journals Endogenous lycopene improves ethanol production under acetic acid stress in Saccharomyces cerevisiae

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Shuo Pan ◽  
Bin Jia ◽  
Hong Liu ◽  
Zhen Wang ◽  
Meng-Zhe Chai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Ethanol Production ◽  
Acid Stress

scholarly journals Membrane Permeabilities of Ascorbic Acid and Ascorbate

Biomolecules ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 73 ◽  
Author(s):  
Christof Hannesschlaeger ◽  
Peter Pohl
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Lipid Bilayers ◽  
Redox Balance ◽  
The Other ◽  
Lipid Matrix ◽  
Transport Pathway ◽  
Transcellular Transport ◽  
Membrane Permeabilities ◽  
Ph Microelectrodes

Vitamin C (VC)—a collective term for the different oxidation and protonation forms of ascorbic acid (AscH)—is an essential micronutrient that serves as (i) a potent antioxidant and (ii) a cofactor of a manifold of enzymatic processes. Its role in health is related to redox balance maintenance, which is altered in diseases such as obesity, cancer, neurodegenerative diseases, hypertension, and autoimmune diseases. Despite its importance, VC uptake has been poorly investigated. Available literature values for the passive membrane permeability P of lipid bilayers for AscH scatter by about 10 orders of magnitude. Here, we show by voltage clamp that P − of AscH’s anionic form (ascorbate Asc − ) is negligible. To cross the membrane, Asc − picks up a proton in the membrane vicinity and releases it on the other side of the membrane. This leads to a near-membrane pH drop that was visualized by scanning pH microelectrodes. The AscH concentration dependent pH profiles indicated P   =   1.1   ±   0.1   ×   10 − 8   cm / s . Thus, AscH’s P is comparable to that of sorbitol and much lower than that of other weak acids like acetic acid or salicylic acid. The observation suggests that the capacity of the passive transcellular transport pathway across the lipid matrix does not suffice to ensure the required VC intake from the gastrointestinal tract.

INFLUENCE OF CHITOSAN SOLUTION ON THE VIABILITY OF ARBUSCULAR MYCORRHIZAL SPORES

2021 ◽  
pp. 7-9
Author(s):  
Nalini Singh ◽  
Sunita Chahar
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Chemical Method ◽  
Arbuscular Mycorrhizal ◽  
Chitosan Solution ◽  
Spore Viability ◽  
Tetrazolium Bromide ◽  
Shrimp Shells ◽  
Arbuscular Mycorrhizal Fungal

The study was aimed to fortify the Arbuscular Mycorrhizal Fungal (AMF) spores, widely used in Bio-fertilizers with chitosan and check the inuence of chitosan on the viability of AMF spores. Chitosan was prepared from shrimp shells using chemical method involving demineralization, deproteinization and deacetylation.AMF spores were kept in three different concentrations of chitosan (0.1%, 0.5 %and 1%) which was prepared in 0.1% acetic acid and 0.1% ascorbic acid. Spore viability was checked by the MTT 3-(4, 5 th th th dimethylthiazol-yl-2, 5-diphenyl-2H-tetrazolium bromide) after a day, 10 day, 20 day and 30 day. Highest number of viable spores was observed in chitosan dissolved in 0.1% ascorbic acid as compared to chitosan in acetic acid.

Transcriptome response of Acetobacter pasteurianus Ab3 to high acetic acid stress during vinegar production

2020 ◽  
Vol 104 (24) ◽  
pp. 10585-10599
Author(s):  
Kai Xia ◽  
Chengcheng Han ◽  
Jun Xu ◽  
Xinle Liang
Keyword(s):  
Acetic Acid ◽  
Acid Stress ◽  
Acetobacter Pasteurianus ◽  
Transcriptome Response
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