Metabolic changes of Issatchenkia orientalis under acetic acid stress by transcriptome profile using RNA-sequencing

2021 ◽  
Author(s):  
Yueqi Li ◽  
Yingdi Li ◽  
Ruoyun Li ◽  
Lianliang Liu ◽  
Yingjie Miao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Rna Sequencing ◽  
Acid Stress ◽  
Metabolic Changes ◽  
Transcriptome Profile ◽  
Issatchenkia Orientalis

Integrated transcriptomic and proteomic analysis of the acetic acid stress in Issatchenkia orientalis

2020 ◽  
Vol 44 (6) ◽  
Author(s):  
Yingdi Li ◽  
Zufang Wu ◽  
Ruoyun Li ◽  
Yingjie Miao ◽  
Peifang Weng ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Proteomic Analysis ◽  
Acid Stress ◽  
Issatchenkia Orientalis

scholarly journals Sphingolipid biosynthesis upregulation by TOR complex 2–Ypk1 signaling during yeast adaptive response to acetic acid stress

2016 ◽  
Vol 473 (23) ◽  
pp. 4311-4325 ◽  
Author(s):  
Joana F. Guerreiro ◽  
Alexander Muir ◽  
Subramaniam Ramachandran ◽  
Jeremy Thorner ◽  
Isabel Sá-Correia
Keyword(s):  
Acetic Acid ◽  
Protein Kinase ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Industrial Biotechnology ◽  
Acetic Acid Tolerance ◽  
Spoilage Yeasts ◽  
Sphingolipid Biosynthesis

Acetic acid-induced inhibition of yeast growth and metabolism limits the productivity of industrial fermentation processes, especially when lignocellulosic hydrolysates are used as feedstock in industrial biotechnology. Tolerance to acetic acid of food spoilage yeasts is also a problem in the preservation of acidic foods and beverages. Thus understanding the molecular mechanisms underlying adaptation and tolerance to acetic acid stress is increasingly important in industrial biotechnology and the food industry. Prior genetic screens for Saccharomyces cerevisiae mutants with increased sensitivity to acetic acid identified loss-of-function mutations in the YPK1 gene, which encodes a protein kinase activated by the target of rapamycin (TOR) complex 2 (TORC2). We show in the present study by several independent criteria that TORC2–Ypk1 signaling is stimulated in response to acetic acid stress. Moreover, we demonstrate that TORC2-mediated Ypk1 phosphorylation and activation is necessary for acetic acid tolerance, and occurs independently of Hrk1, a protein kinase previously implicated in the cellular response to acetic acid. In addition, we show that TORC2–Ypk1-mediated activation of l-serine:palmitoyl-CoA acyltransferase, the enzyme complex that catalyzes the first committed step of sphingolipid biosynthesis, is required for acetic acid tolerance. Furthermore, analysis of the sphingolipid pathway using inhibitors and mutants indicates that it is production of certain complex sphingolipids that contributes to conferring acetic acid tolerance. Consistent with that conclusion, promoting sphingolipid synthesis by adding exogenous long-chain base precursor phytosphingosine to the growth medium enhanced acetic acid tolerance. Thus appropriate modulation of the TORC2–Ypk1–sphingolipid axis in industrial yeast strains may have utility in improving fermentations of acetic acid-containing feedstocks.

Different response to acetic acid stress inSaccharomyces cerevisiaewild-type andl-ascorbic acid-producing strains

Yeast ◽  
2013 ◽  
Vol 30 (9) ◽  
pp. 365-378 ◽  
Author(s):  
Francesca Martani ◽  
Tiziana Fossati ◽  
Riccardo Posteri ◽  
Lorenzo Signori ◽  
Danilo Porro ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Acid Stress ◽  
Different Response

scholarly journals Endogenous lycopene improves ethanol production under acetic acid stress in Saccharomyces cerevisiae

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Shuo Pan ◽  
Bin Jia ◽  
Hong Liu ◽  
Zhen Wang ◽  
Meng-Zhe Chai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Ethanol Production ◽  
Acid Stress

scholarly journals Transcriptome profile of rat genes in bone marrow-derived macrophages at different activation statuses by RNA-sequencing

Genomics ◽  
2019 ◽  
Vol 111 (4) ◽  
pp. 986-996
Author(s):  
Xue-Yan Guo ◽  
Sai-Nan Wang ◽  
Yan Wu ◽  
Yu-Hong Lin ◽  
Jie Tang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Rna Sequencing ◽  
Transcriptome Profile

Transcriptome response of Acetobacter pasteurianus Ab3 to high acetic acid stress during vinegar production

2020 ◽  
Vol 104 (24) ◽  
pp. 10585-10599
Author(s):  
Kai Xia ◽  
Chengcheng Han ◽  
Jun Xu ◽  
Xinle Liang
Keyword(s):  
Acetic Acid ◽  
Acid Stress ◽  
Acetobacter Pasteurianus ◽  
Transcriptome Response

scholarly journals Acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-induced programmed cell death

Gene ◽  
2005 ◽  
Vol 354 ◽  
pp. 93-98 ◽  
Author(s):  
Sergio Giannattasio ◽  
Nicoletta Guaragnella ◽  
Manuela Corte-Real ◽  
Salvatore Passarella ◽  
Ersilia Marra
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Acetic Acid ◽  
Programmed Cell Death ◽  
Acid Stress ◽  
Stress Adaptation

scholarly journals Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress

2011 ◽  
Vol 39 (16) ◽  
pp. 6896-6907 ◽  
Author(s):  
Nuno P. Mira ◽  
Sílvia F. Henriques ◽  
Greg Keller ◽  
Miguel C. Teixeira ◽  
Rute G. Matos ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Acetic Acid ◽  
Dna Binding ◽  
Binding Site ◽  
Acid Stress ◽  
Dna Binding Site

scholarly journals Improved Acetic Acid Resistance in Saccharomyces cerevisiae by Overexpression of theWHI2Gene Identified through Inverse Metabolic Engineering

2016 ◽  
Vol 82 (7) ◽  
pp. 2156-2166 ◽  
Author(s):  
Yingying Chen ◽  
Lisa Stabryla ◽  
Na Wei
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Metabolic Engineering ◽  
Acid Stress ◽  
Acid Resistance ◽  
Biofuel Production ◽  
Gene Target ◽  
Content Type ◽  
Inverse Metabolic Engineering ◽  
Acetic Acid Resistance

ABSTRACTDevelopment of acetic acid-resistantSaccharomyces cerevisiaeis important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge due to limited information on effective genetic perturbation targets for improving acetic acid resistance in the yeast. This study employed a genomic-library-based inverse metabolic engineering approach to successfully identify a novel gene target,WHI2(encoding a cytoplasmatic globular scaffold protein), which elicited improved acetic acid resistance inS. cerevisiae. Overexpression ofWHI2significantly improved glucose and/or xylose fermentation under acetic acid stress in engineered yeast. TheWHI2-overexpressing strain had 5-times-higher specific ethanol productivity than the control in glucose fermentation with acetic acid. Analysis of the expression ofWHI2gene products (including protein and transcript) determined that acetic acid induced endogenous expression of Whi2 inS. cerevisiae. Meanwhile, thewhi2Δ mutant strain had substantially higher susceptibility to acetic acid than the wild type, suggesting the important role of Whi2 in the acetic acid response inS. cerevisiae. Additionally, overexpression ofWHI2and of a cognate phosphatase gene,PSR1, had a synergistic effect in improving acetic acid resistance, suggesting that Whi2 might function in combination with Psr1 to elicit the acetic acid resistance mechanism. These results improve our understanding of the yeast response to acetic acid stress and provide a new strategy to breed acetic acid-resistant yeast strains for renewable biofuel production.

scholarly journals Transcriptomic analysis of bacteriocin synthesis and stress response in Lactobacillus paracasei HD1.7 under acetic acid stress

LWT ◽  
2021 ◽  
pp. 112897
Author(s):  
Jie Kang ◽  
Xiaohang Zhou ◽  
Wen Zhang ◽  
Fangyi Pei ◽  
Jingping Ge
Keyword(s):  
Acetic Acid ◽  
Stress Response ◽  
Acid Stress ◽  
Transcriptomic Analysis ◽  
Lactobacillus Paracasei
Sign in / Sign up

Export Citation Format

Share Document