The Importance of Sample Preparation and Storage in Glutathione Analysis

1993 ◽  
Vol 211 (2) ◽  
pp. 183-187 ◽  
Author(s):  
J.C. Roberts ◽  
D.J. Francetic
Keyword(s):  
Sample Preparation ◽  
And Storage

Human Urine as Test Material in1H NMR-Based Metabonomics:  Recommendations for Sample Preparation and Storage

2007 ◽  
Vol 79 (3) ◽  
pp. 1181-1186 ◽  
Author(s):  
Michael Lauridsen ◽  
Steen H. Hansen ◽  
Jerzy W. Jaroszewski ◽  
Claus Cornett
Keyword(s):  
Sample Preparation ◽  
Human Urine ◽  
Test Material ◽  
And Storage

Empfehlungen zur qualitätsgesicherten Durchführung von Nukleinsäure-Amplifikations - Testungen (NAT) von Blutprodukten. Quality assurance in nucleic acid amplification testing (NAT) of blood products

2005 ◽  
Vol 29 (2) ◽  
Author(s):  
Willi K. Roth
Keyword(s):  
Quality Control ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Nucleic Acids ◽  
Internal Control ◽  
Control Measures ◽  
Nucleic Acid Amplification ◽  
Blood Products ◽  
Whole Process ◽  
And Storage

AbstractEuropean manufacturers of plasma products and German blood transfusion services were the first to introduce nucleic acid amplification testing (NAT) of blood products in the mid-1990s. Their primary goal was to increase the safety of blood by closing as far as possible the diagnostic window, which exists after the onset of viral infection until the appearance of the first detectable antibodies. Sample preparation, transport and storage are crucial steps in a quality-controlled PCR. Sensitivity and contamination rates highly depend on the sample preparation and storage techniques. Anticoagulants must be selected carefully because some may inhibit the PCR. Dilution of samples by pooling needs to be considered and should be compensated for by subsequent virus enrichment procedures, e.g. centrifugation. The whole process of sample preparation, pooling and virus enrichment must be validated and quality control measures must be implemented. Reagents for the extraction of viral nucleic acids should not pose any risk to the laboratory staff. Nevertheless, the reagents should be highly efficient in liberating viral nucleic acids at high yield and purity for the following amplification reactions. At this critical stage, quality control measures should guarantee an efficient extraction process and contain potential sources of contaminations. Several methods are available for the amplification of nucleic acids. PCR is the most common, especially in in-house assays. The amplification of nucleic acids should be performed as far as possible in a closed system, which may be guaranteed best by real-time PCR approaches. Reaction tubes need never be opened during the amplification because detection can be performed through the closed tube. Amplicons that could contaminate the following PCR reactions will not be released. It is of great importance to blood transfusion services to guarantee that negative results un-equivocally indicate virus negative blood donations. Therefore, internal control sequences should be implemented in each individual PCR reaction in order to monitor that the individual PCR has worked correctly. Besides internal control sequences, external negative and positive controls should be implemented in each PCR run to demonstrate false positive reactions as well as to monitor pre-PCR processes like virus enrichment and extraction. The whole process needs to be validated according to the criteria set in national guidelines or by national authorities. External quality assessment programs are highly recommended.

scholarly journals An open-source cryo-storage solution

2017 ◽  
Author(s):  
Eveline Ultee ◽  
Fred Schenkel ◽  
Wen Yang ◽  
Susanne Brenzinger ◽  
Jamie S. Depelteau ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Sample Preparation ◽  
Structural Biology ◽  
Storage Capacity ◽  
Storage System ◽  
Cost Effective ◽  
Wait Times ◽  
Sample Storage ◽  
Cryo Electron Microscopy ◽  
And Storage

AbstractThe field of cryo-electron microscopy is a rapidly growing method in structural biology. With this development, access to cryo-EM facilities becomes a bottleneck that results in long wait times between sample preparation and data acquisition. To improve sample storage, we developed a cryo-storage system with a more efficient and larger storage capacity that enables cryo-sample storage in a highly organized manner. This system is simple to use, cost-effective and easily adaptable for any type of grid box and storage dewar and any size cryo-EM laboratory.

The effect of sample preparation and storage on maternal triple-marker screening

1995 ◽  
Vol 85 (6) ◽  
pp. 919-923 ◽  
Author(s):  
M LANTZ ◽  
W OBRIEN ◽  
M WILLIAMS
Keyword(s):  
Sample Preparation ◽  
And Storage

scholarly journals Erratum to “Whole bovine blood use in forensic research: Sample preparation and storage considerations” [Sci. Justice 61 (3) (2021) 214–220]

2021 ◽  
Vol 61 (6) ◽  
pp. 667-668
Author(s):  
Amanda Orr ◽  
Rebecca Gualdieri ◽  
Marie-Laurence Cossette ◽  
Aaron B.A. Shafer ◽  
Theresa Stotesbury
Keyword(s):  
Sample Preparation ◽  
Bovine Blood ◽  
Forensic Research ◽  
And Storage

scholarly journals Validation of Methods to Assess the Immunoglobulin Gene Repertoire in Tissues Obtained from Mice on the International Space Station

2020 ◽  
Vol 5 (1) ◽  
pp. 2-23 ◽  
Author(s):  
Trisha A. Rettig ◽  
Claire Ward ◽  
Michael J. Pecaut ◽  
Stephen K. Chapes
Keyword(s):  
Sample Preparation ◽  
International Space Station ◽  
Gene Segment ◽  
Space Station ◽  
Gene Repertoire ◽  
Immunoglobulin Gene ◽  
Data Set ◽  
International Space ◽  
The Impact ◽  
And Storage

AbstractSpaceflight is known to affect immune cell populations. In particular, splenic B-cell numbers decrease during spaceflight and in ground-based physiological models. Although antibody isotype changes have been assessed during and after spaceflight, an extensive characterization of the impact of spaceflight on antibody composition has not been conducted in mice. Next Generation Sequencing and bioinformatic tools are now available to assess antibody repertoires. We can now identify immunoglobulin gene-segment usage, junctional regions, and modifications that contribute to specificity and diversity. Due to limitations on the International Space Station, alternate sample collection and storage methods must be employed. Our group compared Illumina MiSeq® sequencing data from multiple sample preparation methods in normal C57Bl/6J mice to validate that sample preparation and storage would not bias the outcome of antibody repertoire characterization. In this report, we also compared sequencing techniques and a bioinformatic workflow on the data output when we assessed the IgH and Igκ variable gene usage. Our bioinformatic workflow has been optimized for Illumina HiSeq® and MiSeq® datasets, and is designed specifically to reduce bias, capture the most information from Ig sequences, and produce a data set that provides other data mining options.

scholarly journals The Forgotten Variable: Effects of Sample Preparation and Storage on Geochemistry of Invertebrate Skeletal Carbonate

2018 ◽  
Vol 2 ◽  
pp. e26288
Author(s):  
Abigail Smith ◽  
Marcus Key, Jr ◽  
Jennifer Loxton ◽  
Anna Righi ◽  
Jeffrey Forrester ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Considerable Effect ◽  
Detectable Effect ◽  
Low Concentrations ◽  
Isotope Concentration ◽  
Sample Composition ◽  
Sample Dissolution ◽  
High Concentrations ◽  
Carbonate Composition ◽  
And Storage

Standard sample preparation often includes cleaning, bleaching, preservation and storage techniques that may affect temperate invertebrate skeletal carbonate composition and integrity. Here we report on a series of interlinked studies and their key results. Rinsing with tap or deionised water has little detectable effect on sample composition and integrity. Bleaching with sodium hypochlorite, especially at low concentrations and for short times, results in no change in mineralogy or stable isotopes. High concentrations of bleach, and oxidation using H2O2, result in sample dissolution and the leaching of Mg from high-Mg samples. H2O2 has a significant effect on ∂18O stable isotope concentration. Chemical removal of organic material is often not necessary. Ultrasonication of samples results in loss of Mg and skeletal material generally, especially from high-Mg specimens. The combination of ultrasound with bleaching (a common shortcut) is even worse. Roasting of specimens to remove organic carbon causes dramatic changes in composition and cannot be recommended. New studies on the effect of storage in various preservatives and conditions are underway. In order to examine longer-term effects of storage, we have been mining museum collections. This collecting activity is ongoing and we would be glad to hear about material we have missed. Sample preparation and storage can have considerable effect on subsequent chemical investigations, and must form part of research planning, collection management, and sample labelling.

Sign in / Sign up

Export Citation Format

Share Document