Influence of sample preparation and storage on theophylline concentrations in biological fluids

1982 ◽  
Vol 10 (2) ◽  
pp. 177-180 ◽  
Author(s):  
J Jonkman
Keyword(s):  
Sample Preparation ◽  
Biological Fluids ◽  
And Storage

scholarly journals Stability of Plasma Protein Composition in Dried Blood Spot during Storage

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1500
Author(s):  
Kristina A. Malsagova ◽  
Alexander A. Stepanov ◽  
Arthur T. Kopylov ◽  
Dmitry V. Enikeev ◽  
Natalia V. Potoldykova ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Biological Fluids ◽  
Protein Composition ◽  
Clinical Analysis ◽  
Dried Blood Spot ◽  
Different Temperatures ◽  
Preliminary Preparation ◽  
Molecular Components ◽  
And Storage ◽  
Blood Spot

Dried blood spot (DBS) technology has become a promising utility for the transportation and storage of biological fluids aimed for the subsequent clinical analysis. The basis of the DBS method is the adsorption of the components of a biological sample onto the surface of a membrane carrier, followed by drying. After drying, the molecular components of the biosample (nucleic acids, proteins, and metabolites) can be analyzed using modern omics, immunological, or genomic methods. In this work, we investigated the safety of proteins on a membrane carrier by tryptic components over time and at different temperatures (+4, 0, 25 °C) and storage (0, 7, 14, and 35 days). It was shown that the choice of a protocol for preliminary sample preparation for subsequent analytical molecular measurements affects the quality of the experimental results. The protocol for preliminary preparation of a biosample directly in a membrane carrier is preferable compared to the protocol with an additional stage of elution of molecular components before the sample preparation procedures. It was revealed that the composition of biosamples remains stable at a temperature of −20 and +4 °C for 35 days of storage, and at +25 °C for 14 days.

scholarly journals The Use of Infrared Spectrophotometry for Measuring Body Water Spaces

1999 ◽  
Vol 45 (7) ◽  
pp. 1077-1081 ◽  
Author(s):  
Graham Jennings ◽  
Leslie Bluck ◽  
Antony Wright ◽  
Marinos Elia
Keyword(s):  
Sample Preparation ◽  
Total Body Water ◽  
Biological Fluids ◽  
Deuterium Oxide ◽  
Body Water ◽  
The Body ◽  
Infrared Spectrophotometry ◽  
Significant Difference ◽  
Total Body ◽  
Novel Algorithm

Abstract Background: The conventional method of measuring total body water by the deuterium isotope dilution method uses gas isotope ratio mass spectrometry (IRMS), which is both expensive and time-consuming. We investigated an alternative method, using Fourier transform infrared spectrophotometry (FTIR), which uses less expensive instrumentation and requires little sample preparation. Method: Total body water measurements in human subjects were made by obtaining plasma, saliva, and urine samples before and after oral dosing with 1.5 mol of deuterium oxide. The enrichments of the body fluids were determined from the FTIR spectra in the range 1800–2800 cm−1, using a novel algorithm for estimation of instrumental response, and by IRMS for comparison. Results: The CV (n = 5) for repeat determinations of deuterium oxide in biological fluids and calibrator solutions (400–1000 μmol/mol) was found to be in the range 0.1–0.9%. The use of the novel algorithm instead of the integration routines supplied with the instrument gave at least a threefold increase in precision, and there was no significant difference between the results obtained with FTIR and those obtained with IRMS. Conclusion: This improved infrared method for measuring deuterium enrichment in plasma and saliva requires no sample preparation, is rapid, and has potential value to the clinician.

Human Urine as Test Material in1H NMR-Based Metabonomics:  Recommendations for Sample Preparation and Storage

2007 ◽  
Vol 79 (3) ◽  
pp. 1181-1186 ◽  
Author(s):  
Michael Lauridsen ◽  
Steen H. Hansen ◽  
Jerzy W. Jaroszewski ◽  
Claus Cornett
Keyword(s):  
Sample Preparation ◽  
Human Urine ◽  
Test Material ◽  
And Storage

scholarly journals Chemical and Toxicological Analysis of Antiretroviral Drugs

2019 ◽  
Vol 8 (4) ◽  
pp. 53-60
Author(s):  
T. N. Komarov ◽  
M. V. Belova ◽  
D. D. Stolyarova ◽  
I. E. Shohin ◽  
D. S. Bogdanova ◽  
...  
Keyword(s):  
Side Effects ◽  
Sample Preparation ◽  
Highly Active Antiretroviral Therapy ◽  
High Performance ◽  
Biological Fluids ◽  
Screening Method ◽  
Acute Poisoning ◽  
Antiretroviral Drugs ◽  
Precipitation Method ◽  
Relevant Today

Introduction. Human Immunodeficiency Virus (HIV) is one of the main socially significant infection all over the world. HIV-positive patients take medical care, including antiretroviral drugs (ARVs) pharmacotherapy. Like all drugs, ARVs have lots of side effects that should be taken when prescribing drugs as part of highly active antiretroviral therapy. There are many cases when side effects of ARVs caused patients to enter the toxicology department. Therefore, the development of new methods for the analysis of ARV in biological fluids for the timely diagnosis of treatment of poisoning of this group of drugs is relevant today.Aim. The aim of this study is development of screening analysis of atazanavir, abacavir, nevirapine, ritonavir, lopinavir, zidovudine, darunavir and efavirenz in the urine to identify these drugs as possible toxicants for poisoning by high-performance liquid chromatography with tandem massselective detection (HPLC-MS/MS).Materials and methods. Identification of ARV was performed by HPLC-MS/MS. Methanol precipitation method was used as a sample preparation.Results and discussion. The optimal conditions for sample preparation, chromatographic separation, and mass-spectrometric detection were selected to determine the studied ARVs. This method was tested on urine samples from patients in the Department of Acute Poisoning and Somatopsychiatric Disorders (OOSPD) with acute ARV poisoning.Conclusion. This screening method for analyse atazanavir, abacavir, nevirapine, ritonavir, lopinavir, zidovudine, darunavir and efavirenz in human urine has been developed by HPLC-MS/MS. The developed method can be used to identify these drugs as possible toxicants in case of poisoning. The prospect for the development of the topic is the inclusion of new molecules in the method and quantitative determination of the studied ARVs. 

Emission Spectrometric Determination of Trace Elements in Biological Fluids

1971 ◽  
Vol 25 (1) ◽  
pp. 53-56 ◽  
Author(s):  
William Niedermeier ◽  
James H. Griggs ◽  
Richard S. Johnson
Keyword(s):  
Experimental Data ◽  
Trace Elements ◽  
Sample Preparation ◽  
Blood Serum ◽  
Biological Fluids ◽  
Spectrometric Method ◽  
Direct Reading ◽  
Iron Aluminum ◽  
Dc Arc

An emission spectrometric method of analysis is described, in which trace quantities of copper, iron, aluminum, barium, manganese, nickel, cesium, tin, strontium, chromium, zinc, lead, molybdenum, and cadmium were determined in blood serum. The sample preparation, starting with 2.0 ml of blood serum, is discussed in detail. The source of excitation was a 10 A dc arc. Quantitation was achieved with a direct reading emission spectrometer. The metal concentration, in micrograms per 100 ml of blood serum, was calculated from the experimental data by means of a computer.

scholarly journals Challenges for Biomarker Discovery in Body Fluids Using SELDI-TOF-MS

2010 ◽  
Vol 2010 ◽  
pp. 1-15 ◽  
Author(s):  
Muriel De Bock ◽  
Dominique de Seny ◽  
Marie-Alice Meuwis ◽  
Jean-Paul Chapelle ◽  
Edouard Louis ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Biological Fluids ◽  
Protein Profiling ◽  
Sample Collection ◽  
Sample Handling ◽  
Biomarker Identification ◽  
The Past ◽  
Set Up ◽  
And Storage ◽  
Tof Ms

Protein profiling using SELDI-TOF-MS has gained over the past few years an increasing interest in the field of biomarker discovery. The technology presents great potential if some parameters, such as sample handling, SELDI settings, and data analysis, are strictly controlled. Practical considerations to set up a robust and sensitive strategy for biomarker discovery are presented. This paper also reviews biological fluids generally available including a description of their peculiar properties and the preanalytical challenges inherent to sample collection and storage. Finally, some new insights for biomarker identification and validation challenges are provided.

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