Quantitative Determination of Gene Expression by Competitive Reverse Transcription–Polymerase Chain Reaction in Degraded RNA Samples

1997 ◽  
Vol 251 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Dan Tong ◽  
Christian Schneeberger ◽  
Sepp Leodolter ◽  
Robert Zeillinger
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Quantitative Determination ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Study of the Alteration of Gene Expression in Adipose Tissue of Diet-Induced Obese Mice by Microarray and Reverse Transcription-Polymerase Chain Reaction Analyses

Endocrinology ◽  
2003 ◽  
Vol 144 (11) ◽  
pp. 4773-4782 ◽  
Author(s):  
R. C. Moraes ◽  
A. Blondet ◽  
K. Birkenkamp-Demtroeder ◽  
J. Tirard ◽  
T. F. Orntoft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Adipose Tissue ◽  
Reverse Transcription ◽  
Obese Mice ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals The contribution of increased collagen synthesis to human glomerulosclerosis: a quantitative analysis of alpha 2IV collagen mRNA expression by competitive polymerase chain reaction.

1992 ◽  
Vol 176 (6) ◽  
pp. 1571-1576 ◽  
Author(s):  
E P Peten ◽  
L J Striker ◽  
M A Carome ◽  
S J Elliott ◽  
C W Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Collagen Synthesis ◽  
Type Iv Collagen ◽  
Chain Reaction ◽  
Collagen Mrna ◽  
Type Iv ◽  
Polymerase Chain

We previously reported that one of the main components of the sclerotic material in human glomerular diseases was type IV collagen. In this study we examined the contribution of increased synthesis to this process at the gene expression level. Sufficient material has not been available to study type IV collagen synthesis by normal or sclerotic glomeruli in humans. We took advantage of the availability of nephrectomy specimens from patients with renal carcinoma, and of the observation that approximately 50% of these patients develop varying degrees of glomerulosclerosis. We microdissected glomeruli from 10 patients and analyzed them using in situ reverse transcription coupled with polymerase chain reaction (PCR) analyses (in situ RT-PCR). alpha 2IV collagen mRNA, after reverse transcription into cDNA, was detected in all patients and appeared to be increased in those with glomerulosclerosis (n = 5). A competitive PCR assay was developed to quantitate this change. There was an average 3.7-fold increase in glomerular type IV collagen cDNA in patients with significant sclerosis. This change was not due to an increased number of glomerular cells. Thus, glomerulosclerosis in humans is associated with an elevation of glomerular type IV collagen gene expression, suggesting that increased synthesis of type IV collagen may represent one component of this process.

Detection of canine cytokine gene expression by reverse transcription-polymerase chain reaction

1999 ◽  
Vol 69 (2-4) ◽  
pp. 121-126 ◽  
Author(s):  
E. Pinelli ◽  
S.Y. van der Kaaij ◽  
R. Slappendel ◽  
C. Fragio ◽  
E.J. Ruitenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Chain Reaction ◽  
Polymerase Chain
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