Cloning and Characterization of a pH-Sensing Regulatory Factor That Modulates Transport Activity of the Human H+/Peptide Cotransporter, PEPT1

1997 ◽  
Vol 237 (3) ◽  
pp. 577-582 ◽  
Author(s):  
Hideyuki Saito ◽  
Hideyuki Motohashi ◽  
Mayumi Mukai ◽  
Ken-ichi Inui
Keyword(s):  
Ph Sensing ◽  
Transport Activity ◽  
Regulatory Factor

Characterization of Na(+)-H+ antiporter activity associated with human cheek epithelial cells

1994 ◽  
Vol 267 (1) ◽  
pp. C84-C93 ◽  
Author(s):  
E. J. McMurchie ◽  
S. L. Burnard ◽  
G. S. Patten ◽  
E. J. Lee ◽  
R. A. King ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Subjects ◽  
Maximum Velocity ◽  
Hill Coefficient ◽  
Transport Activity ◽  
Uptake Activity ◽  
Dimethyl Amiloride ◽  
Mouth Wash ◽  
Na Uptake

Na+ transport activity was characterized in human cheek epithelial cells obtained from normotensive adult subjects. The cells were isolated using a mouth-wash procedure and assayed for Na+ uptake using a radioactive (22Na+) rapid filtration assay. Cheek cells displayed proton-dependent Na+ uptake activity that was dependent on the magnitude of the externally directed proton gradient measured using the fluorescent probe 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein to determine intracellular pH. Amiloride, ethylisopropylamiloride (EIPA), 5-(N,N-dimethyl)-amiloride, 5-(N-methyl-N-isobutyl)-amiloride (MIA), and 5-(N,N-hexamethylene)-amiloride (NNHA) all inhibited proton-dependent Na+ uptake, with MIA, EIPA, and NNHA being the most potent. The Michaelis constant (Km) for extracellular Na+ was 5.7 mM, while the maximum velocity for Na(+)-H+ antiporter activity was 4.3 nmol Na+.mg protein-1.30s-1. The Km for intracellular H+ was 0.17 microM, with a Hill coefficient of 0.7. Stimulation by ouabain and inhibition by bumetanide of cheek cell proton-dependent Na+ uptake indicated only relatively low activities of Na(+)-K(+)-ATPase and Na(+)-K(+)-2Cl- cotransport, respectively. These results are consistent with the presence of Na(+)-H+ antiporter activity in cheek cells. Cheek cells therefore provide a convenient, relatively noninvasive source of tissue for examining Na(+)-H+ antiporter activity in human subjects.

Synthesis and characterization of hybrid matrix with encapsulated organic sensing dyes for pH sensing application

2014 ◽  
Vol 20 (6) ◽  
pp. 4408-4414 ◽  
Author(s):  
Shumaila Islam ◽  
Rosly Rahman ◽  
Zulkafli Othaman ◽  
Saira Riaz ◽  
Shahzad Naseem
Keyword(s):  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Hybrid Matrix ◽  
Sensing Application

Characterization of the interferon regulatory factor 3-mediated antiviral response in a cell line deficient for IFN production

2009 ◽  
Vol 46 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Tracy Chew ◽  
Ryan Noyce ◽  
Susan E. Collins ◽  
Meaghan H. Hancock ◽  
Karen L. Mossman
Keyword(s):  
Cell Line ◽  
Interferon Regulatory Factor ◽  
Antiviral Response ◽  
Regulatory Factor ◽  
Interferon Regulatory Factor 3 ◽  
A Cell
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