Human Fetal Skin Fibroblast Migration Stimulated by the Autocrine Growth Factor bFGF Is Mediated by Phospholipase A2 via Arachidonic Acid without the Involvement of Pertussis Toxin-Sensitive G-Protein

2000 ◽  
Vol 272 (3) ◽  
pp. 648-652 ◽  
Author(s):  
Hiroshi Kondo ◽  
Yumiko Yonezawa
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Phospholipase A2 ◽  
G Protein ◽  
Pertussis Toxin ◽  
Skin Fibroblast ◽  
Fetal Skin ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Fibroblast Migration

A role for G-proteins in the epidermal growth factor stimulation of phospholipase A2 in rat kidney mesangial cells

1990 ◽  
Vol 10 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Nashrudeen Hack ◽  
Paula Clayman ◽  
Karl Skorecki
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Phospholipase A2 ◽  
G Protein ◽  
Mesangial Cells ◽  
Rat Kidney ◽  
Glomerular Mesangial Cells ◽  
Epidermal Growth ◽  
Stimulation Of

We have previously demonstrated phospholipase C (PLC) independent activation of phospholipase A2(PLA2) by epidermal growth factor (EGF) in glomerular mesangial cells in culture. In the current study using glass beads to permeabilize [3H]- or [14C]-arachidonate labelled mesangial cells we demonstrate that guanine nucleotides modulate the EGF-mediated stimulation of arachidonic acid release (75% inhibition with 100 μM GDPβS and 108% augmentation with 100 μM GTPγS). GTPγS alone stimulated both the release of free arachidonic acid and production of diacylglycerol (DAG), while EGF itself neither stimulated DAG nor augmented the DAG response to GTPγS. These findings suggest the intermediacy of a G-protein in PLC-independent stimulation of PLA2 by a growth factor, and provide a model system for determining the relationship between G-protein intermediacy and the intrinsic tyrosine kinase activity of the growth factor receptor.

Group II phospholipase A2 as an autocrine growth factor mediating interleukin-1 action on mesangial cells

1997 ◽  
Vol 1345 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Akira Wada ◽  
Hiromasa Tojo ◽  
Toshihiro Sugiura ◽  
Yoshihiro Fujiwara ◽  
Takenobu Kamada ◽  
...  
Keyword(s):  
Growth Factor ◽  
Phospholipase A2 ◽  
Mesangial Cells ◽  
Interleukin 1 ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Group Ii

Embryo-derived platelet activating factor: interactions with the arachidonic acid cascade and the establishment and maintenance of pregnancy

1990 ◽  
Vol 2 (5) ◽  
pp. 423 ◽  
Author(s):  
C O'Neill ◽  
X Wells ◽  
K Battye
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Platelet Activating Factor ◽  
Primary Source ◽  
Early Embryo ◽  
Autocrine Growth ◽  
Proinflammatory Response ◽  
Autocrine Growth Factor

Two classes of potent lipid mediators are produced by the mouse and human pre-embryo: platelet activating factor (PAF) and prostaglandins (PGs). This paper reviews the evidence for their production by the pre-embryo and for their role in embryo development and the successful establishment of pregnancy. The biosynthesis of PAF and arachidonic acid may be linked, the synthesis of PAF resulting in the generation of arachidonic acid with its subsequent conversion to prostaglandins. Pharmacological inhibitor studies show that a major site of action of PAF is the embryo itself, acting as an embryonic autocrine growth factor, whereas PGs appear to act primarily on maternal tissues although they do modulate some aspects of early embryo metabolism. It is the maternal tissues that are the primary source of PG production in early pregnancy. Maternal PGs have a variety of functions including involvement in the proinflammatory response of early pregnancy and the control of corpus luteum function. In the ewe, pregnancy is associated with an attenuation of oxytocin-induced production of the luteolysin, prostaglandin F2 alpha (PGF2 alpha). PAF can mimic the effect of pregnancy, preventing the release of PGF2 alpha in response to exogenous oxytocin and, when administered into the uterine lumen, extending the life span of the corpus luteum. Thus, embryo-derived PAF appears to have an essential role in the establishment of pregnancy by acting as an autocrine growth factor for the embryo and by exerting a variety of effects on maternal physiology, including modulating maternal prostaglandin secretion and action.

scholarly journals The receptors for ATP and fMetLeuPhe are independently coupled to phospholipases C and A2 via G-protein(s). Relationship between phospholipase C and A2 activation and exocytosis in HL60 cells and human neutrophils

1989 ◽  
Vol 263 (3) ◽  
pp. 715-723 ◽  
Author(s):  
S Cockcroft ◽  
J Stutchfield
Keyword(s):  
Arachidonic Acid ◽  
Phospholipase A2 ◽  
Phospholipase C ◽  
G Protein ◽  
Pertussis Toxin ◽  
Human Neutrophils ◽  
Ionophore A23187 ◽  
Arachidonic Acid Release ◽  
Hl60 Cells ◽  
Acid Release

The relationship between phospholipase A2 and C activation and secretion was investigated in intact human neutrophils and differentiated HL60 cells. Activation by either ATP or fMetLeuPhe leads to [3H]arachidonic acid release into the external medium from prelabelled cells. This response was inhibited when the cells were pretreated with pertussis toxin. When the [3H]arachidonic acid-labelled cells were stimulated with fMetLeuPhe, ATP or Ca2+ ionophore A23187, and the lipids analysed by t.l.c., the increase in free fatty acid was accompanied by decreases in label from phosphatidylinositol and phosphatidylcholine. Moreover, incorporation of label into triacylglycerol and to a lesser extent phosphatidylethanolamine was evident. Activation of secretion was evident with ATP and fMetLeuPhe but not with A23187. The pharmacological specificity of the ATP receptor in HL60 cells was investigated by measuring secretion of beta-glucuronidase, formation of inositol phosphatases and release of [3H]arachidonic acid. External addition of ATP, UTP, ITP, adenosine 5′-[gamma-thio]triphosphate (ATP[S]), adenosine 5′-[beta gamma-imido]triphosphate (App[NH]p), XTP, CTP, GTP, 8-bromo-ATP and guanosine 5′-[gamma-thio]triphosphate (GTP[S]) to intact HL60 cells stimulated inositol phosphate production, but only the first five nucleotides were effective at stimulating secretion or [3H]arachidonic acid release. In human neutrophils, addition of ATP, ITP, UTP and ATP[S] also stimulated secretion from specific and azurophilic granules, and this was accompanied by increases in cytosolic Ca2+ and in [3H]arachidonic acid release. The addition of phorbol 12-myristate 13-acetate (PMA; 1 nM) prior to the addition of either fMetLeuPhe or ATP led to inhibition of phospholipase C activity. In contrast, this had no effect on phospholipase A2 activation, whilst secretion was potentiated. Phospholipase A2 activation by either agonist was dependent on an intact cell metabolism, as was secretion. It is concluded that (1) activation of phospholipase C does not always lead to activation of phospholipase A2, (2) phospholipase A2 is coupled to the receptor independently of phospholipase C via a pertussis-toxin-sensitive G-protein and (3) for secretion to take place, the receptor has to activate both phospholipases C and A2.

scholarly journals Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes.

1994 ◽  
Vol 269 (31) ◽  
pp. 20060-20066 ◽  
Author(s):  
K. Hashimoto ◽  
S. Higashiyama ◽  
H. Asada ◽  
E. Hashimura ◽  
T. Kobayashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Keratinocytes ◽  
Heparin Binding ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Epidermal Growth

Cyclic Mechanical Deformation Stimulates Human Lung Fibroblast Proliferation and Autocrine Growth Factor Activity

1993 ◽  
Vol 9 (2) ◽  
pp. 126-133 ◽  
Author(s):  
Jill E. Bishop ◽  
John J. Mitchell ◽  
P. Marlene Absher ◽  
Linda Baldor ◽  
Henry A. Geller ◽  
...  
Keyword(s):  
Growth Factor ◽  
Human Lung ◽  
Mechanical Deformation ◽  
Lung Fibroblast ◽  
Fibroblast Proliferation ◽  
Factor Activity ◽  
Autocrine Growth ◽  
Human Lung Fibroblast ◽  
Autocrine Growth Factor

Stimulation of the multiplication of Micrococcus luteus by an autocrine growth factor

1999 ◽  
Vol 172 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Galina V. Mukamolova ◽  
Svetlana S. Kormer ◽  
Douglas B. Kell ◽  
A. S. Kaprelyants
Keyword(s):  
Growth Factor ◽  
Micrococcus Luteus ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Stimulation Of
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