Real-time Quantitative PCR for Retrovirus-like Particle Quantification in CHO Cell Culture

To evaluate the persistence of infectious virus after heating, mussels contaminated with a rotavirus strain were prepared following the French recipe moules marinières (mariner's mussels). Rotavirus was then quantified by real-time quantitative PCR (RT-qPCR) and a cell culture infectivity assay. Results showed the persistence of infectious virus after 3 min of cooking. After 5 min, when no infectious virus could be detected, the RT-qPCR approach showed a 1-log decrease compared with concentrations detected after 1 min of cooking.

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Quantification of Matrix Protein mRNAs Expression During Mineralized Tissue Formation in Rat Marrow Cell Culture by a Real Time Quantitative PCR Method

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.192-195.367 ◽

2000 ◽

Vol 192-195 ◽

pp. 367-372

Author(s):

Y. Dohi ◽

Hideo Nakajima ◽

Hajime Ohgushi ◽

K. Yonemasu

Keyword(s):

Cell Culture ◽

Real Time ◽

Quantitative Pcr ◽

Matrix Protein ◽

Marrow Cell ◽

Tissue Formation ◽

Mineralized Tissue ◽

Real Time Quantitative Pcr ◽

Pcr Method

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Evaluation of the inactivation of human Coxsackievirus by thermophilic and mesophilic anaerobic digestion using integrated cell culture and reverse transcription real-time quantitative PCR

Water Research ◽

10.1016/j.watres.2013.04.046 ◽

2013 ◽

Vol 47 (13) ◽

pp. 4259-4264 ◽

Cited By ~ 2

Author(s):

Tiejun Gao ◽

Yupin Tong ◽

Ming Cao ◽

Xiaomei Li ◽

Xiaoli Pang

Keyword(s):

Cell Culture ◽

Anaerobic Digestion ◽

Real Time ◽

Quantitative Pcr ◽

Reverse Transcription ◽

Real Time Quantitative Pcr

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Selection of stable reference genes for real-time quantitative PCR in honey bee pesticide toxicity studies

Journal of Apicultural Research ◽

10.1080/00218839.2021.1950972 ◽

2021 ◽

pp. 1-11

Author(s):

Sanghyeon Kim ◽

Susie Cho ◽

Si Hyeock Lee

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Honey Bee ◽

Reference Genes ◽

Pesticide Toxicity ◽

Real Time Quantitative Pcr ◽

Toxicity Studies ◽

Selection Of

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A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas

European Food Research and Technology ◽

10.1007/s00217-008-0935-6 ◽

2008 ◽

Vol 228 (2) ◽

pp. 301-309 ◽

Cited By ~ 15

Author(s):

Wentao Xu ◽

Weibin Bai ◽

Feng Guo ◽

Yunbo Luo ◽

Yanfang Yuan ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Reference Gene ◽

Pcr Detection ◽

Specific Gene ◽

Real Time Quantitative Pcr ◽

Endogenous Reference Gene ◽

Endogenous Reference

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Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Harmful Algae ◽

10.1016/j.hal.2012.03.004 ◽

2012 ◽

Vol 17 ◽

pp. 83-91 ◽

Cited By ~ 41

Author(s):

Jian Yuan ◽

Tiezhu Mi ◽

Yu Zhen ◽

Zhigang Yu

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Rapid Detection ◽

Karenia Mikimotoi ◽

Real Time Quantitative Pcr ◽

Quantification Method ◽

Detection And Quantification

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Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungusPochonia chlamydosporia

Annals of Applied Biology ◽

10.1111/j.1744-7348.2009.00333.x ◽

2009 ◽

Vol 155 (1) ◽

pp. 143-152 ◽

Cited By ~ 20

Author(s):

S.D. Atkins ◽

B. Peteira ◽

I.M. Clark ◽

B.R. Kerry ◽

P.R. Hirsch

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Real Time Quantitative Pcr

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Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

Applied and Environmental Microbiology ◽

10.1128/aem.00965-06 ◽

2006 ◽

Vol 72 (12) ◽

pp. 7894-7896 ◽

Cited By ~ 193

Author(s):

Silvia Bofill-Mas ◽

Nestor Albinana-Gimenez ◽

Pilar Clemente-Casares ◽

Ayalkibet Hundesa ◽

Jesus Rodriguez-Manzano ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

High Stability ◽

Wastewater Sludge ◽

Human Polyomavirus ◽

Sewage Effluent ◽

Viral Contamination ◽

Human Adenoviruses ◽

Urban Sewage ◽

Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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