scholarly journals Characterization of Wild-Type Human Medium-Chain Acyl-CoA Dehydrogenase (MCAD) and Mutant Enzymes Present in MCAD-Deficient Patients by Two-Dimensional Gel Electrophoresis: Evidence for Posttranslational Modification of the Enzyme

1994 ◽  
Vol 52 (1) ◽  
pp. 36-44 ◽  
Author(s):  
P. Bross ◽  
T.G. Jensen ◽  
B.S. Andresen ◽  
M. Kjeldsen ◽  
A. Nandy ◽  
...  
Keyword(s):  
Posttranslational Modification ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Wild Type ◽  
Two Dimensional Gel Electrophoresis ◽  
Medium Chain ◽  
Chain Acyl

Characterization of antithrombin III from human plasma by two-dimensional gel electrophoresis and capillary electrophoretic methods

2003 ◽  
Vol 24 (24) ◽  
pp. 4282-4290 ◽  
Author(s):  
Leopold Kremser ◽  
Andrea Brückner ◽  
Andrea Heger ◽  
Tom Grunert ◽  
Andrea Buchacher ◽  
...  
Keyword(s):  
Human Plasma ◽  
Gel Electrophoresis ◽  
Antithrombin Iii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Capillary Electrophoretic

Characterization of Enterobacter cloacae under phoxim stress by two-dimensional gel electrophoresis

2015 ◽  
Vol 20 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Dijun Zhang ◽  
Jiajie Xu ◽  
Weina He ◽  
Qianqian Tong ◽  
Liping Chen ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Partial characterization of chromosomal proteins tightly bound to chicken erythroid DNA

1985 ◽  
Vol 63 (8) ◽  
pp. 824-829
Author(s):  
C. C. Liew ◽  
Peter C. Hentzen ◽  
Isaac Bekhor
Keyword(s):  
Amino Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Basic Amino Acid ◽  
Two Dimensional ◽  
Amino Acid Residues ◽  
Two Dimensional Gel Electrophoresis ◽  
Total Dna ◽  
Chromatin Proteins

Extraction of chicken reticulocyte and erythrocyte chromatins with 2 M NaCl yields a small fraction (about 5%) of the total DNA which is very tightly bound to a class of nonhistone chromatin proteins (DNA–P). This DNA fraction has previously been shown to be significantly enriched in active gene sequences. The proteins associated with reticulocyte and erythrocyte DNA–P were analyzed by two-dimensional gel electrophoresis. Reticulocyte DNA–P yield predominantly three major proteins, designated G1, G2, and G3 with relative masses of 80 000, 50 000, and 58 000, respectively. Erythrocyte DNA–P show only two proteins which appear to be similar to the reticulocyte G1 and G2 proteins, except in much reduced quantities as revealed by two-dimensional polyacrylamide gel electrophoresis. Amino acid analysis of the three reticulocyte proteins revealed that the ratio of acidic to basic amino acid residues increased in the order G1 < G2 < G3, while the respective isoelectric points also increased in that order.

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