INTERLEUKIN 1 INDUCES PROSTACYCLIN-DEPENDENT INCREASES IN CYCLIC AMP PRODUCTION AND DOES NOT AFFECT CYCLIC GMP PRODUCTION IN HUMAN VASCULAR SMOOTH MUSCLE CELLS.

Cytokine ◽  
1995 ◽  
Vol 7 (5) ◽  
pp. 417-426 ◽  
Author(s):  
Debbie Beasley ◽  
Mary E. McGuiggin
Keyword(s):  
Smooth Muscle ◽  
Cyclic Amp ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Cyclic Gmp ◽  
Interleukin 1 ◽  
Muscle Cells ◽  
Gmp Production

scholarly journals Agents which increase cyclic AMP have diverse effects on low-density-lipoprotein-receptor function in human vascular smooth-muscle cells and skin fibroblasts

1990 ◽  
Vol 267 (3) ◽  
pp. 607-614 ◽  
Author(s):  
A Middleton ◽  
B Middleton
Keyword(s):  
Smooth Muscle ◽  
Cyclic Amp ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Ldl Receptor ◽  
Muscle Cells ◽  
Skin Fibroblasts ◽  
Receptor Expression ◽  
Low Density

Receptor-mediated binding and metabolism of low-density lipoproteins (LDL) in cultured human vascular smooth-muscle cells and skin fibroblasts are altered by increased cellular cyclic AMP concentrations. However, the LDL receptor does not respond to changes in cyclic AMP concentration in a simple manner. The activation of adenylate cyclase with forskolin, or the addition of membrane-permeant cyclic AMP analogues, initially decreases the expression of the LDL receptor, but is followed by a substantial increase in receptor expression after 24 h. This increase does not occur in the presence of inhibitors of RNA or protein synthesis, and is due to doubling of the Bmax. of the LDL receptor, without alteration of its affinity for LDL. By contrast, elevation of cyclic AMP concentration by inhibition of phosphodiesterases results in decreased receptor expression throughout the 24 h period. These two response patterns are reproducible phenomena, consistently observed in low-passaged cells derived from seven unrelated individuals.

scholarly journals Endotoxin stimulated cytokine production in rat vascular smooth muscle cells

2001 ◽  
Vol 281 (2) ◽  
pp. H661-H668 ◽  
Author(s):  
Kristina Detmer ◽  
Zhongbiao Wang ◽  
Debra Warejcka ◽  
Sandra K. Leeper-Woodford ◽  
Walter H. Newman
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Target Genes ◽  
Interleukin 1 ◽  
Muscle Cells ◽  
Nuclear Factor Κb ◽  
Tnf Α

Because inflammatory processes may promote the development of atherosclerosis, we examined the activation of cytokine genes in rat vascular smooth muscle cells in vitro after treatment with bacterial lipopolysaccharide (LPS). Interleukin-1 (IL-1), IL-6 and tumor necrosis factor-α (TNF-α) mRNA increased in response to LPS. Activation of nuclear factor-κB (NF-κB) presumably results in NF-κB binding to regulatory regions of target genes and activating transcription. We therefore compared the kinetics of NF-κB activation, cytokine message production, and TNF-α secretion. Maximum active NF-κB was found at 30 min after the addition of LPS and decreased thereafter. Increased IL-6 mRNA was detected at 30 min, increased TNF-α mRNA at 60 min, and increased IL-1 mRNA at 120 min. Secretion of TNF-α was dependent on LPS concentration and was first detected 120 min after LPS addition. Aspirin, which has been shown to inhibit NF-κB activation and cytokine secretion in other cell types, did not inhibit NF-κB activation or TNF-α secretion. However, aspirin reduced the amount of both TNF-α and IL-6 mRNA present 30 min after LPS addition by half ( P < 0.05).

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