Haemonchus contortus: Identification of Proteases with Diverse Characteristics in Adult Worm Excretory-Secretory Products

1993 ◽  
Vol 77 (3) ◽  
pp. 362-371 ◽  
Author(s):  
F.N. Karanu ◽  
F.R. Rurangirwa ◽  
T.C. Mcguire ◽  
D.P. Jasmer
Keyword(s):  
Adult Worm ◽  
Haemonchus Contortus ◽  
Secretory Products

HcFAR, a functional inhibitor of goat TGF-β1 identified from excretory and secretory products of Haemonchus contortus

2020 ◽  
Vol 286 ◽  
pp. 109236
Author(s):  
Yongqian Bu ◽  
Man Cao ◽  
Xiaowei Tian ◽  
Mingmin Lu ◽  
Junjie Li ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Secretory Products ◽  
Tgf Β1

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 351-357 ◽  
Author(s):  
H. D. F. H. Schallig ◽  
M. A. W. van Leeuwen ◽  
W. M. L. Hendrikx
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
The Other ◽  
Proliferation Index ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Molecular Weights ◽  
Secretory Products

SUMMARYThe excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

scholarly journals Excretory-Secretory Products from Hookworm L3and Adult Worms Suppress Proinflammatory Cytokines in Infected Individuals

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Stefan Michael Geiger ◽  
Ricardo Toshio Fujiwara ◽  
Paula Albuquerque Freitas ◽  
Cristiano Lara Massara ◽  
Omar dos Santos Carvalho ◽  
...  
Keyword(s):  
Adult Worm ◽  
Proliferative Response ◽  
Mononuclear Cells ◽  
Chronic Phase ◽  
Larval Survival ◽  
Peripheral Blood Mononuclear ◽  
Third Stage ◽  
Secretory Products ◽  
Negative Controls ◽  
Necrosis Factor

We compared the effects of larval and adult worm excretory-secretory (ES) products from hookworm on the proliferative responses and cytokine secretion in peripheral blood mononuclear cells (PBMCs) from hookwormpatients and egg-negative, nonendemic controls. When compared with negative controls, mitogen-stimulated PBMC from hookworm-infected individuals showed a significantly reduced proliferative response when adult worm ES antigen was added to the cultures. Furthermore, in hookworm-infected individuals a significant downmodulation of inflammatory interleukin (IL)-6 and tumor necrosis factor (TNF)-αsecretion resulted when PBMCs were stimulated with mitogen in combination with larval or adult worm ES. Both, interferon (IFN)-γand IL-10 secretion were significantly lower in stimulated PBMC from infected individuals; however the IFN-γ/IL-10 ratio was much lower in hookworm-infected patients. Comparable effects, although at lower concentrations, were achieved when PBMCs from both groups were incubated with living hookworm third-stage larvae. We suggest that hookworm ES products downmodulate proliferative responses and inflammation during the chronic phase of the disease and facilitate early larval survival or adult worm persistence in the gut.

scholarly journals Vaccination against the nematode Haemonchus contortus with a thiol-binding fraction from the excretory/secretory products (ES)

Vaccine ◽  
2004 ◽  
Vol 22 (5-6) ◽  
pp. 618-628 ◽  
Author(s):  
N. Bakker ◽  
L. Vervelde ◽  
K. Kanobana ◽  
D.P. Knox ◽  
A.W.C.A. Cornelissen ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Secretory Products ◽  
Thiol Binding

Effects of excretory/secretory products of Haemonchus contortus on cell vacuolation

2005 ◽  
Vol 96 (5) ◽  
pp. 290-295 ◽  
Author(s):  
Alexandra Huber ◽  
Heinrich Prosl ◽  
Anja Joachim ◽  
Heather V Simpson ◽  
Kevin C Pedley
Keyword(s):  
Haemonchus Contortus ◽  
Secretory Products

scholarly journals HcTTR: a novel antagonist against goat interleukin 4 derived from the excretory and secretory products of Haemonchus contortus

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
XiaoWei Tian ◽  
MingMin Lu ◽  
WenJuan Wang ◽  
CaiWen Jia ◽  
Ehsan Muhammad ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Interleukin 4 ◽  
Secretory Products

scholarly journals Immunomodulatory dynamics of excretory and secretory products on Th9 immune response during Haemonchus contortus infection in goat

2020 ◽  
Vol 14 (4) ◽  
pp. e0008218 ◽  
Author(s):  
Muhammad Ali Memon ◽  
Muhammad Ali-ul-Husnain Naqvi ◽  
Huang Xin ◽  
Liang Meng ◽  
Muhammad Waqqas Hasan ◽  
...  
Keyword(s):  
Immune Response ◽  
Haemonchus Contortus ◽  
Secretory Products

scholarly journals Chemical constituents of Calotropis procera latex and ultrastructural effects on Haemonchus contortus

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Géssica Soares Cavalcante ◽  
Selene Maia de Morais ◽  
Weibson Paz Pinheiro André ◽  
José Vilemar de Araújo-Filho ◽  
Celli Rodrigues Muniz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Adult Worm ◽  
Protein Content ◽  
Haemonchus Contortus ◽  
High Performance ◽  
Chemical Constituents ◽  
Calotropis Procera

Abstract This study aimed to evaluate the anthelmintic and ultrastructural effects of Calotropis procera latex on Haemonchus contortus. C. procera latex was twice centrifuged at 10,000×g and dialyzed to obtain a fraction rich in proteins, named LP (latex protein), and at 3,000 rpm to obtain a fraction rich in secondary metabolites, named LNP (latex non-protein). Specimens of H. contortus exposed to LNP, LP and PBS in the Adult Worm Motility Test (AWMT) were submitted to scanning (SEM) and transmission (TEM) electron microscopy to verify changes in their ultrastructure. Phytochemical tests in the LNP indicated the presence of phenols, steroids, alkaloids and cardenolides. High-Performance Liquid Chromatography (HPLC) characterized the presence of the compounds gallic acid and quercetin in the LNP. The protein content in the LP was 43.1 ± 1.1 mg/mL and 7.7 ± 0.3 mg/mL in LNP. In AWMT, LNP and LP inhibited the motility of 100% of the nematodes, with LNP being more effective than LP and ivermectin more effective than both (p <0.05). Cuticle changes were observed by SEM and TEM in nematodes treated with LP and LNP. Calotropis procera latex has anthelmintic effects against H. contortus, causing damage to its cuticle and other alterations in its ultrastructure.

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