Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

2018 ◽  
pp. 201-210 ◽  
Author(s):  
Ehsan Taghiabadi ◽  
Bahareh Beiki ◽  
Nasser Aghdami ◽  
Amir Bajouri
Keyword(s):  
Tissue Engineering ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Adipose Derived Stromal Cells

scholarly journals Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 606
Author(s):  
Dilcele Silva Moreira Dziedzic ◽  
Bassam Felipe Mogharbel ◽  
Ana Carolina Irioda ◽  
Priscila Elias Ferreira Stricker ◽  
Maiara Carolina Perussolo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Cell Attachment ◽  
Periodontal Regeneration ◽  
Human Amniotic Membrane ◽  
Micro Computed Tomography ◽  
Adipose Derived Stromal Cells

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

scholarly journals Amniotic membrane and its epithelial and mesenchymal stem cells as an appropriate source for skin tissue engineering and regenerative medicine

2018 ◽  
Vol 46 (sup2) ◽  
pp. 431-440 ◽  
Author(s):  
Behrouz Farhadihosseinabadi ◽  
Mehrdad Farahani ◽  
Tahereh Tayebi ◽  
Ameneh Jafari ◽  
Felor Biniazan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Amniotic Membrane ◽  
Skin Tissue ◽  
Skin Tissue Engineering

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

Optimization and evaluation of ciprofloxacin-loaded collagen/chitosan scaffolds for skin tissue engineering

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Satyavrat Tripathi ◽  
Bhisham Narayan Singh ◽  
Divakar Singh ◽  
Gaurav kumar ◽  
Pradeep Srivastava
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Chitosan Scaffolds

Electrospun Skin Tissue Engineering Scaffolds Based on Polycaprolactone/Hyaluronic Acid/L-ascorbic Acid

2021 ◽  
Vol 22 (1) ◽  
pp. 19-29
Author(s):  
Mahsa Janmohammadi ◽  
Mohammad Sadegh Nourbakhsh ◽  
Shahin Bonakdar
Keyword(s):  
Tissue Engineering ◽  
Ascorbic Acid ◽  
Hyaluronic Acid ◽  
Skin Tissue ◽  
Tissue Engineering Scaffolds ◽  
Skin Tissue Engineering

Biomaterials in skin tissue engineering

2021 ◽  
pp. 1-19
Author(s):  
Chao Wei ◽  
Yihua Feng ◽  
Dezhao Che ◽  
Jiahui Zhang ◽  
Xuan Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering

scholarly journals 075 Generation and quantification of oxidized squalene to develop an acne testing model in vitro based on skin tissue engineering

2016 ◽  
Vol 136 (9) ◽  
pp. S173 ◽  
Author(s):  
N. Esselin ◽  
C. Capallere ◽  
C. Meyrignac ◽  
C. Plaza ◽  
C. Coquet ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Testing Model ◽  
Skin Tissue Engineering
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