scholarly journals Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 606
Author(s):  
Dilcele Silva Moreira Dziedzic ◽  
Bassam Felipe Mogharbel ◽  
Ana Carolina Irioda ◽  
Priscila Elias Ferreira Stricker ◽  
Maiara Carolina Perussolo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Cell Attachment ◽  
Periodontal Regeneration ◽  
Human Amniotic Membrane ◽  
Micro Computed Tomography ◽  
Adipose Derived Stromal Cells

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

Demineralized and decellularized bone extracellular matrix incorporated electrospun nanofibrous scaffold for bone regeneration

2021 ◽  
Author(s):  
Chanjuan Dong ◽  
Fangyu Qiao ◽  
Guobao Chen ◽  
Yonggang Lv
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Nanofibrous Scaffold ◽  
Proliferation And Differentiation

The extracellular matrix (ECM)-based materials has been employed as scaffolds for bone tissue engineering, providing a suitable microenvironment that possesses biophysical and biochemical cues for cell attachment, proliferation and differentiation....

Selective Laser Sintering of Polycaprolactone Bone Tissue Engineering Scaffolds

2004 ◽  
Vol 845 ◽  
Author(s):  
Brock Partee ◽  
Scott J. Hollister ◽  
Suman Das
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Selective Laser Sintering ◽  
Laser Sintering ◽  
Engineering Practice ◽  
Micro Computed Tomography ◽  
Tissue Engineering Scaffolds ◽  
Differentiation And Proliferation

ABSTRACTPresent tissue engineering practice requires porous, bioresorbable scaffolds to serve as temporary 3D templates to guide cell attachment, differentiation, and proliferation. Recent research suggests that scaffold material and internal architecture significantly influence regenerate tissue structure and function. However, lack of versatile biomaterials processing methods have slowed progress towards fully testing these findings. Our research investigates using selective laser sintering (SLS) to fabricate bone tissue engineering scaffolds. Using SLS, we have fabricated polycaprolactone (PCL) and polycaprolactone/tri-calcium phosphate composite scaffolds. We report on scaffold design and fabrication, mechanical property measurements, and structural characterization via optical microscopy and micro-computed tomography.

scholarly journals Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research

2015 ◽  
Vol 2 (11) ◽  
pp. 150496 ◽  
Author(s):  
Fabian Westhauser ◽  
Christian Weis ◽  
Melanie Hoellig ◽  
Tyler Swing ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Characteristic Parameter ◽  
Micro Computed Tomography ◽  
Mineral Density ◽  
Independent Analysis ◽  
Scaffold Properties

Bone tissue engineering and bone scaffold development represent two challenging fields in orthopaedic research. Micro-computed tomography (mCT) allows non-invasive measurement of these scaffolds’ properties in vivo . However, the lack of standardized mCT analysis protocols and, therefore, the protocols’ user-dependency make interpretation of the reported results difficult. To overcome these issues in scaffold research, we introduce the Heidelberg-mCT-Analyzer. For evaluation of our technique, we built 10 bone-inducing scaffolds, which underwent mCT acquisition before ectopic implantation (T0) in mice, and at explantation eight weeks thereafter (T1). The scaffolds’ three-dimensional reconstructions were automatically segmented using fuzzy clustering with fully automatic level-setting. The scaffold itself and its pores were then evaluated for T0 and T1. Analysing the scaffolds’ characteristic parameter set with our quantification method showed bone formation over time. We were able to demonstrate that our algorithm obtained the same results for basic scaffold parameters (e.g. scaffold volume, pore number and pore volume) as other established analysis methods. Furthermore, our algorithm was able to analyse more complex parameters, such as pore size range, tissue mineral density and scaffold surface. Our imaging and post-processing strategy enables standardized and user-independent analysis of scaffold properties, and therefore is able to improve the quantitative evaluations of scaffold-associated bone tissue-engineering projects.

Graft Materials and Bone Marrow Stromal Cells in Bone Tissue Engineering

2011 ◽  
Vol 26 (8) ◽  
pp. 1035-1049 ◽  
Author(s):  
Federico Foschi ◽  
Enrico Conserva ◽  
Paolo Pera ◽  
Barbara Canciani ◽  
Ranieri Cancedda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells
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