Validation of the Media Fill Method for Solid Tissue Processing in Good Manufacturing Practice–Compliant Cell Production

2020 ◽  
Author(s):  
Olga Nehir Öztel ◽  
Nurullah Aydoğdu ◽  
Erdal Karaöz
Keyword(s):  
Good Manufacturing Practice ◽  
Cell Production ◽  
Solid Tissue ◽  
Tissue Processing ◽  
The Media

scholarly journals Inactivated human platelet lysate with psoralen: a new perspective for mesenchymal stromal cell production in Good Manufacturing Practice conditions

Cytotherapy ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 750-763 ◽  
Author(s):  
Sara Castiglia ◽  
Katia Mareschi ◽  
Luciana Labanca ◽  
Graziella Lucania ◽  
Marco Leone ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Human Platelet ◽  
Good Manufacturing Practice ◽  
Platelet Lysate ◽  
Cell Production ◽  
Human Platelet Lysate ◽  
New Perspective

scholarly journals GMP compliant isolation of mucosal epithelial cells and fibroblasts from biopsy samples for clinical tissue engineering

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Angela Tait ◽  
Toby Proctor ◽  
Nick J. I. Hamilton ◽  
Martin A. Birchall ◽  
Mark W. Lowdell
Keyword(s):  
Epithelial Cells ◽  
Gold Standard ◽  
Good Manufacturing Practice ◽  
Raw Material ◽  
Stem Cell Population ◽  
Upper Aerodigestive Tract ◽  
Cell Sheets ◽  
Primary Fibroblast ◽  
Serum Supplement ◽  
The Media

AbstractEngineered epithelial cell sheets for clinical replacement of non-functional upper aerodigestive tract mucosa are regulated as medicinal products and should be manufactured to the standards of good manufacturing practice (GMP). The current gold standard for growth of epithelial cells for research utilises growth arrested murine 3T3 J2 feeder layers, which are not available for use as a GMP compliant raw material. Using porcine mucosal tissue, we demonstrate a new method for obtaining and growing non-keratinised squamous epithelial cells and fibroblast cells from a single biopsy, replacing the 3T3 J2 with a growth arrested primary fibroblast feeder layer and using pooled Human Platelet lysate (HPL) as the media serum supplement to replace foetal bovine serum (FBS). The initial isolation of the cells was semi-automated using an Octodissociator and the resultant cell suspension cryopreservation for future use. When compared to the gold standard of 3T3 J2 and FBS containing medium there was no reduction in growth, viability, stem cell population or ability to differentiate to mature epithelial cells. Furthermore, this method was replicated with Human buccal tissue, providing cells of sufficient quality and number to create a tissue engineered sheet.

Adam Briggs and Paul Cobley (eds.), The Media: An Introduction,Harlow: Longman, 1998, £16.99 pbk, xii+449 pp. (ISSN: 0-5822-7798-1).

Sociology ◽  
1998 ◽  
Vol 32 (4) ◽  
pp. 881-900
Author(s):  
Sara O'Sullivan
Keyword(s):  
The Media

Using the scanning electron microscope for failure analysis of high density magnetic media

1987 ◽  
Vol 45 ◽  
pp. 392-393
Author(s):  
Evelyn R. Ackerman ◽  
Gary D. Burnett
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Failure Analysis ◽  
High Density ◽  
Magnetic Media ◽  
Specific Data ◽  
Retrieval Systems ◽  
Operating Environments ◽  
The Media ◽  
Scanning Electron

Advancements in state of the art high density Head/Disk retrieval systems has increased the demand for sophisticated failure analysis methods. From 1968 to 1974 the emphasis was on the number of tracks per inch. (TPI) ranging from 100 to 400 as summarized in Table 1. This emphasis shifted with the increase in densities to include the number of bits per inch (BPI). A bit is formed by magnetizing the Fe203 particles of the media in one direction and allowing magnetic heads to recognize specific data patterns. From 1977 to 1986 the tracks per inch increased from 470 to 1400 corresponding to an increase from 6300 to 10,800 bits per inch respectively. Due to the reduction in the bit and track sizes, build and operating environments of systems have become critical factors in media reliability.Using the Ferrofluid pattern developing technique, the scanning electron microscope can be a valuable diagnostic tool in the examination of failure sites on disks.

Spreading the Word: Raise Your Profession's Profile With Local Angles on National News

ASHA Leader ◽  
2013 ◽  
Vol 18 (2) ◽  
Keyword(s):  
Know How ◽  
The Media

How to use your local know-how to get the media to pay attention.

Democracy and the Media

2000 ◽  
Keyword(s):  
The Media

Rate of red cell production in two nephrectomized patients

1968 ◽  
Vol 122 (3) ◽  
pp. 230-235 ◽  
Author(s):  
A. J. Erslev
Keyword(s):  
Red Cell ◽  
Cell Production

Suicide and the Media

Crisis ◽  
2001 ◽  
Vol 22 (4) ◽  
pp. 163-169 ◽  
Author(s):  
R. Warwick Blood ◽  
Jane Pirkis
Keyword(s):  
Social Learning Theory ◽  
The Body ◽  
Media Content ◽  
Causal Association ◽  
Media Messages ◽  
Film And Television ◽  
Media Portrayal ◽  
The Media ◽  
Actual Suicide ◽  
Multiple Meanings

Summary: The body of evidence suggests that there is a causal association between nonfictional media reporting of suicide (in newspapers, on television, and in books) and actual suicide, and that there may be one between fictional media portrayal (in film and television, in music, and in plays) and actual suicide. This finding has been explained by social learning theory. The majority of studies upon which this finding is based fall into the media “effects tradition,” which has been criticized for its positivist-like approach that fails to take into account of media content or the capacity of audiences to make meaning out of messages. A cultural studies approach that relies on discourse and frame analyses to explore meanings, and that qualitatively examines the multiple meanings that audiences give to media messages, could complement the effects tradition. Together, these approaches have the potential to clarify the notion of what constitutes responsible reporting of suicide, and to broaden the framework for evaluating media performance.

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