Microbial Biomass and Community Composition Analysis Using Phospholipid Fatty Acids

2016 ◽  
pp. 65-76 ◽  
Author(s):  
Aaron D. Peacock ◽  
David C. White
Keyword(s):  
Fatty Acids ◽  
Microbial Biomass ◽  
Community Composition ◽  
Phospholipid Fatty Acids ◽  
Composition Analysis

Effects of naphthalene on soil microbial biomass, microbial abundance, and microbial phospholipid fatty acids in a subalpine forest of western Sichuan

2017 ◽  
Vol 37 (23) ◽  
Author(s):  
蓝丽英 LAN Liying ◽  
寥蓉 LIAO Rong ◽  
杨万勤 YANG Wanqin ◽  
吴福忠 WU Fuzhong ◽  
杨帆 YANG Fan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microbial Biomass ◽  
Soil Microbial Biomass ◽  
Phospholipid Fatty Acids ◽  
Subalpine Forest ◽  
Microbial Abundance ◽  
Western Sichuan ◽  
Soil Microbial

Extraction and methylation parameters of phospholipid fatty acid analysis and their effect on total yield and community structure

2021 ◽  
Author(s):  
Guido Wiesenberg ◽  
Cyrill Zosso
Keyword(s):  
Fatty Acids ◽  
Community Composition ◽  
Cell Walls ◽  
Phospholipid Fatty Acid ◽  
Total Yield ◽  
Fatty Acid Analysis ◽  
Phospholipid Fatty Acids ◽  
Acid Catalyzed ◽  
Relative Abundances ◽  
Microbial Groups

<p>Phospholipid fatty acids are membrane compounds of microbial cell walls and the structure of individual compounds is indicative for specific microbial groups. The extraction and analysis of phospholipid fatty acids in soils improved our understanding of factors driving microbial abundance and community composition. Despite the wide application of this method, important pitfalls persist which impede comparability of PLFA results between studies.</p><p>Here, we show that there was an effect of freeze-drying on the community composition. However, compared to the effect of using of old extraction solution (4 weeks) and two different methylation procedures, this effect seems negligible. Using old extraction solution, the overall yield of PLFA was 12% lower and and we observed significant differences in the relative abundances of functional microbial groups. But most importantly, base catalyzed methylation yielded 35% less PLFA compared to acid catalyzed methylation and the relative abundances of all microbial groups were completely different. Our results show that it is crucial to keep the analytical parameters constant to capture subtle treatment effects and that especially the use of different methylation methods prevents comparability between studies.</p>

By-products as an amendment of a mine soil: effects on microbial biomass determined using phospholipid fatty acids

2018 ◽  
Vol 8 ◽  
Author(s):  
Vanesa Santás-Miguel ◽  
Laura Cutillas-Barreiro ◽  
Juan Carlos Nóvoa-Muñoz ◽  
Manuel Arias-Estévez ◽  
Montserrat Díaz-Raviña ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Community Structure ◽  
Microbial Biomass ◽  
Fungal Biomass ◽  
Phospholipid Fatty Acids ◽  
Pine Bark ◽  
Mine Soil ◽  
Mussel Shell ◽  
By Products

In the present work, the effect of two by-products (pine bark and crushed mussel shell) on microbial biomass and community structure was studied in a soil from a mine tailing located in a copper mine. In a laboratory experiment, different doses (0, 12, 24, 48, 96 and 192 Mg ha<sup>-1</sup>) of pine bark, crushed mussel shell or mixtures of both by-products were added to the soil. The amended soil samples were incubated for one year at 60% of water holding capacity, and then 33 phospholipid fatty acids (PLFAs) were extracted from these samples and quantified. The PLFAs concentrations were used for different microbial biomass estimations: total biomass, bacterial biomass, fungal biomass, gram-positive (G+) biomass and gram-negative (G-) biomass. The addition of crushed mussel had no significant effects on the total soil microbial biomass, either bacterial of fungal biomass. However, the addition of pine bark increased the total microbial biomass in the soil (up to 40%), mainly due to increases in the fungal biomass (it increased 1600%). No synergistic effects were observed when the soil was amended with both, pine bark and crushed mussel shell. The main community structure changes were due to the addition of pine bark to the soil, and were also due to modifications in fungal communities. Our results suggest that the microbial biomass was mainly limited in the mine soil by low organic matter concentrations, and therefore, practices increasing the amount of soil organic matter should be priorities for soil reclamation.

scholarly journals Interaction between Erythrocyte Phospholipid Fatty Acids Composition and Variants of Inflammation-Related Genes on Type 2 Diabetes

2015 ◽  
Vol 7 (4-6) ◽  
pp. 252-263 ◽  
Author(s):  
Kelei Li ◽  
Tao Huang ◽  
Ju-Sheng Zheng ◽  
Jianqin Sun ◽  
Yanqiu Chen ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Fatty Acids ◽  
Phospholipid Fatty Acids ◽  
Fatty Acids Composition

Effect of essential fatty acid depletion on tissue phospholipid fatty acids in spontaneously hypertensive and normotensive rats

Lipids ◽  
1989 ◽  
Vol 24 (7) ◽  
pp. 565-571 ◽  
Author(s):  
Yung-Sheng Huang ◽  
Dave E. Mills ◽  
Ron P. Ward ◽  
David F. Horrobin ◽  
Valerie A. Simmons
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Fatty Acid ◽  
Phospholipid Fatty Acids ◽  
Spontaneously Hypertensive ◽  
Tissue Phospholipid

Serum Phospholipid Fatty Acids in Benign Breast Tumor and Breast Cancer

2009 ◽  
Vol 42 (3) ◽  
pp. 213
Author(s):  
Eugene Shim ◽  
Sei Hyun Ahn ◽  
You-Jeong Hwang ◽  
Yang Cha Lee-Kim
Keyword(s):  
Breast Cancer ◽  
Fatty Acids ◽  
Breast Tumor ◽  
Phospholipid Fatty Acids ◽  
Serum Phospholipid ◽  
Benign Breast Tumor
Sign in / Sign up

Export Citation Format

Share Document