Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

2020 ◽  
pp. 145-151
Author(s):  
Anuk Indraratna ◽  
David M. P. De Oliveira ◽  
Lauren E. Hartley-Tassell ◽  
Christopher J. Day ◽  
Mark J. Walker ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Microarray Analysis ◽  
High Throughput ◽  
Whole Cells ◽  
Glycan Microarray ◽  
Group A

High-Throughput Carbohydrate Microarray Analysis of 24 Lectins

2006 ◽  
Vol 45 (22) ◽  
pp. 3607-3610 ◽  
Author(s):  
Joseph C. Manimala ◽  
Timothy A. Roach ◽  
Zhitao Li ◽  
Jeffrey C. Gildersleeve
Keyword(s):  
Microarray Analysis ◽  
High Throughput

scholarly journals High-Throughput Tissue Microarray Analysis to Evaluate Genes Uncovered by cDNA Microarray Screening in Renal Cell Carcinoma

1999 ◽  
Vol 154 (4) ◽  
pp. 981-986 ◽  
Author(s):  
Holger Moch ◽  
Peter Schraml ◽  
Lukas Bubendorf ◽  
Martina Mirlacher ◽  
Juha Kononen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tissue Microarray ◽  
Microarray Analysis ◽  
Cdna Microarray ◽  
High Throughput ◽  
Renal Cell ◽  
Microarray Screening ◽  
Tissue Microarray Analysis

High-throughput ion exchange purification of positively charged recombinant protein in the presence of negatively charged dextran sulfate

2014 ◽  
Vol 30 (2) ◽  
pp. 516-520 ◽  
Author(s):  
Lam Raga A. Markely ◽  
Lutfiye Kurt ◽  
Janet Lau ◽  
Sarthak Mane ◽  
Bing Guan ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Recombinant Protein ◽  
High Throughput ◽  
Dextran Sulfate ◽  
Positively Charged

Glycan microarray analysis of Candida glabrata adhesin ligand specificity

2008 ◽  
Vol 68 (3) ◽  
pp. 547-559 ◽  
Author(s):  
Margaret L. Zupancic ◽  
Matthew Frieman ◽  
David Smith ◽  
Richard A. Alvarez ◽  
Richard D. Cummings ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Candida Glabrata ◽  
Ligand Specificity ◽  
Glycan Microarray

scholarly journals Streptococcal-induced cell-mediated-immune destruction of cardiac myofibers in vitro.

1977 ◽  
Vol 146 (2) ◽  
pp. 344-360 ◽  
Author(s):  
L C Yang ◽  
P R Soprey ◽  
M K Wittner ◽  
E N Fox
Keyword(s):  
Antigenic Determinants ◽  
Target Cells ◽  
Heart Cells ◽  
Guinea Pig Heart ◽  
Whole Cells ◽  
Group A ◽  
Streptococcal Antigens ◽  
Lymphocyte Cytotoxicity

We have demonstrated that T lymphocytes from the spleens of adult guinea pigs sensitized to group A streptococcal antigens are cytotoxic for cultured fetal guinea pig heart cells. Lymphocyte cytotoxicity, measured by 51Cr release from target cells, was stimulated by sensitization in vivo with group A whole cells, cell walls, and purified protoplast membranes emulsified with complete Freund's adjuvant (CFA). Sensitization with group C streptococcal antigens in CFA or CFA alone produced lymphocytes with little or no specific cytotoxic activity. Target cells of cultured fetal skeletal muscle, liver, or skin were relatively refractory to effector cell cytotoxicity. The presence of antigenic determinants on the membranes of cultured myofibers, cross-reacting with group A streptococcal cellular antigens, was confirmed by immunofluorescence. These data are discussed in terms of a model for poststreptococcal rheumatic myocarditis in which cell-mediated autoimmune mechanisms may participate.

scholarly journals High throughput tissue microarray analysis of FHIT expression in diffuse large cell B-cell lymphoma from Saudi Arabia

2006 ◽  
Vol 19 (8) ◽  
pp. 1124-1129 ◽  
Author(s):  
Khawla Al Kuraya ◽  
Abdul Khalid Siraj ◽  
Prashant Bavi ◽  
Naif Al-Jomah ◽  
Hassan El-Solh ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
B Cell ◽  
Tissue Microarray ◽  
Microarray Analysis ◽  
High Throughput ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large Cell ◽  
Tissue Microarray Analysis

scholarly journals The prosthetic group of methanol dehydrogenase. Purification and some of its properties

1980 ◽  
Vol 187 (1) ◽  
pp. 221-226 ◽  
Author(s):  
J A Duine ◽  
J Frank
Keyword(s):  
Methanol Dehydrogenase ◽  
Water Soluble ◽  
Soluble Nitrogen ◽  
Prosthetic Group ◽  
Whole Cells ◽  
Group A

Methanol dehydrogenases isolated from bacteria belonging to different classes of methylotrophs contain the same prosthetic group. A procedure for its purification from whole cells is given. The reduced and oxidized form of the enzyme from Hyphomicrobium X and those of the isolated group are compared and it is concluded that the latter indeed functions in the enzyme. Further evidence is presented that the prosthetic group is not a pterine or lumazine derivative, but a water-soluble nitrogen-containing quinone.

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