Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins

2020 ◽  
pp. 443-462
Author(s):  
David Roujol ◽  
Laurent Hoffmann ◽  
Hélène San Clemente ◽  
Corinne Schmitt-Keichinger ◽  
Christophe Ritzenthaler ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Biology ◽  
Plant Cell Wall ◽  
Bona Fide

scholarly journals Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Brian T DeVree ◽  
Lisa M Steiner ◽  
Sylwia Głazowska ◽  
Felix Ruhnow ◽  
Klaus Herburger ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Plant Cell Wall ◽  
Imaging Techniques ◽  
Molecular Probes ◽  
Comprehensive Overview ◽  
Biotechnology Research ◽  
High Level

AbstractPlant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different classes of polysaccharides, proteoglycans, and aromatic polymers and are one of the most complex structures in Nature. One of the most challenging tasks of cell biology and biomass biotechnology research is to image the structure and organization of this complex matrix, as well as to visualize the compartmentalized, multiplayer biosynthetic machineries that build the elaborate cell wall architecture. Better knowledge of the plant cells, cell walls, and whole tissue is essential for bioengineering efforts and for designing efficient strategies of industrial deconstruction of the cell wall-derived biomass and its saccharification. Cell wall-directed molecular probes and analysis by light microscopy, which is capable of imaging with a high level of specificity, little sample processing, and often in real time, are important tools to understand cell wall assemblies. This review provides a comprehensive overview about the possibilities for fluorescence label-based imaging techniques and a variety of probing methods, discussing both well-established and emerging tools. Examples of applications of these tools are provided. We also list and discuss the advantages and limitations of the methods. Specifically, we elaborate on what are the most important considerations when applying a particular technique for plants, the potential for future development, and how the plant cell wall field might be inspired by advances in the biomedical and general cell biology fields.

A participation of weak-bound with plant cell wall peroxidases in ring rot tolerance of wild Mexican species and cultural varieties of potato

2014 ◽  
pp. 103-108
Author(s):  
M.A. Zhivetiev ◽  
◽  
A.V. Papkina ◽  
I.A. Graskova ◽  
V.K. Voinikov ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Ring Rot ◽  
Mexican Species

Ultrasound Assisted Solid-Liquid Extraction Devices - Harnessing Active Ingredients from Plant materials – Overview and Analysis

2020 ◽  
Vol 13 ◽  
Author(s):  
Venkatasubramanian Sivakumar
Keyword(s):  
Mass Transfer ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Liquid Extraction ◽  
Active Ingredients ◽  
Plant Materials ◽  
Ultrasound Assisted ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Background: In the growing environmental concern use of natural products, efficient processes and devices are necessary. Solid-Liquid extraction of active Ingredients from Plant materials is one of the important unit operations in Chemical Engineering and need to be enhanced. Objectives: Since, these active ingredients are firmly bound to the plant cell wall membrane, which pose mass-transfer resistance and need to get detached through the use of suitable process intensification tools such as ultrasound and suitable devices. Therefore, detailed analysis and review is essential on development made in this area through Publications and Patents. Hence, the present paper illustrates the development of ultrasound assisted device for solid-liquid extraction are presented in this paper. Methods: Advantages such as % Yield, Reduction in extraction time, use of ambient conditions, better process control, avoidance or minimizing multi stage extraction could be achieved due to the use of ultrasound in extraction as compared to conventional processes. Conclusions: Use of ultrasound to provide significant improvements in the extraction of Vegetable tannins, Natural dyes for application in Leather processing has been demonstrated and reported earlier. These enhancement could be possible through various effects of ultrasound such as better flow of solvents through micro-jet formation, mass transfer enhancement due to rupture of plant cell wall membranes through acoustic cavitation, better leaching due to micro-mixing and acoustic streaming effects. This approach would minimize material wastage; thereby, leading to eco-conservation of plant materials, which is very much essential for better environment. Hence, various methods and design for application of ultrasound assisted solid-liquid extractor device are necessary.

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