Field Emission Scanning Electron Microscopy in Cell Biology Featuring the Plant Cell Wall and Nuclear Envelope

2019 ◽  
pp. 343-362
Author(s):  
Martin W. Goldberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Nuclear Envelope ◽  
Field Emission ◽  
Plant Cell ◽  
Cell Biology ◽  
Plant Cell Wall ◽  
Scanning Electron

scholarly journals A Simple Technique For The Removal Of Plant Cell Protoplasm To Facilitate Scanning : Electron Microscopy Of Fungal Haustoria And Plant Cell Wall Features

2001 ◽  
Vol 9 (3) ◽  
pp. 14-15 ◽  
Author(s):  
B. A. Richardson ◽  
C. W. Mims
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Host Plants ◽  
Plant Cell Wall ◽  
Simple Technique ◽  
Plant Cells ◽  
Host Cell Wall ◽  
Scanning Electron

Several years ago Honegger (1985) described a simple technique for removing plant cell protoplasm in order to reveal details of interfaces between plant cells and fungal structures. This technique involves the use of Ariel a commercially available washing powder (Proctor and Gamble) containing a Bacillus substilis derived protease. We since have used this technique with excellent results to examine not only the morphology of fungal haustoria inside leaf cells of various host plants but also features of the inner surface of the host cell wall with scanning electron microscopy (SEM). Here we describe the procedure we have used to prepare samples for study and provide examples of the types of images we have obtained from our samples.

High-Resolution Field Emission Scanning Electron Microscopy (FESEM) Imaging of Cellulose Microfibril Organization in Plant Primary Cell Walls

2017 ◽  
Vol 23 (5) ◽  
pp. 1048-1054 ◽  
Author(s):  
Yunzhen Zheng ◽  
Daniel J. Cosgrove ◽  
Gang Ning
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
High Resolution ◽  
Field Emission ◽  
Cell Walls ◽  
Cellulose Microfibrils ◽  
Critical Point Drying ◽  
Sputter Coating ◽  
Scanning Electron

AbstractWe have used field emission scanning electron microscopy (FESEM) to study the high-resolution organization of cellulose microfibrils in onion epidermal cell walls. We frequently found that conventional “rule of thumb” conditions for imaging of biological samples did not yield high-resolution images of cellulose organization and often resulted in artifacts or distortions of cell wall structure. Here we detail our method of one-step fixation and dehydration with 100% ethanol, followed by critical point drying, ultrathin iridium (Ir) sputter coating (3 s), and FESEM imaging at a moderate accelerating voltage (10 kV) with an In-lens detector. We compare results obtained with our improved protocol with images obtained with samples processed by conventional aldehyde fixation, graded dehydration, sputter coating with Au, Au/Pd, or carbon, and low-voltage FESEM imaging. The results demonstrated that our protocol is simpler, causes little artifact, and is more suitable for high-resolution imaging of cell wall cellulose microfibrils whereas such imaging is very challenging by conventional methods.

scholarly journals Developments in application of light and scanning electron microscopy techniques for cell wall degradation studies

1996 ◽  
Vol 44 (4) ◽  
pp. 357-373
Author(s):  
F.M. Engels
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell Wall ◽  
Histochemical Staining ◽  
Plant Tissues ◽  
Cell Wall Degradation ◽  
Rumen Microorganisms ◽  
Technological Developments ◽  
Scanning Electron

The results of recent technological developments in light and scanning electron microscopy closely used for research on forage cell wall degradation in ruminants, are reviewed. The indigestibility of forages by rumen microorganisms used to be ascribed mainly to an overall presence of lignin in the plant material. However, early light microscopic observations without application of histochemical staining revealed that some leaf and stem tissues were degraded completely. The early use of lignin detecting dyes, such as acid phloroglucinol or safranin, in light microscopy made it possible to discriminate between lignified undegradable and unlignified degradable plant tissues. The introduction of the scanning electron microscope enabled a further discrimination between degradable and undegradable cell wall and cell wall layers in plant tissues. As a result of continuous improvement of the techniques used in microscopy, e.g. section to slide, mirror sectioning, microspectrophotometry and cryo-ultramilling, forage indigestibility can now be attributed to the specific deposition and location of cutin/suberin or lignin layers inside the plant cell wall. These structural layers form barriers hindering access of rumen microorganisms to degradable parts of the cell wall.

A New Image Processing Technique for STEM

1974 ◽  
Vol 32 ◽  
pp. 432-433
Author(s):  
Yasushi Kokubo ◽  
Hirotami Koike ◽  
Teruo Someya
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Scanning Electron Microscopy ◽  
Elastic Scattering ◽  
Field Emission ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Energy Analyzer ◽  
Scanning Microscope ◽  
Scanning Electron

One of the advantages of scanning electron microscopy is the capability for processing the image contrast, i.e., the image processing technique. Crewe et al were the first to apply this technique to a field emission scanning microscope and show images of individual atoms. They obtained a contrast which depended exclusively on the atomic numbers of specimen elements (Zcontrast), by displaying the images treated with the intensity ratio of elastically scattered to inelastically scattered electrons. The elastic scattering electrons were extracted by a solid detector and inelastic scattering electrons by an energy analyzer. We noted, however, that there is a possibility of the same contrast being obtained only by using an annular-type solid detector consisting of multiple concentric detector elements.

Defects of Platelet Function Recognized by X-Ray Imaging and Scanning Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 610-613
Author(s):  
M.G. Baldini ◽  
S. Morinaga ◽  
D. Minasian ◽  
R. Feder ◽  
D. Sayre ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Biology ◽  
Imaging Technique ◽  
Human Platelets ◽  
X Rays ◽  
X Ray ◽  
X Ray Imaging ◽  
Complex Phenomena ◽  
Scanning Electron

Contact X-ray imaging is presently developing as an important imaging technique in cell biology. Our recent studies on human platelets have demonstrated that the cytoskeleton of these cells contains photondense structures which can preferentially be imaged by soft X-ray imaging. Our present research has dealt with platelet activation, i.e., the complex phenomena which precede platelet appregation and are associated with profound changes in platelet cytoskeleton. Human platelets suspended in plasma were used. Whole cell mounts were fixed and dehydrated, then exposed to a stationary source of soft X-rays as previously described. Developed replicas and respective grids were studied by scanning electron microscopy (SEM).

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