Vaccine Based on Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System

2020 ◽  
pp. 153-160
Author(s):  
Yadira Pastor ◽  
Isaiah Ting ◽  
Melibea Berzosa ◽  
Juan M. Irache ◽  
Carlos Gamazo
Keyword(s):  
Outer Membrane ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Vaccine Delivery System

Outer Membrane Vesicles of Bordetella pertussis encapsulated into sodium alginate nanoparticles as novel vaccine delivery system

2021 ◽  
Vol 27 ◽  
Author(s):  
Abbas Rami ◽  
Fatemeh Kazemi-Lomedasht ◽  
Ali Mirjalili ◽  
Mojtaba Noofeli ◽  
Fereshteh Shahcheraghi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Western Blot ◽  
Outer Membrane ◽  
Sodium Alginate ◽  
Delivery System ◽  
Outer Membrane Proteins ◽  
Vaccine Delivery ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Vaccine Delivery System

Background: Outer membrane vesicles (OMVs) release from Gram-negative bacteria and are interesting alternatives that can replace those vaccines that contain naturally incorporated bacterial surface antigens, lipopolysaccharides (LPS) and outer membrane proteins (OMPs). Nanoparticles can be used to encapsulate vesicles for slow release and prevent macromolecular degradation. Objective: Therefore, encapsulation of OMVs of B. pertussis into sodium alginate nanoparticles was the main aim of the current study. Method: The OMVs of B. pertussis extracted and characterized by particle sizer, electron microscopy, SDSPAGE and Western blot assays. The extracted OMVs were encapsulated in sodium alginate nanoparticles (OMV-NP) using unique gelation process and injected into BALB/c mice. Immunogenicity indices such as different classes of antibodies and interleukins related to different T cell lines were evaluated in immunized mice by ELISA. The respiratory challenge was evaluated in the groups of mice. The existence of pertussis toxin (PTX), filamentous haemagglutinin (FHA) and PRN (pertactin) in B. pertussis OMVs was verified using SDS-PAGE and Western blot analysis. Results: TEM electron microscopy showed the size of these OMVs to be around 20-80 nm. The OMVs with appropriate quality were encapsulated into sodium alginate nanoparticles (OMV-NP), and the final size was about 500 nm after encapsulation. Immunity indices were significantly higher in the OMV-NP receiving group. In challenge tests, the OMV-NP vaccine was able to show the highest rate of lung clearance compared to the control groups (OMV and wPV) at the lowest injection dose. Conclusion: The results indicate the potential of OMV-NP as a novel vaccine delivery system.

scholarly journals Electroporation of outer membrane vesicles derived from Pseudomonas aeruginosa with gold nanoparticles

2019 ◽  
Vol 1 (12) ◽  
Author(s):  
Zeineb Ayed ◽  
Luana Cuvillier ◽  
Garima Dobhal ◽  
Renee V. Goreham
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Outer Membrane ◽  
Drug Delivery System ◽  
Delivery System ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Novel Drug Delivery System ◽  
Novel Drug

Abstract Since their discovery, extracellular vesicles have gained considerable scientific interest as a novel drug delivery system. In particular, outer membrane vesicles (OMVs) play a critical role in bacteria–bacteria communication and bacteria–host interactions by trafficking cell signalling biochemicals (i.e. DNA, RNA, proteins). Although previous studies have focused on the use of OMVs as vaccines, little work has been done on loading them with functional nanomaterials for drug delivery. We have developed a novel drug delivery system by loading OMVs with gold nanoparticles (AuNPs). AuNPs are versatile nanoparticles that have been extensively used in disease therapeutics. The particles were loaded into the vesicles via electroporation, which uses an electric pulse to create a short-lived electric field. The resulting capacitance on the membrane generates pores in the lipid bilayer of the OMVs allowing AuNPs (or any nanoparticle under 10 nm) inside the vesicles. Closure of the pores of the lipid membrane of the OMVs entraps the nanoparticles as cargo. Transmission electron microscopy was used to confirm the loading of AuNPs inside the OMVs and dynamic light scattering (DLS) and cryogenic scanning electron microscopy (cryo-SEM) verified the size and integrity of the OMVs. This is the first report to load nanoparticles into OMVs, demonstrating a potential method for drug delivery. Graphic abstract

scholarly journals Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles

2009 ◽  
Vol 1788 (10) ◽  
pp. 2150-2159 ◽  
Author(s):  
Sang-Hyun Kim ◽  
Keun-Su Kim ◽  
Sang-Rae Lee ◽  
Ekyune Kim ◽  
Myeong-Su Kim ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Vaccine Delivery ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Structural Modifications ◽  
Delivery Vehicles

Designer outer membrane vesicles as immunomodulatory systems – Reprogramming bacteria for vaccine delivery

2017 ◽  
Vol 114 ◽  
pp. 132-142 ◽  
Author(s):  
Yehou M.D. Gnopo ◽  
Hannah C. Watkins ◽  
Taylor C. Stevenson ◽  
Matthew P. DeLisa ◽  
David Putnam
Keyword(s):  
Outer Membrane ◽  
Vaccine Delivery ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

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