Synthesis and Biological Activity of the Immunomodulatory Site of Interleukin 1

1987 ◽  
pp. 251-258
Author(s):  
G. Antoni ◽  
R. Presentini ◽  
F. Perin ◽  
A. Tagliabue ◽  
P. Ghiara ◽  
...  
Keyword(s):  
Biological Activity ◽  
Interleukin 1

Biological activity of recombinant murine interleukin-6 in interleukin-1 T cell assays

1989 ◽  
Vol 120 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Takashi Suda ◽  
Philip Hodgkin ◽  
Frank Lee ◽  
Albert Zlotnik
Keyword(s):  
Biological Activity ◽  
T Cell ◽  
Interleukin 6 ◽  
Interleukin 1 ◽  
Cell Assays ◽  
T Cell Assays

Expression and biological activity of interleukin-1 receptors in mouse γ/δ thymocytes during ontogeny

1992 ◽  
Vol 22 (11) ◽  
pp. 2849-2854 ◽  
Author(s):  
Isabelle Liberman ◽  
Pierre Sarthou ◽  
Pierre-André Cazenave ◽  
Dominique Rueff-Juy
Keyword(s):  
Biological Activity ◽  
Interleukin 1

High-affinity binding of PDGF-AA and PDGF-BB to normal human osteoblastic cells and modulation by interleukin-1

1991 ◽  
Vol 261 (6) ◽  
pp. C980-C985 ◽  
Author(s):  
R. S. Gilardetti ◽  
M. S. Chaibi ◽  
J. Stroumza ◽  
S. R. Williams ◽  
H. N. Antoniades ◽  
...  
Keyword(s):  
Biological Activity ◽  
Growth Factors ◽  
Binding Sites ◽  
Thymidine Incorporation ◽  
Interleukin 1 ◽  
Biologically Active ◽  
Osteoblastic Cells ◽  
High Affinity ◽  
Normal Human ◽  
Human Osteoblastic Cells

Bone has the capacity for repair and regeneration. The repair process is thought to be locally regulated by growth factors. One of the growth factors that potentially plays a significant role in these processes is platelet-derived growth factor (PDGF). Two different PDGF genes have been identified, PDGF-A and PDGF-B, whose gene products give rise to biologically active dimers. We now report that PDGF-AA and PDGF-BB exhibit saturable binding to normal human osteoblastic cells. By Scatchard analysis we estimate that there are approximately 43,000 PDGF-AA binding sites per cell, with a dissociation constant (Kd) of 2.2 x 10(-10)M, and 55,000 high-affinity PDGF-BB binding sites per cell, with a Kd of 1.2 x 10(-10)M. The functional consequence of PDGF binding was also assessed. PDGF-AA and PDGF-BB both stimulated migration of normal human osteoblastic cells and stimulated thymidine incorporation. To gain insight into potential transmodulation of the PDGF response, we investigated the capacity of interleukin-1 beta (IL-1 beta), a cytokine that induces bone resorption, to modulate PDGF binding and PDGF-induced biological activity. IL-1 beta significantly reduced PDGF-AA binding and significantly decreased both PDGF-AA-mediated cell migration and thymidine incorporation. In contrast, IL-1 beta had only a small effect of PDGF-BB binding and PDGF-BB-induced biological activity in normal human osteoblastic cells.

The Biological Activity in Vivo of Recombinant Murine Interleukin 1 in the Rat

1986 ◽  
Vol 182 (2) ◽  
pp. 263-271 ◽  
Author(s):  
R. Tocco-Bradley ◽  
L. L. Moldawer ◽  
C. T. Jones ◽  
B. Gerson ◽  
G. L. Blackburn ◽  
...  
Keyword(s):  
Biological Activity ◽  
Interleukin 1

scholarly journals Binding and internalization of interleukin 1 by T cells. Direct evidence for high- and low-affinity classes of interleukin 1 receptor.

1986 ◽  
Vol 164 (4) ◽  
pp. 1060-1074 ◽  
Author(s):  
J W Lowenthal ◽  
H R MacDonald
Keyword(s):  
T Cells ◽  
Biological Activity ◽  
Direct Evidence ◽  
Interleukin 1 ◽  
Cell Types ◽  
Absolute Number ◽  
Binding Assays ◽  
High Affinity ◽  
Large Numbers ◽  
A Minor

In this report we describe, on the basis of direct IL-1 binding assays and IL-1 internalization studies, the existence of two classes of IL-1-R on a variety of T cell types. Cells of the EL4-6.1 thymoma express large numbers (approximately 20,000 per cell) of IL-1-R that have a Kd of approximately 300 pM for IL-1. Even though these receptors make up 98-99% of the total IL-1-R per cell, they appear to be nonfunctional, based on their inability to endocytose IL-1. A minor class of IL-1-R (200-400/cell) has an approximately 100-fold higher affinity for IL-1 (Kd, approximately 5 pM) and can rapidly internalize the ligand upon binding. All of the biological activity of IL-1 can be shown to occur via binding to high-affinity IL-1-R since the IL-1 concentration giving half-maximum biological activity in EL4-6.1 cells corresponds precisely to the Kd of this class of receptor. Other cell types, including normal T cells, also express both high- and low-affinity IL-1-R, but the absolute number of receptors per cell is considerably less.

scholarly journals A point mutation uncouples human interleukin-1 beta biological activity and receptor binding.

1990 ◽  
Vol 265 (11) ◽  
pp. 5922-5925
Author(s):  
L Gehrke ◽  
S A Jobling ◽  
L S Paik ◽  
B McDonald ◽  
L J Rosenwasser ◽  
...  
Keyword(s):  
Biological Activity ◽  
Point Mutation ◽  
Receptor Binding ◽  
Interleukin 1 ◽  
Interleukin 1 Beta

The purification and biological activity of a macrophage-derived factor with interleukin 1-like activities from guinea pigs

1986 ◽  
Vol 881 (3) ◽  
pp. 437-445 ◽  
Author(s):  
Kaoru Onoue ◽  
Takumi Sasaki ◽  
Yusei Yamamoto ◽  
Bi-Hu Lin ◽  
Hiromi Matsuda
Keyword(s):  
Biological Activity ◽  
Guinea Pigs ◽  
Interleukin 1
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