Alkaline Phosphatase in Red Cell Membrane: Interconnection of Activities and Membrane Lipid Fluidity

1980 ◽  
pp. 131-139 ◽  
Author(s):  
Christina Ziemann ◽  
Guido Zimmer
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Membrane ◽  
Membrane Lipid ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Lipid Fluidity ◽  
Membrane Lipid Fluidity

scholarly journals Biosynthetic insulin effect on red cell membrane lipid peroxidation in patients with insulin-dependent diabetes

1994 ◽  
Vol 40 (3) ◽  
pp. 12-15 ◽  
Author(s):  
T. S. Balashova ◽  
Ye. N. Golega ◽  
I. A. Rudko ◽  
M. I. Balabolkin ◽  
A. A. Kubatiev
Keyword(s):  
Cell Membrane ◽  
Human Insulin ◽  
Antioxidant Defense ◽  
Vascular Complications ◽  
Membrane Lipid ◽  
Defense Enzymes ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Red Cell Membrane ◽  
Antioxidant Defense Enzymes

The aim of our research was elucidation of a relationship between red cell membrane lipid peroxidation (LPO) and antioxidant' defense enzymes, on the one hand and the age disease duration and presence of vascular complications in patients with type I diabetes mellitus on the other. The possibility of correcting red cell peroxide status with human insulin preparations was investigated. Red cell membrane LPO was found increased more than twofold and antioxidant defense enzymes activities virtually unchanged vs. controls in 16 patients with diabetes aged 20 to 43. These characteristics of red cell peroxidation status do not depend on patients age disease standing or presence of vascular complications. A twelve-week therapy with biosynthetic insulin resulted in complete normalization of LPO processes in patients with angiopathies aged under 35 and with disease standing of less than 10 years. In diabetics with angiopathies aged over 35 and disease standing of more than 10 years red cell MDA level reduced under the effect of therapy with human insulin preparations but was still increased vs. that in healthy donors by 1.5 times. Red cell GP and SOD activities reduced in the course of insulin therapy in all the examined groups of diabetics. Catalase activity increased by approximately 50% in patients with angiopathies those aged over 35 and a disease standing of more than 10 years under the effect of insulin. In the rest groups of patients catalase activity did not differ from its initial level. Our results permit us recommending besides human insulin preparations antioxidant therapy for patients with vascular complications those aged over 35 and a disease standing of more than 10 years.

Disorders of the red cell membrane

2020 ◽  
pp. 5456-5463
Author(s):  
Patrick G. Gallagher
Keyword(s):  
Cell Membrane ◽  
Clinical Features ◽  
Parvovirus B19 ◽  
Clinical Manifestations ◽  
Peripheral Blood Smear ◽  
Membrane Lipid ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Membrane Lipid Bilayer ◽  
Parvovirus B19 Infection

The integrity of the red cell membrane depends on molecular interactions between proteins and the phospholipid membrane: vertical interactions stabilize the membrane lipid bilayer; horizontal interactions provide resistance against shear stress. Hereditary spherocytosis—affects 1 in 25 000 individuals of northern European descent. There is typically a dominant family history, but the condition is genetically heterogeneous: combined spectrin and ankyrin deficiency is the most common defect observed, followed by band 3 deficiency, isolated spectrin deficiency, and protein 4.2 deficiency. These affect vertical membrane interactions with loss of surface area relative to red cell volume. Clinical features—the key clinical manifestations are anaemia and signs of persistent haemolysis, with jaundice and a marked propensity to gallstones. Complications and treatment—parvovirus B19 infection of erythropoietic precursors may cause acute aplastic crises. Megaloblastic anaemia due to folate deficiency occurs in response to increased requirements during growth and pregnancy, but is preventable with supplementation. Splenectomy can alleviate the anaemia in many patients and reduces the risk of gallstones. Hereditary elliptocytosis—occurs with a frequency of 1 in 2000 to 1 in 4000 worldwide, and is more frequent in parts of Africa. The inheritance is usually dominant, with defects in red cell proteins such as α‎- and β‎-spectrin causing disturbances in horizontal interactions in the erythrocyte membrane. Clinical features, diagnosis, and treatment—most patients are asymptomatic and are typically diagnosed incidentally during testing for unrelated conditions, but about 10% experience haemolysis, anaemia, splenomegaly, and intermittent jaundice. Diagnosis is based on the presence of elliptocytes on a peripheral blood smear. Treatment is rarely required. Other conditions include hereditary pyropoikilocytosis, South-East Asian (or Melanesian) ovalocytosis, stomatocytosis, and acanthocytosis.

scholarly journals Decreased life span and membrane damage of carbamylated erythrocytes in vitro

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 909-917 ◽  
Author(s):  
TA Lane ◽  
ER Burka
Keyword(s):  
Cell Membrane ◽  
Life Span ◽  
Membrane Damage ◽  
Membrane Lipid ◽  
Red Cell ◽  
Significant Modification ◽  
Red Cell Membrane ◽  
Cell Membrane Damage ◽  
Sickle Erythrocytes

Abstract Red blood cells exposed to cyanate (CNO) in vitro have a concentration- dependent decreased cell survival time associated with an inhibition of the ability of the cell membrane to synthesize lipids. The t1/2 of rabbit erythrocytes exposed to 30 mM or 50 mM cyanate for 1 hr at 37 degrees C is reduced from the normal 24 days to 15 and 9 days, respectively. The cyanate-induced defect in membrane lipid metabolism is irreversible. Carbamylation of membrane proteins and damage to metabolism are minimized by limiting exposure in vitro to 15 mM cyanate at 4 degrees C for 30 min. Cells carbamylated under these conditions do not have a shortened life span. Levels of globin carbamylation of 0.5 moles CNO/mole hemoglobin, shown to be clinically effective in prolonging the life span of sickle erythrocytes, are obtained under these conditions and reach maximal levels after only 30 min of incubation. Carbamylation of blood in CPD anticoagulant is inferior to either ACD or heparin. The findings indicate that adequate carbamylation of sickle erythrocytes with minimal red cell membrane damage can be achieved without significant modification of the standard plasmapheresis procedure utilized by the working blood bank.

Red Cell Membrane Lipid Peroxidation and Hemolysis Secondary to Phototherapy

1985 ◽  
Vol 74 (3) ◽  
pp. 378-381 ◽  
Author(s):  
ENRIQUE M. OSTREA ◽  
EUGENE E. CEPEDA ◽  
CHERYL A. FLEURY ◽  
JAMES E. BALUN
Keyword(s):  
Lipid Peroxidation ◽  
Cell Membrane ◽  
Membrane Lipid ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Red Cell Membrane

Red Cell Membrane Lipid Changes at 3500 m and on Return to Sea Level

2005 ◽  
Vol 6 (4) ◽  
pp. 320-326 ◽  
Author(s):  
Gustavo González ◽  
Gloria Celedón ◽  
Marcela Escobar ◽  
Carlos Sotomayor ◽  
Verónica Ferrer ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Sea Level ◽  
Membrane Lipid ◽  
Red Cell ◽  
Red Cell Membrane

scholarly journals Decreased life span and membrane damage of carbamylated erythrocytes in vitro

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 909-917
Author(s):  
TA Lane ◽  
ER Burka
Keyword(s):  
Cell Membrane ◽  
Life Span ◽  
Membrane Damage ◽  
Membrane Lipid ◽  
Red Cell ◽  
Significant Modification ◽  
Red Cell Membrane ◽  
Cell Membrane Damage ◽  
Sickle Erythrocytes

Red blood cells exposed to cyanate (CNO) in vitro have a concentration- dependent decreased cell survival time associated with an inhibition of the ability of the cell membrane to synthesize lipids. The t1/2 of rabbit erythrocytes exposed to 30 mM or 50 mM cyanate for 1 hr at 37 degrees C is reduced from the normal 24 days to 15 and 9 days, respectively. The cyanate-induced defect in membrane lipid metabolism is irreversible. Carbamylation of membrane proteins and damage to metabolism are minimized by limiting exposure in vitro to 15 mM cyanate at 4 degrees C for 30 min. Cells carbamylated under these conditions do not have a shortened life span. Levels of globin carbamylation of 0.5 moles CNO/mole hemoglobin, shown to be clinically effective in prolonging the life span of sickle erythrocytes, are obtained under these conditions and reach maximal levels after only 30 min of incubation. Carbamylation of blood in CPD anticoagulant is inferior to either ACD or heparin. The findings indicate that adequate carbamylation of sickle erythrocytes with minimal red cell membrane damage can be achieved without significant modification of the standard plasmapheresis procedure utilized by the working blood bank.

Role of red cell membrane lipid peroxidation in hemolysis due to phenylhydrazine

1980 ◽  
Vol 29 (10) ◽  
pp. 1355-1359 ◽  
Author(s):  
Bernard D. Goldstein ◽  
Michael G. Rozen ◽  
Richard L. Kunis
Keyword(s):  
Lipid Peroxidation ◽  
Cell Membrane ◽  
Membrane Lipid ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Red Cell Membrane

Red cell membrane lipid peroxidation in transfusion-dependent B and EB thalassemia

1990 ◽  
Vol 43 (2) ◽  
pp. 101-104 ◽  
Author(s):  
P. Lahiri ◽  
S. Bhattacharjee ◽  
S. Chandra ◽  
D.K. Bhattacharya
Keyword(s):  
Lipid Peroxidation ◽  
Cell Membrane ◽  
Membrane Lipid ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Red Cell Membrane
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