The three-dimensional ultrastructure of intracellular organization of smooth muscle cells by scanning electron microscopy

1990 ◽  
pp. 63-77 ◽  
Author(s):  
Takao Inoué
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Intracellular Organization ◽  
Scanning Electron

Preparation of recombinant human-like collagen/fibroin scaffold and its promoting effect on vascular cells biocompatibility

2018 ◽  
Vol 33 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Jia Yan ◽  
Kun Hu ◽  
YongHao Xiao ◽  
Fan Zhang ◽  
Lu Han ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Laser Scanning ◽  
Muscle Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

A novel recombinant human-like collagen/fibroin scaffold has been prepared previously, which has high porosity, controllable pore size, and much better mechanical properties than the reported fibroin-based scaffold. In this research, the cell responses of vascular smooth muscle cells to this blend scaffold were examined in vitro. Cell morphology, adherence, and growth in scaffolds were observed by scanning electron microscopy, laser scanning confocal microscopy after staining of the cells with propidium iodide at 1, 3, 5, and 7 days, respectively. A wide range of measurements, including 3-[4,5–dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide assay, and total intracellular protein content at the end of 7 days culture, were conducted. An increase of viability and protein content of vascular smooth muscle cells cultured in recombinant human-like collagen/fibroin scaffold was found. The laser scanning confocal microscopy and scanning electron microscopy results confirm that the cells readily adhered and proliferation in the blend than in fibroin scaffold, and indicate a better adhesion process. The positive effects were especially significant for vascular smooth muscle cells. The recombinant human-like collagen/fibroin scaffold could be a promising biomaterial for vascular tissue engineering.

Digestion techniques for exposure of smooth muscle cells in cerebral arterioles

1989 ◽  
Vol 47 ◽  
pp. 942-943
Author(s):  
S. Ghoneim ◽  
G. Baumbach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smooth Muscle ◽  
Basement Membrane ◽  
Three Dimensional ◽  
Type Ii ◽  
Sprague Dawley ◽  
Packed Red Blood Cells ◽  
Cerebral Arterioles ◽  
Scanning Electron

Scanning electron microscopy has proven to be a useful tool for the quantitation of three- dimensional characteristics of vascular smooth muscle. A major impediment to obtaining useful preparations of cerebral blood vessels for scanning electron microscopy is that smooth muscle in the tunica media is covered by a thin layer of basement membrane and by arachnoid tissue which contains collagen. The goal of this study was to develop a reliable, reproducible method for removing basement membrane and arachnoid tissue from cerebral arterioles, while at the same time leaving the smooth muscle intact.We compared several methods of tissue digestion in pial arterioles in adult Sprague-Dawley rats. The cerebral circulation was perfused with glutaraldehyde (2.5%) in cacodylate buffer (0.1 M) via the ascending aorta and then reinfused with packed red blood cells. In one group of rats, whole brains were immersed in osmium tetroxide (2%) for 2 hours (1 hr at 5°C and 1 hr at 40°C), followed by immersion in HC1 (8 N at 60°C) for 20-25 minutes. In another group, whole brains were dipped briefly in HC1 (8 N at 60°C) and then immersed in collagenase type II (2 mg/ml at 37° C; Sigma) for 12 hours. In a third group of rats, individual arterioles were dissected from the brain with a microsurgical knife, dipped briefly in HC1 (5-10 seconds) or KOH (5M at 60°C for 2-3minutes), and immersed in collagenase type II alone (12 hours), or in combination with collagenase type IV (2 mg/ml; 6-12 hours) and/or pepsin (2 mg/ml; 6-12 hours).

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

1968 ◽  
Vol 26 ◽  
pp. 164-165
Author(s):  
R. I. Johnsson-Hegyeli ◽  
A. F. Hegyeli ◽  
D. K. Landstrom ◽  
W. C. Lane
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Cultures ◽  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Three Dimensional ◽  
Malignant Cell ◽  
Interference Microscopy ◽  
Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

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