In vitro sensitivity of human endometrial cancer to cytotoxic and biologic agents

1989 ◽  
pp. 117-126
Author(s):  
Charles E. Welander ◽  
Charles M. Jones ◽  
David S. Alberts ◽  
Sydney E. Salmon
Keyword(s):  
Endometrial Cancer ◽  
Biologic Agents ◽  
In Vitro Sensitivity

In vitro sensitivity of human endometrial cancer cell lines to paclitaxel or irinotecan (CPT-11) in combination with other aniticancer drugs

2000 ◽  
Vol 11 (7) ◽  
pp. 573-578 ◽  
Author(s):  
Hisakazu Paul Hiramatsu ◽  
Yoshihiro Kikuchi ◽  
Hiroshi Seto ◽  
Ichiro Nagata
Keyword(s):  
Endometrial Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Endometrial Cancer Cell ◽  
In Vitro Sensitivity

Ginkgolic acid induces apoptosis and autophagy of endometrial carcinoma cells via inhibiting PI3K/Akt/mTOR pathway in vivo and in vitro

2021 ◽  
pp. 096032712110237
Author(s):  
L Zhou ◽  
S Li ◽  
J Sun
Keyword(s):  
Endometrial Cancer ◽  
B Cells ◽  
Western Blot ◽  
Signal Pathway ◽  
Mtor Pathway ◽  
Related Protein ◽  
Western Blot Assay ◽  
Ginkgolic Acid

Endometrial cancer (EC) is the fourth most common malignancy in women in developed countries. The prognosis of EC is extremely poor, and it is an important factor that contributes to the death of patients. Therefore, studying EC pathogenesis and therapeutic targets, and exploring effective drugs are the primary tasks to improve the prognosis of EC. In the present study, we aimed to explore the function of ginkgolic acid (GA) in EC cell apoptosis and autophagy through PI3K/Akt/mTOR signal pathway in vitro and in vivo. Firstly, MTT assay and clone formation assay were employed to analyze the Ishikawa and HEC-1-B cell viabilities and proliferation after treatment with GA. The results showed that GA inhibited endometrial cancer cell survival. Flow cytometry assay and western blot assay were applied to examine the apoptosis and apoptosis related protein Bcl-2, Bax, Cleaved caspase-3 expression levels of Ishikawa and HEC-1-B cells after treatment with GA. Next, we applied western blot assay to analyze the autophagy associated proteins LC3I, LC3II, p62 and Beclin-1 in GA treated Ishikawa and HEC-1-B cells. We found that GA promoted apoptosis and induced autophagy of endometrial cancer cells. Meanwhile, western blot assay was also used to determine the expression levels of the PI3K/Akt/mTOR signal pathway related protein and the results revealed that GA inhibited the activity of PI3K/Akt/mTOR pathway. Finally, we found that GA inhibited tumor growth in vivo through immunohistochemistry assay. In conclusion, GA induces apoptosis and autophagy of EC cells via inhibiting PI3K/Akt/mTOR pathway in vivo and vitro.

scholarly journals Inactivating Mutations of the IK Gene Weaken Ku80/Ku70-Mediated DNA Repair and Sensitize Endometrial Cancer to Chemotherapy

Cancers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2487
Author(s):  
Chao Gao ◽  
Guangxu Jin ◽  
Elizabeth Forbes ◽  
Lingegowda S. Mangala ◽  
Yingmei Wang ◽  
...  
Keyword(s):  
Dna Repair ◽  
Endometrial Cancer ◽  
Protein Interactions ◽  
Somatic Mutations ◽  
The Cancer Genome Atlas ◽  
Response To Chemotherapy ◽  
Tcga Dataset ◽  
Inactivating Mutations

IK is a mitotic factor that promotes cell cycle progression. Our previous investigation of 271 endometrial cancer (EC) samples from the Cancer Genome Atlas (TCGA) dataset showed IK somatic mutations were enriched in a cluster of patients with high-grade and high-stage cancers, and this group had longer survival. This study provides insight into how IK somatic mutations contribute to EC pathophysiology. We analyzed the somatic mutational landscape of IK gene in 547 EC patients using expanded TCGA dataset. Co-immunoprecipitation and mass spectrometry were used to identify protein interactions. In vitro and in vivo experiments were used to evaluate IK’s role in EC. The patients with IK-inactivating mutations had longer survival during 10-year follow-up. Frameshift and stop-gain were common mutations and were associated with decreased IK expression. IK knockdown led to enrichment of G2/M phase cells, inactivation of DNA repair signaling mediated by heterodimerization of Ku80 and Ku70, and sensitization of EC cells to cisplatin treatment. IK/Ku80 mutations were accompanied by higher mutation rates and associated with significantly better overall survival. Inactivating mutations of IK gene and loss of IK protein expression were associated with weakened Ku80/Ku70-mediated DNA repair, increased mutation burden, and better response to chemotherapy in patients with EC.

scholarly journals In Vitro Sensitivity to Antibiotics of Swedish Strains of Mycoplasma Gallisepticum

1965 ◽  
Vol 6 (1) ◽  
pp. 234-238
Author(s):  
Lena Silvén
Keyword(s):  
Mycoplasma Gallisepticum ◽  
In Vitro Sensitivity

Progesterone induces apoptosis by activation of caspase-8 and calcitriol via activation of caspase-9 pathways in ovarian and endometrial cancer cells in vitro

APOPTOSIS ◽  
2021 ◽  
Author(s):  
Latoya McGlorthan ◽  
Ana Paucarmayta ◽  
Yovanni Casablanca ◽  
G. Larry Maxwell ◽  
Viqar Syed
Keyword(s):  
Endometrial Cancer ◽  
Cancer Cells ◽  
Caspase 8 ◽  
Caspase 9

In Vitro Sensitivity Analysis of the Gastrointestinal Dissolution Profile of Weakly Basic Drugs in the Stomach-to-Intestine Fluid Changing System: Explanation for Variable Plasma Exposure after Oral Administration

2021 ◽  
Vol 18 (4) ◽  
pp. 1711-1719
Author(s):  
Toshihide Takagi ◽  
Takato Masada ◽  
Keiko Minami ◽  
Makoto Kataoka ◽  
Ken-ichi Izutsu ◽  
...  
Keyword(s):  
Sensitivity Analysis ◽  
Oral Administration ◽  
Dissolution Profile ◽  
Basic Drugs ◽  
Plasma Exposure ◽  
In Vitro Sensitivity

scholarly journals Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b

2017 ◽  
Vol 38 (10) ◽  
pp. 1394-1400 ◽  
Author(s):  
Ya-ling Wang ◽  
Yuan Shen ◽  
Jian-ping Xu ◽  
Kun Han ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Cancer Cells

scholarly journals Four years’ monitoring of in vitro sensitivity and candidate molecular markers of resistance of Plasmodium falciparum to artesunate-mefloquine combination in the Thai-Myanmar border

2014 ◽  
Vol 13 (1) ◽  
pp. 23 ◽  
Author(s):  
Papichaya Phompradit ◽  
Poonuch Muhamad ◽  
Raewadee Wisedpanichkij ◽  
Wanna Chaijaroenkul ◽  
Kesara Na-Bangchang
Keyword(s):  
Plasmodium Falciparum ◽  
Molecular Markers ◽  
In Vitro Sensitivity

In Vitro Sensitivity of Probiotics to Human Pancreatic Juice

2008 ◽  
Vol 42 ◽  
pp. S170-S173 ◽  
Author(s):  
Mario Del Piano ◽  
Paolo Strozzi ◽  
Michela Barba ◽  
Serena Allesina ◽  
Francesca Deidda ◽  
...  
Keyword(s):  
Pancreatic Juice ◽  
In Vitro Sensitivity
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