Regulation of Mammary Epithelial Cell Proliferation: An In Vitro Mouse Mammary Epithelial Cell Model System

1986 ◽  
pp. 31-41 ◽  
Author(s):  
W. Imagawa ◽  
G. Bandyopadhyay ◽  
M. Spencer ◽  
J. Li ◽  
S. Nandi
Keyword(s):  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cell Model ◽  
Model System ◽  
Mammary Epithelial ◽  
Epithelial Cell Proliferation ◽  
Mouse Mammary Epithelial Cell ◽  
Cell Model System ◽  
Mammary Epithelial Cell Proliferation

scholarly journals Pleiotropic effects of FGFR1 on cell proliferation, survival, and migration in a 3D mammary epithelial cell model

2005 ◽  
Vol 171 (4) ◽  
pp. 663-673 ◽  
Author(s):  
Wa Xian ◽  
Kathryn L. Schwertfeger ◽  
Tracy Vargo-Gogola ◽  
Jeffrey M. Rosen
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Molecular Mechanisms ◽  
Cell Model ◽  
Smooth Muscle Actin ◽  
Three Dimensional ◽  
Mammary Epithelial ◽  
Matrix Metalloproteinase 3

Members of the fibroblast growth factor (FGF) family and the FGF receptors (FGFRs) have been implicated in mediating various aspects of mammary gland development and transformation. To elucidate the molecular mechanisms of FGFR1 action in a context that mimics polarized epithelial cells, we have developed an in vitro three-dimensional HC11 mouse mammary epithelial cell culture model expressing a drug-inducible FGFR1 (iFGFR1). Using this conditional model, iFGFR1 activation in these growth-arrested and polarized mammary acini initially led to reinitiation of cell proliferation, increased survival of luminal cells, and loss of cell polarity, resulting in the disruption of acinar structures characterized by the absence of an empty lumen. iFGFR1 activation also resulted in a gain of invasive properties and the induction of matrix metalloproteinase 3 (MMP-3), causing the cleavage of E-cadherin and increased expression of smooth muscle actin and vimentin. The addition of a pan MMP inhibitor abolished these phenotypes but did not prevent the effects of iFGFR1 on cell proliferation or survival.

Sign in / Sign up

Export Citation Format

Share Document