Lung Cells Grown on Cellulose Membrane Filters as an In Vitro Model of the Respiratory Epithelium

1982 ◽  
pp. 105-120 ◽  
Author(s):  
Ronald E. Rasmussen ◽  
T. Timothy Crocker
Keyword(s):  
In Vitro Model ◽  
Respiratory Epithelium ◽  
Lung Cells ◽  
Cellulose Membrane ◽  
Membrane Filters ◽  
Vitro Model

scholarly journals Assessment ofPasteurella multocidaA Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Carolina Gallego ◽  
Stefany Romero ◽  
Paula Esquinas ◽  
Pilar Patiño ◽  
Nhora Martínez ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
In Vitro Model ◽  
Cytoplasmic Membrane ◽  
Goblet Cells ◽  
Respiratory Epithelium ◽  
Host Cells ◽  
Transmission Electron ◽  
Vitro Model

The role of theP. multocidalipopolysaccharide (LPS) as a putative adhesin during the early stages of infection with this bacterium in the respiratory epithelium of rabbits was investigated. By light microscopy and double enzyme labeling of nasal septa tissues, the amount of bacteria attached to the respiratory epithelium and the amount of LPS present in goblet cells at different experimental times were estimated. Transmission electron microscopy (TEM) and LPS labeling with colloidal gold particles were also used to determine the exact location of LPS in the cells. Septa that were challenged with LPS ofP. multocidaand 30 minutes later withP. multocidashowed more adherent bacteria and more severe lesions than the other treatments. Free LPS was observed in the lumen of the nasal septum, forming bilamellar structures and adhering to the cilia, microvilli, cytoplasmic membrane, and cytoplasm of epithelial ciliated and goblet cells. The above findings suggest thatP. multocidaLPS plays an important role in the process of bacterial adhesion and that it has the ability of being internalized into host cells.

Role of carbohydrates in repair of human respiratory epithelium using an in vitro model

2003 ◽  
Vol 33 (10) ◽  
pp. 1398-1404 ◽  
Author(s):  
E. C. Adam ◽  
S. T. Holgate ◽  
C. J. Fildew ◽  
P. M. Lackie
Keyword(s):  
In Vitro Model ◽  
Respiratory Epithelium ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Präsidentenposter: Regulation der Expression der β-Defensine hBD-2 und hBD-3 in einem in vitro Model der Candida Ösophagitis: NF-κB, MAPK/AP-1 und EGFR

2009 ◽  
Vol 47 (09) ◽  
Author(s):  
N Steubesand ◽  
K Kiehne ◽  
R Pahl ◽  
G Brunke ◽  
UR Fölsch ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Vitro Model

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model
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