The High-Affinity Folate Binding Protein in Normal and Malignant Mammary Gland Tissue

1993 ◽  
pp. 757-760 ◽  
Author(s):  
Jan Holm ◽  
Steen I. Hansen ◽  
Knud Søndergaard ◽  
Mimi Høier-Madsen
Keyword(s):  
Mammary Gland ◽  
Binding Protein ◽  
Folate Binding Protein ◽  
High Affinity ◽  
Mammary Gland Tissue ◽  
Gland Tissue

High-affinity folate binding in human mammary gland

1993 ◽  
Vol 13 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
Mimi Høier-Madsen
Keyword(s):  
Mammary Gland ◽  
Binding Protein ◽  
Cross Reactivity ◽  
Binding Activity ◽  
Gel Chromatography ◽  
Folate Binding Protein ◽  
High Affinity ◽  
Mammary Gland Tissue ◽  
Human Mammary Gland ◽  
Triton X

High-affinity 3H-folate binding in Triton X-100 solubilized human mammary gland tissue displayed characteristics, e.g. apparent positive cooperativity and increasing affinity with decreasing concentration of folate binding protein, shown to be typical of specific folate binding. Radioligand dissociation was slow at pH 7.4. A major fraction of the bound radioligand dissociated rapidly at pH 3.5, while a residual binding of 20% persisted even after prolonged dialysis at pH 3.5. Gel chromatography revealed two major folate binding proteins (Mr ≈ 100 kDa and 25 kDa). However, only one single band was detectable on SDS-PAGE immunoblotting. The highest folate binding activity per g protein was associated with the upper triglyceride-containing layer of the 1000 g supernatant of the homogenate. The folate binding protein extracted from this layer had a low cross-reactivity (<5%) with rabbit antibodies against 25 kDa human milk folate binding protein. The folate binding protein in the 1000 g pellet and the aqueous phase of the 1000 g supernatant was present at a low concentration and had a cross-reactivity of 100%.

PROTEOME OF MILK FAT GLOBULE MEMBRANE AND MAMMARY GLAND TISSUE IN GOAT FED DIFFERENT LIPID SUPPLEMENTATION

2021 ◽  
pp. 106378
Author(s):  
Iolly Tábata Oliveira Marques ◽  
Fábio Roger Vasconcelos ◽  
Juliana Paula Martins Alves ◽  
Assis Rubens Montenegro ◽  
César Carneiro Linhares Fernandes ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Milk Fat ◽  
Milk Fat Globule Membrane ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Milk Fat Globule ◽  
Lipid Supplementation ◽  
Fat Globule

A high affinity folate binding protein in umbilical cord serum

1980 ◽  
Vol 40 (6) ◽  
pp. 523-527 ◽  
Author(s):  
Jan Holm ◽  
Steen Ingemann Hansen ◽  
Jörgen Lyngbye
Keyword(s):  
Umbilical Cord ◽  
Binding Protein ◽  
Folate Binding Protein ◽  
Cord Serum ◽  
High Affinity

Spectral Characterization of Fluorescently Labeled Catechol Estrogen 3,4-Quinone-Derived N7 Guanine Adducts and Their Identification in Rat Mammary Gland Tissue

1998 ◽  
Vol 11 (11) ◽  
pp. 1339-1345 ◽  
Author(s):  
Ryszard Jankowiak ◽  
Dan Zamzow ◽  
Douglas E. Stack ◽  
Rosa Todorovic ◽  
Ercole L. Cavalieri ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Spectral Characterization ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Guanine Adducts ◽  
Catechol Estrogen ◽  
Rat Mammary Gland

scholarly journals Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Johanna Brodhagen ◽  
Rosemarie Weikard ◽  
Ulrike Thom ◽  
Annika Heimes ◽  
Juliane Günther ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Transcriptome Analysis ◽  
Milk Protein ◽  
Mammary Gland Tissue ◽  
Gland Tissue
Sign in / Sign up

Export Citation Format

Share Document