Pharmacogenetics Polymorphisms in Xenobiotic Metabolism

1999 ◽  
pp. 91-110 ◽  
Author(s):  
Frank J. Gonzalez
Keyword(s):  
Xenobiotic Metabolism

Influence of Slice Thickness and Culture Conditions on the Metabolism of 7-Ethoxycoumarin in Precision-cut Rat Liver Slices

1998 ◽  
Vol 26 (4) ◽  
pp. 541-548
Author(s):  
Roger J. Price ◽  
Anthony B. Renwick ◽  
Paula T. Barton ◽  
J. Brian Houston ◽  
Brian G. Lake
Keyword(s):  
Gas Phase ◽  
Rat Liver ◽  
Xenobiotic Metabolism ◽  
Culture Conditions ◽  
Slice Thickness ◽  
Dependent Manner ◽  
Sprague Dawley ◽  
Liver Slices ◽  
Sprague Dawley Rats ◽  
Rat Livers

This study investigated the effects of some experimental variables on the rate of xenobiotic metabolism in precision-cut rat liver slices. Liver slices of 123 ± 8μm (mean ± SEM of six slices), 165 ± 3μm, 238 ± 6μm and 515 ± 14μm thickness were prepared from male Sprague-Dawley rats, and incubated in RPMI 1640 medium in an atmosphere of 95% O2/5% CO2 by using a dynamic organ culture system. Liver slices of all thicknesses metabolised 10μM 7-ethoxycoumarin to total (free and conjugated) 7-hydroxycoumarin in a time-dependent manner. The rate of 7-ethoxycoumarin metabolism was greatest in 165μm thick slices and slowest in 515μm thick slices, being 2.74 ± 0.19pmol/minute/mg slice protein and 0.69 ± 0.07pmol/minute/mg slice protein, respectively. No marked effects on the rate of 7-ethoxycoumarin metabolism in liver slices were observed either by changing the medium to Earle's balanced salt solution (EBSS) or by changing the gas phase to 95% air/5% CO2. Moreover, the perfusion of rat livers with EBSS at 2–4°C, prior to preparation of tissue cores, did not enhance 7-ethoxycoumarin metabolism in rat liver slices. In this study, the optimal slice thickness was 175μm, with higher rates of 7-ethoxycoumarin metabolism being observed than with 250μm thick slices, which are often used for studies of xenobiotic metabolism. Variable results were obtained with slices of around 100–120μm thickness, which may be attributable to the ratio between intact hepatocytes and cells damaged by the slicing procedure in these very thin slices.

scholarly journals Glossary and tutorial of xenobiotic metabolism terms used during small molecule drug discovery and development (IUPAC Technical Report)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Paul Erhardt ◽  
Kenneth Bachmann ◽  
Donald Birkett ◽  
Michael Boberg ◽  
Nicholas Bodor ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Small Molecule ◽  
Chemical Structure ◽  
Early Stage ◽  
Xenobiotic Metabolism ◽  
Drug Discovery And Development ◽  
Small Molecule Drug ◽  
Technical Report ◽  
Wide Range ◽  
Draft Version

Abstract This project originated more than 15 years ago with the intent to produce a glossary of drug metabolism terms having definitions especially applicable for use by practicing medicinal chemists. A first-draft version underwent extensive beta-testing that, fortuitously, engaged international audiences in a wide range of disciplines involved in drug discovery and development. It became clear that the inclusion of information to enhance discussions among this mix of participants would be even more valuable. The present version retains a chemical structure theme while expanding tutorial comments that aim to bridge the various perspectives that may arise during interdisciplinary communications about a given term. This glossary is intended to be educational for early stage researchers, as well as useful for investigators at various levels who participate on today’s highly multidisciplinary, collaborative small molecule drug discovery teams.

Interrelationships between xenobiotic metabolism and lipid biosynthesis

1983 ◽  
Vol 32 (11) ◽  
pp. 1667-1672 ◽  
Author(s):  
John Caldwell ◽  
Mary Varwell Marsh
Keyword(s):  
Xenobiotic Metabolism ◽  
Lipid Biosynthesis

Effect of peroxisome proliferators and inducers of xenobiotic metabolism on marker enzyme activities in cultured rat liver slices

1994 ◽  
Vol 8 (4) ◽  
pp. 521-523 ◽  
Author(s):  
B.G. Lake ◽  
J.A. Beamand ◽  
M.E. Cunninghame ◽  
S. Davies ◽  
H. Mistry ◽  
...  
Keyword(s):  
Rat Liver ◽  
Enzyme Activities ◽  
Xenobiotic Metabolism ◽  
Peroxisome Proliferators ◽  
Marker Enzyme ◽  
Liver Slices

scholarly journals Significance of Xenobiotic Metabolism for Bioaccumulation Kinetics of Organic Chemicals in Gammarus pulex

2012 ◽  
Vol 46 (6) ◽  
pp. 3498-3508 ◽  
Author(s):  
Roman Ashauer ◽  
Anita Hintermeister ◽  
Isabel O’Connor ◽  
Maline Elumelu ◽  
Juliane Hollender ◽  
...  
Keyword(s):  
Xenobiotic Metabolism ◽  
Gammarus Pulex ◽  
Organic Chemicals ◽  
Kinetics Of

Sulforaphane Regulates NFE2L2/Nrf2-Dependent Xenobiotic Metabolism Phase II and Phase III Enzymes Differently in Human Colorectal Cancer and Untransformed Epithelial Colon Cells

2016 ◽  
Vol 68 (8) ◽  
pp. 1338-1348 ◽  
Author(s):  
Katarzyna Lubelska ◽  
Katarzyna Wiktorska ◽  
Lidia Mielczarek ◽  
Małgorzata Milczarek ◽  
Ilona Zbroińska-Bregisz ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Phase Ii ◽  
Xenobiotic Metabolism ◽  
Phase Iii ◽  
Human Colorectal Cancer ◽  
Colon Cells
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