Extracellular Signaling in an Oscillatory Yeast Culture

1998 ◽  
pp. 85-94 ◽  
Author(s):  
Marc Keulers ◽  
Hiroshi Kuriyama
Keyword(s):  
Yeast Culture ◽  
Extracellular Signaling

EFFECT OF SACCHAROMYCES CEREVISIAE LIVE YEAST CULTURE SUPPLEMENTATION ON TISSUE GLUTATHIONE VALUES IN RABBITS.

2019 ◽  
Vol 38 (2) ◽  
Author(s):  
Nurten GALIP ◽  
Nilay SEYIDOGLU ◽  
Zehra SERDAR ◽  
Nilgün Savaş ◽  
Merve Akyıldız
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Culture ◽  
Live Yeast

V-ATPase Sub-Classifies IDHwt Lowergrade Gliomas and Directs Extracellular Signaling Through Large Oncosomes and Homeobox Genes

2018 ◽  
Author(s):  
Irene Bertolini ◽  
Andrea Terrasi ◽  
Cristina Martelli ◽  
Gabriella Gaudioso ◽  
Andrea Di Cristofori ◽  
...  
Keyword(s):  
Homeobox Genes ◽  
Extracellular Signaling

scholarly journals Zinc Sulfate Stimulates Osteogenic Phenotypes in Periosteum-Derived Cells and Co-Cultures of Periosteum-Derived Cells and THP-1 Cells

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 410
Author(s):  
Jin-Ho Park ◽  
Su A Park ◽  
Young-Hoon Kang ◽  
So Myeong Hwa ◽  
Eun-Byeol Koh ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Zinc Sulfate ◽  
Nuclear Factor Kappa B ◽  
Osteoblastic Differentiation ◽  
Nuclear Factor ◽  
Mitogen Activated Protein Kinases ◽  
Extracellular Signaling ◽  
Receptor Activator ◽  
Mitogen Activated Protein ◽  
Osteoclastic Differentiation

Coupling between osteoblast-mediated bone formation and osteoclast-mediated bone resorption maintains both mechanical integrity and mineral homeostasis. Zinc is required for the formation, mineralization, growth, and maintenance of bones. We examined the effects of zinc sulfate on osteoblastic differentiation of human periosteum-derived cells (hPDCs) and osteoclastic differentiation of THP-1 cells. Zinc sulfate enhanced the osteoblastic differentiation of hPDCs; however, it did not affect the osteoclastic differentiation of THP-1 cells. The levels of extracellular signaling-related kinase (ERK) were strongly increased during osteoblastic differentiation in zinc sulfate-treated hPDCs, compared with other mitogen-activated protein kinases (MAPKs). Zinc sulfate also promoted osteogenesis in hPDCs and THP-1 cells co-cultured with the ratio of one osteoclast to one osteoblast, as indicated by alkaline phosphatase levels, mineralization, and cellular calcium contents. In addition, the receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio was decreased in the zinc sulfate-treated co-cultures. Our results suggest that zinc sulfate enhances osteogenesis directly by promoting osteoblastic differentiation and osteogenic activities in osteoblasts and indirectly by inhibiting osteoclastic bone resorption through a reduced RANKL/OPG ratio in co-cultured osteoblasts and osteoclasts.

scholarly journals The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Xin Ping Li ◽  
Lu Lu Zhou ◽  
Yan Hua Guo ◽  
Jian Wen Wang
Keyword(s):  
Atp Release ◽  
Specific Inhibitor ◽  
Extracellular Atp ◽  
Oxidase Inhibitor ◽  
Disulfonic Acid ◽  
Early Event ◽  
Fungal Metabolites ◽  
Extracellular Signaling ◽  
Hypocrellin A

Abstract Background Adenosine 5′-triphosphate (ATP) plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in diverse physiological processes of animals and plants. However, there are less reports concerning the signaling role of microbial extracellular ATP (eATP). Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from bambusicolous Shiraia fungi. The co-culture of Shiraia sp. S9 and a bacterium Pseudomonas fulva SB1 isolated from Shiraia fruiting bodies was established for enhanced hypocrellin A (HA) production. The signaling roles of eATP to mediate hypocrellin biosynthesis were investigated in the co-culture. Results The co-culture induced release of eATP at 378 nM to the medium around 4 h. The eATP release was interdependent on cytosolic Ca2+ concentration and reactive oxygen species (ROS) production, respectively. The eATP production could be suppressed by the Ca2+ chelator EGTA or abolished by the channel blocker La3+, ROS scavenger vitamin C and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). The bacterium-induced H2O2 production was strongly inhibited by reactive blue (RB), a specific inhibitor of membrane purinoceptors, but dependent on the induced Ca2+ influx in the co-culture. On the other hand, the application of exogenous ATP (exATP) at 10–300 µM to Shiraia cultures also promoted fungal conidiation and HA production, both of which were blocked effectively by the purinoceptor inhibitors pyridoxalphosphate-6-azophenyl-2′, 4′-disulfonic acid (PPADS) and RB, and ATP hydrolase apyrase. Both the induced expression of HA biosynthetic genes and HA accumulation were inhibited significantly under the blocking of the eATP or Ca2+ signaling, and the scavenge of ROS in the co-culture. Conclusions Our results indicate that eATP release is an early event during the intimate bacterial–fungal interaction and eATP plays a signaling role in the bacterial elicitation on fungal metabolites. Ca2+ and ROS are closely linked for activation of the induced ATP release and its signal transduction. This is the first report on eATP production in the fungal–bacterial co-culture and its involvement in the induced biosynthesis of fungal metabolites. Graphic abstract

scholarly journals Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark ofCuratella americanaL. (Dilleniaceae) againstCandidaSpecies

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Cleyton Eduardo Mendes de Toledo ◽  
Patrícia Regina Santos ◽  
João Carlos Palazzo de Mello ◽  
Benedito Prado Dias Filho ◽  
Celso Vataru Nakamura ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Virulence Factors ◽  
Crude Extract ◽  
Ethyl Acetate Fraction ◽  
Yeast Culture ◽  
Ethnomedicinal Plant ◽  
Fluconazole Resistant ◽  
Resistant Strains ◽  
Preliminary Study ◽  
Curatella Americana

The ethnomedicinal plantCuratella americanaL. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds fromC. americanawere evaluated against the standard yeast strainsCandida albicans,C. tropicalis, andC. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against theCandidaspecies including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.

scholarly journals Ras-mediated activation of the TORC2–PKB pathway is critical for chemotaxis

2010 ◽  
Vol 190 (2) ◽  
pp. 233-245 ◽  
Author(s):  
Huaqing Cai ◽  
Satarupa Das ◽  
Yoichiro Kamimura ◽  
Yu Long ◽  
Carole A. Parent ◽  
...  
Keyword(s):  
Cell Migration ◽  
Actin Polymerization ◽  
Time Course ◽  
Specific Inhibitor ◽  
Ras Proteins ◽  
Extracellular Signaling ◽  
Upstream Regulator ◽  
Pkb Phosphorylation ◽  
G Protein Coupled

In chemotactic cells, G protein–coupled receptors activate Ras proteins, but it is unclear how Ras-associated pathways link extracellular signaling to cell migration. We show that, in Dictyostelium discoideum, activated forms of RasC prolong the time course of TORC2 (target of rapamycin [Tor] complex 2)-mediated activation of a myristoylated protein kinase B (PKB; PKBR1) and the phosphorylation of PKB substrates, independently of phosphatidylinositol-(3,4,5)-trisphosphate. Paralleling these changes, the kinetics of chemoattractant-induced adenylyl cyclase activation and actin polymerization are extended, pseudopodial activity is increased and mislocalized, and chemotaxis is impaired. The effects of activated RasC are suppressed by deletion of the TORC2 subunit PiaA. In vitro RasCQ62L-dependent PKB phosphorylation can be rapidly initiated by the addition of a PiaA-associated immunocomplex to membranes of TORC2-deficient cells and blocked by TOR-specific inhibitor PP242. Furthermore, TORC2 binds specifically to the activated form of RasC. These results demonstrate that RasC is an upstream regulator of TORC2 and that the TORC2–PKB signaling mediates effects of activated Ras proteins on the cytoskeleton and cell migration.

Visible-light-initiated hydrogels preserving cartilage extracellular signaling for inducing chondrogenesis of mesenchymal stem cells

2015 ◽  
Vol 12 ◽  
pp. 30-41 ◽  
Author(s):  
Bogyu Choi ◽  
Soyon Kim ◽  
Brian Lin ◽  
Kevin Li ◽  
Olga Bezouglaia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Visible Light ◽  
Extracellular Signaling

scholarly journals Dynamic Interactions and the Evolutionary Genetics of Dental Patterning

1998 ◽  
Vol 9 (4) ◽  
pp. 369-398 ◽  
Author(s):  
K.M. Weiss ◽  
D.W. Stock ◽  
Z. Zhao
Keyword(s):  
Morphological Structure ◽  
Homeobox Gene ◽  
Evolutionary Genetics ◽  
Gene Families ◽  
Developmental Genetics ◽  
Dynamic Interactions ◽  
Extracellular Signaling ◽  
Combinatorial Codes ◽  
Tooth Type ◽  
Mammalian Teeth

The mammalian dentition is a segmental, or periodically arranged, organ system whose components are arrayed in specific number and in regionally differentiated locations along the linear axes of the jaws. This arrangement evolved from simpler dentitions comprised of many single-cusp teeth of relatively indeterminate number. The different types of mammalian teeth have subsequently evolved as largely independent units. The experimentally documented developmental autonomy of dental primordia shows that the basic dental pattern is established early in embryogenesis. An understanding of how genetic patterning processes may work must be consistent with the different modes of development, and partially independent evolution, of the upper and lower dentition in mammals. The periodic nature of the location, number, and morphological structure of teeth suggests that processes involving the quantitative interaction of diffusible signaling factors may be involved. Several extracellular signaling molecules and their interactions have been identified that may be responsible for locating teeth along the jaws and for the formation of the incisor field. Similarly, the wavelike expression of signaling factors within developing teeth suggests that dynamic interactions among those factors may be responsible for crown patterns. These factors seem to be similar among different tooth types, but the extent to which crown differences can be explained strictly in terms of variation in the parameters of interactions among the same genes, as opposed to tooth-type-specific combinatorial codes of gene expression, is not yet known. There is evidence that combinatorial expression of intracellular transcription factors, including homeobox gene families, may establish domains within the jaws in which different tooth types are able to develop. An evolutionary perspective can be important for our understanding of dental patterning and the designing of appropriate experimental approaches, but dental patterns also raise basic unresolved questions about the nature of the evolutionary assumptions made in developmental genetics.

scholarly journals Effect of Live Yeast Culture Supplementation on Fibrolytic and Saccharolytic Bacterial Populations in the Feces of Horses Fed a High-Fiber or High-Starch Diet

2017 ◽  
Vol 51 ◽  
pp. 41-45 ◽  
Author(s):  
Jo-Anne Mary Davina Murray ◽  
Sheena Brown ◽  
Peter O'Shaughnessy ◽  
Ana Monteiro ◽  
Helen Warren ◽  
...  
Keyword(s):  
Yeast Culture ◽  
Bacterial Populations ◽  
High Fiber ◽  
High Starch ◽  
Live Yeast ◽  
Starch Diet
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