Cell cycle regulation of S phase entry in Saccharomyces cerevisiae

1997 ◽  
pp. 143-156 ◽  
Author(s):  
Simonetta Piatti
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
S Phase ◽  
Cycle Regulation ◽  
Phase Entry

scholarly journals Posttranslational Phosphorylation and Ubiquitination of the Saccharomyces cerevisiae Poly(A) Polymerase at the S/G2 Stage of the Cell Cycle

2000 ◽  
Vol 20 (8) ◽  
pp. 2794-2802 ◽  
Author(s):  
Neptune Mizrahi ◽  
Claire Moore
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Sorting ◽  
S Phase ◽  
Phase Arrest ◽  
Phosphatase Treatment ◽  
M Phase ◽  
64 Kda Protein ◽  
Mitotic Phosphorylation ◽  
Cycle Regulation

ABSTRACT The poly(A) polymerase of the budding yeast Saccharomyces cerevisiae (Pap1) is a 64-kDa protein essential for the maturation of mRNA. We have found that a modified Pap1 of 90 kDa transiently appears in cells after release from α-factor-induced G1 arrest or from a hydroxyurea-induced S-phase arrest. While a small amount of modification occurs in hydroxyurea-arrested cells, fluorescence-activated cell sorting analysis and microscopic examination of bud formation indicate that the majority of modified enzyme is found at late S/G2 and disappears by the time cells have reached M phase. The reduction of the 90-kDa product upon phosphatase treatment indicates that the altered mobility is due to phosphorylation. A preparation containing primarily the phosphorylated Pap1 has no poly(A) addition activity, but this activity is restored by phosphatase treatment. A portion of Pap1 is also polyubiquitinated concurrent with phosphorylation. However, the bulk of the 64-kDa Pap1 is a stable protein with a half-life of 14 h. The timing, nature, and extent of Pap1 modification in comparison to the mitotic phosphorylation of mammalian poly(A) polymerase suggest an intriguing difference in the cell cycle regulation of this enzyme in yeast and mammalian systems.

scholarly journals WEE1 kinase inhibitor AZD1775 induces CDK1 kinase-dependent origin firing in unperturbed G1- and S-phase cells

2019 ◽  
Vol 116 (48) ◽  
pp. 23891-23893 ◽  
Author(s):  
Tatiana N. Moiseeva ◽  
Chenao Qian ◽  
Norie Sugitani ◽  
Hatice U. Osmanbeyoglu ◽  
Christopher J. Bakkenist
Keyword(s):  
Cell Cycle ◽  
Clinical Trials ◽  
Cell Cycle Regulation ◽  
Kinase Inhibitor ◽  
S Phase ◽  
Origin Firing ◽  
Replicative Helicase ◽  
Origin Licensing ◽  
Wee1 Kinase ◽  
Cycle Regulation

WEE1 kinase is a key regulator of the G2/M transition. The WEE1 kinase inhibitor AZD1775 (WEE1i) induces origin firing in replicating cells. We show that WEE1i induces CDK1-dependent RIF1 phosphorylation and CDK2- and CDC7-dependent activation of the replicative helicase. WEE1 suppresses CDK1 and CDK2 kinase activities to regulate the G1/S transition after the origin licensing is complete. We identify a role for WEE1 in cell cycle regulation and important effects of AZD1775, which is in clinical trials.

scholarly journals Involvement of calcineurin‐dependent degradation of Yap1p in Ca 2+ ‐induced G 2 cell‐cycle regulation in Saccharomyces cerevisiae

EMBO Reports ◽  
2006 ◽  
Vol 7 (5) ◽  
pp. 519-524 ◽  
Author(s):  
Hiroshi Yokoyama ◽  
Masaki Mizunuma ◽  
Michiyo Okamoto ◽  
Josuke Yamamoto ◽  
Dai Hirata ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Cycle Regulation

scholarly journals Involvement of S-adenosylmethionine in G1 cell-cycle regulation in Saccharomyces cerevisiae

2004 ◽  
Vol 101 (16) ◽  
pp. 6086-6091 ◽  
Author(s):  
M. Mizunuma ◽  
K. Miyamura ◽  
D. Hirata ◽  
H. Yokoyama ◽  
T. Miyakawa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Cycle Regulation

scholarly journals Flexibility vs. robustness in cell cycle regulation of timing of M-phase entry in Xenopus laevis embryo cell-free extract

2016 ◽  
Vol 60 (7-8-9) ◽  
pp. 305-314
Author(s):  
Mateusz Debowski ◽  
Mohammed El Dika ◽  
Jacek Malejczyk ◽  
Robert Zdanowski ◽  
Claude Prigent ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Xenopus Laevis ◽  
Cell Cycle Regulation ◽  
Embryo Cell ◽  
Cell Free Extract ◽  
Laevis Embryo ◽  
Xenopus Laevis Embryo ◽  
M Phase ◽  
Cycle Regulation ◽  
Phase Entry

scholarly journals Cell Cycle Regulation of the Saccharomyces cerevisiae Polo-Like Kinase Cdc5p

1998 ◽  
Vol 18 (12) ◽  
pp. 7360-7370 ◽  
Author(s):  
Liang Cheng ◽  
Linda Hunke ◽  
Christopher F. J. Hardy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Kinase Activity ◽  
Anaphase Promoting Complex ◽  
Mitotic Kinases ◽  
Polo Kinase ◽  
Evolutionarily Conserved ◽  
M Phase ◽  
Cycle Regulation

ABSTRACT Progression through and completion of mitosis require the actions of the evolutionarily conserved Polo kinase. We have determined that the levels of Cdc5p, a Saccharomyces cerevisiae member of the Polo family of mitotic kinases, are cell cycle regulated. Cdc5p accumulates in the nuclei of G2/M-phase cells, and its levels decline dramatically as cells progress through anaphase and begin telophase. We report that Cdc5p levels are sensitive to mutations in key components of the anaphase-promoting complex (APC). We have determined that Cdc5p-associated kinase activity is restricted to G2/M and that this activity is posttranslationally regulated. These results further link the actions of the APC to the completion of mitosis and suggest possible roles for Cdc5p during progression through and completion of mitosis.

scholarly journals G1 Transcription Factors Are Differentially Regulated in Saccharomyces cerevisiae by the Swi6-Binding Protein Stb1

2003 ◽  
Vol 23 (14) ◽  
pp. 5064-5077 ◽  
Author(s):  
Michael Costanzo ◽  
Oliver Schub ◽  
Brenda Andrews
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Transcription Factors ◽  
Transcriptional Activation ◽  
Chromatin Immunoprecipitation ◽  
Cell Cycle Regulation ◽  
Binding Protein ◽  
Microarray Studies ◽  
Cycle Regulation ◽  
Integral Feature

ABSTRACT Stage-specific transcriptional programs are an integral feature of cell cycle regulation. In the budding yeast Saccharomyces cerevisiae, over 120 genes are coordinately induced in late G1 phase by two heterodimeric transcription factors called SBF and MBF. Activation of SBF and MBF is an upstream initiator of key cell cycle events, including budding and DNA replication. SBF and MBF regulation is complex and genetically redundant, and the precise mechanism of G1 transcriptional activation is unclear. Assays using SBF- and MBF-specific reporter genes revealed that the STB1 gene specifically affected MBF-dependent transcription. STB1 encodes a known Swi6-binding protein, but an MBF-specific function had not been previously suspected. Consistent with a specific role in regulating MBF, a STB1 deletion strain requires SBF for viability and microarray studies show a decrease in MBF-regulated transcripts in a swi4Δ mutant following depletion of Stb1. Chromatin immunoprecipitation experiments confirm that Stb1 localizes to promoters of MBF-regulated genes. Our data indicate that, contrary to previous models, MBF and SBF have unique components and might be distinctly regulated.

scholarly journals APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway

2016 ◽  
Vol 36 (5) ◽  
Author(s):  
Ajeena Ramanujan ◽  
Swati Tiwari
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Alternative Pathway ◽  
Retinoblastoma Protein ◽  
S Phase ◽  
Anaphase Promoting Complex ◽  
Anti Cancer ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway ◽  
Cycle Regulation

The ubiquitin (Ub) ligase anaphase promoting complex/cyclosome (APC/C) and the tumour suppressor retinoblastoma protein (pRB) play key roles in cell cycle regulation. APC/C is a critical regulator of mitosis and G1-phase of the cell cycle whereas pRB keeps a check on proliferation by inhibiting transition to the S-phase. APC/C and pRB interact with each other via the co-activator of APC/C, FZR1, providing an alternative pathway of regulation of G1 to S transition by pRB using a post-translational mechanism. Both pRB and FZR1 have complex roles and are implicated not only in regulation of cell proliferation but also in differentiation, quiescence, apoptosis, maintenance of chromosomal integrity and metabolism. Both are also targeted by transforming viruses. We discuss recent advances in our understanding of the involvement of APC/C and pRB in cell cycle based decisions and how these insights will be useful for development of anti-cancer and anti-viral drugs.

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