Regulation of Expression of Xenopus Vitellogenin Genes

1988 ◽  
pp. 241-265
Author(s):  
Jamshed R. Tata
Keyword(s):  
Regulation Of Expression ◽  
Vitellogenin Genes

scholarly journals Transcriptional regulation of expression of the rainbow trout albumin gene by estrogen

1998 ◽  
Vol 20 (3) ◽  
pp. 355-362 ◽  
Author(s):  
G Flouriot ◽  
B Ducouret ◽  
L Byrnes ◽  
Y Valotaire
Keyword(s):  
Rainbow Trout ◽  
Mrna Level ◽  
Primary Cultures ◽  
Transcriptional Level ◽  
Down Regulation ◽  
Dramatic Decrease ◽  
Regulation Of Expression ◽  
Albumin Gene ◽  
Run Off ◽  
Vitellogenin Genes

Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 microM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run-off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.

scholarly journals Regulation of expression of the neuronal POU protein Oct-2 by nerve growth factor.

1992 ◽  
Vol 267 (25) ◽  
pp. 17787-17791 ◽  
Author(s):  
J.N. Wood ◽  
K.A. Lillycrop ◽  
C.L. Dent ◽  
N.N. Ninkina ◽  
M.M. Beech ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Regulation Of Expression ◽  
Nerve Growth

scholarly journals Natural Antisense Transcript PEBP1P3 Regulates the RNA Expression, DNA Methylation and Histone Modification of CD45 Gene

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 759
Author(s):  
Zhongjing Su ◽  
Guangyu Liu ◽  
Bin Zhang ◽  
Ze Lin ◽  
Dongyang Huang
Keyword(s):  
Dna Methylation ◽  
Alternative Splicing ◽  
Histone Modification ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Antisense Transcript ◽  
Natural Antisense Transcript ◽  
Regulation Of Expression ◽  
Splicing Isoforms ◽  
Intron 2

The leukocyte common antigen CD45 is a transmembrane phosphatase expressed on all nucleated hemopoietic cells, and the expression levels of its splicing isoforms are closely related to the development and function of lymphocytes. PEBP1P3 is a natural antisense transcript from the opposite strand of CD45 intron 2 and is predicted to be a noncoding RNA. The genotype-tissue expression and quantitative PCR data suggested that PEBP1P3 might be involved in the regulation of expression of CD45 splicing isoforms. To explore the regulatory mechanism of PEBP1P3 in CD45 expression, DNA methylation and histone modification were detected by bisulfate sequencing PCR and chromatin immunoprecipitation assays, respectively. The results showed that after the antisense RNA PEBP1P3 was knocked down by RNA interference, the DNA methylation of CD45 intron 2 was decreased and histone H3K9 and H3K36 trimethylation at the alternative splicing exons of CD45 DNA was increased. Knockdown of PEBP1P3 also increased the binding levels of chromatin conformation organizer CTCF at intron 2 and the alternative splicing exons of CD45. The present results indicate that the natural antisense RNA PEBP1P3 regulated the alternative splicing of CD45 RNA, and that might be correlated with the regulation of histone modification and DNA methylation.

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