Remote-Site Stimulation of Secretory IgA Antibodies Following Bronchial and Gastric Stimulation

1978 ◽  
pp. 113-122 ◽  
Author(s):  
Paul C. Montgomery ◽  
Kevin M. Connelly ◽  
Judith Cohn ◽  
Cheryl A. Skandera
Keyword(s):  
Secretory Iga ◽  
Remote Site ◽  
Gastric Stimulation ◽  
Iga Antibodies ◽  
Stimulation Of ◽  
Site Stimulation

Remote-site stimulation of influenza antibodies in monkeys following intestinal stimulation

1986 ◽  
Vol 12 (2-3) ◽  
pp. 65-67 ◽  
Author(s):  
K.-C. Bergmann ◽  
R.H. Waldman ◽  
W. Nordheim ◽  
H.T. Nguyen ◽  
P.L. Huynh ◽  
...  
Keyword(s):  
Remote Site ◽  
Stimulation Of ◽  
Site Stimulation

scholarly journals Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions

2012 ◽  
Vol 19 (10) ◽  
pp. 1593-1596 ◽  
Author(s):  
Ravinder Kaur ◽  
Thomas Kim ◽  
Janet R. Casey ◽  
Michael E. Pichichero
Keyword(s):  
Middle Ear ◽  
Acute Otitis Media ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Two Dimensional Gel Electrophoresis ◽  
Middle Ear Fluid ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Entire Array

ABSTRACTThe human middle ear is devoid of any immunocompetent cells in normal mucosa. We sought to determine the source of antibody present in the middle ear of children. Total IgG, IgA, and secretory IgA antibodies were determined by enzyme-linked immunosorbent assay from the nasopharyngeal, middle ear, and serum samples of children with acute otitis media. The two-dimensional gel electrophoresis pattern of the entire array of IgA antibodies in the nasal wash (NW) and middle ear fluid (MEF) was compared from the MEF and NW samples using isoelectric focusing and Western blotting. The total IgG and IgA antibodies in the MEF and NW samples of 137 children were compared. The ratio of IgG to IgA in the MEF was significantly different (P< 0.008) compared to NW because IgA levels were higher and IgG levels lower in NW. The IgG/IgA ratio of MEF resembled serum consistent with transudation to the MEF. Small amounts of secretory IgA were detected in MEF but the electrophoresis patterns of the entire array of IgA antibodies in the MEF and NW were virtually identical in each child evaluated; thus, IgA in MEF derived predominantly from serum and the nasopharynx by reflux via the Eustachian tube. The IgG/IgA antibody levels in the MEF and the same composition of IgA antibody in the MEF and NW identifies the predominant source of antibody in the MEF as a transudate of serum combined with nasal secretions refluxed from the nasopharynx in children.

Differences in Level and Avidity of Secretory IgA Antibodies in Breast Milk of Swedish, Indian and Japanese Mothers to Soybean Protein

1991 ◽  
Vol 95 (1) ◽  
pp. 13-16 ◽  
Author(s):  
A. Morikawa ◽  
U. Dahlgren ◽  
B. Carlsson ◽  
I. Narayanan ◽  
M. Hahn-Zoric ◽  
...  
Keyword(s):  
Breast Milk ◽  
Soybean Protein ◽  
Secretory Iga ◽  
Iga Antibodies ◽  
Japanese Mothers

The Effects of Acupuncture on White Cell Counts

1974 ◽  
Vol 02 (04) ◽  
pp. 383-398 ◽  
Author(s):  
Marjorie L. Brown ◽  
George A. Ulett ◽  
John A. Stern
Keyword(s):  
Cell Count ◽  
White Cell Count ◽  
Needle Insertion ◽  
White Cell ◽  
Acupuncture Points ◽  
Blood Cell Count ◽  
Cell Counts ◽  
Higher Temperature ◽  
Stimulation Of ◽  
Site Stimulation

The anticipation of acupuncture, simple insertion of needles or the electrical stimulation of needles at both classical acupuncture points and "false" points, all produce an increase in white blood cell count. Electrostimulation produced the greatest, expectation of needle insertion the least, increase in white cell count. Though needles remain to place, the white cell count returns to basal level within one hour. Preliminary data on peripheral skin temperature as affected by stimulation of acupuncture points and non-points, suggests a higher temperature on the side of stimulation. For acupuncture site stimulation, the temperature differential appears to be more persistent than is true when non-sites are stimulated. Subjects reported needle insertion at acupuncture points as less painful than at non-points. Feelings of numbness were produced by stimulation of both classical and false acupuncture points.

scholarly journals Ontogenesis of the formation of secretory antibodies to respiratory syncytial (RS) virus

1988 ◽  
Vol 101 (3) ◽  
pp. 565-575 ◽  
Author(s):  
N. P Leschinskaya ◽  
E. E Pokrovskaya ◽  
E. A Kantorovitch ◽  
S.K Grigorjeva ◽  
YA. S Shvartsman
Keyword(s):  
Old Age ◽  
Serum Antibody ◽  
Close Correlation ◽  
Secretory Iga ◽  
Igg Antibodies ◽  
Virus Infections ◽  
Neutralizing Activity ◽  
Iga Antibodies ◽  
Antibody Levels ◽  
Secretory Antibodies

SUMMARYExamination of sera from 184 children aged between 0 and 12 years and 161 adults revealed a close correlation between age and the level of humoral anti-RS virus immunity. Secretory IgG antibodies were found in children in their first months of life. Evidence for their release into secretions from the serum was obtained. This might explain the positive correlation between serum antibody levels in women recently confined with the morbidity due to RS virus in children during their first months of life. Secretory IgA antibodies were found from 4 months untill old age. The secretions of children and adults contained virus-neutralizing activity which was non-immunoglobulin in nature, as well as antibodies. However, in contrast to secretory antibody this material did not prevent development of severe RS virus infections.

HLAMYDIAL SECRETORY IgA ANTIBODIES IN HUMAN MILK

2009 ◽  
Vol 90C (1-6) ◽  
pp. 21-25
Author(s):  
Kjell Skaug ◽  
Anne-Brit Otnaess ◽  
Ivar ørstavik ◽  
Fridtjof Jerve
Keyword(s):  
Human Milk ◽  
Secretory Iga ◽  
Iga Antibodies

scholarly journals Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

2013 ◽  
Vol 79 (13) ◽  
pp. 3917-3925 ◽  
Author(s):  
James A. Gregory ◽  
Aaron B. Topol ◽  
David Z. Doerner ◽  
Stephen Mayfield
Keyword(s):  
Cholera Toxin ◽  
Tandem Repeats ◽  
Low Cost ◽  
Oral Vaccine ◽  
Childhood Mortality ◽  
Secretory Iga ◽  
Content Type ◽  
Freeze Dried ◽  
Vaccine Antigens ◽  
Iga Antibodies

ABSTRACTInfectious diseases disproportionately affect indigent regions and are the greatest cause of childhood mortality in developing countries. Practical, low-cost vaccines for use in these countries are paramount to reducing disease burdens and concomitant poverty. Algae are a promising low-cost system for producing vaccines that can be orally delivered, thereby avoiding expensive purification and injectable delivery. We engineered the chloroplast of the eukaryotic algaChlamydomonas reinhardtiito produce a chimeric protein consisting of the 25-kDaPlasmodium falciparumsurface protein (Pfs25) fused to the β subunit of the cholera toxin (CtxB) to investigate an alga-based whole-cell oral vaccine. Pfs25 is a promising malaria transmission-blocking vaccine candidate that has been difficult to produce in traditional recombinant systems due to its structurally complex tandem repeats of epidermal growth factor-like domains. The noncatalytic CtxB domain of the cholera holotoxin assembles into a pentameric structure and acts as a mucosal adjuvant by binding GM1 ganglioside receptors on gut epithelial cells. We demonstrate that CtxB-Pfs25 accumulates as a soluble, properly folded and functional protein within algal chloroplasts, and it is stable in freeze-dried alga cells at ambient temperatures. In mice, oral vaccination using freeze-dried algae that produce CtxB-Pfs25 elicited CtxB-specific serum IgG antibodies and both CtxB- and Pfs25-specific secretory IgA antibodies. These data suggest that algae are a promising system for production and oral delivery of vaccine antigens, but as an orally delivered adjuvant, CtxB is best suited for eliciting secretory IgA antibodies for vaccine antigens against pathogens that invade mucosal surfaces using this strategy.

Sign in / Sign up

Export Citation Format

Share Document