Mechanism of HVJ-induced Cell Fusion

1982 ◽  
pp. 57-78 ◽  
Author(s):  
Akira Asano ◽  
Kimiko Asano
Keyword(s):  
Cell Fusion

Cell Fusion is a Potent Inducer of Aneuploidy and Drug Resistance in Tumor Cell/ Normal Cell Hybrids

2013 ◽  
Vol 18 (1 - 2) ◽  
pp. 97-113 ◽  
Author(s):  
Benjamin Berndt ◽  
Kurt S. Zanker ◽  
Thomas Dittmar
Keyword(s):  
Drug Resistance ◽  
Tumor Cell ◽  
Cell Fusion ◽  
Normal Cell ◽  
Potent Inducer ◽  
Cell Hybrids

The Dark Side of Stem Cells: Triggering Cancer Progression by Cell Fusion

2013 ◽  
Vol 13 (5) ◽  
pp. 735-750 ◽  
Author(s):  
T. Dittmar ◽  
C. Nagler ◽  
B. Niggemann ◽  
K.S. Zanker
Keyword(s):  
Stem Cells ◽  
Cell Fusion ◽  
Cancer Progression ◽  
Dark Side

scholarly journals Acquisition of Cell–Cell Fusion Activity by Amino Acid Substitutions in Spike Protein Determines the Infectivity of a Coronavirus in Cultured Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (7) ◽  
pp. e6130 ◽  
Author(s):  
Yoshiyuki Yamada ◽  
Xiao Bo Liu ◽  
Shou Guo Fang ◽  
Felicia P. L. Tay ◽  
Ding Xiang Liu
Keyword(s):  
Amino Acid ◽  
Cell Fusion ◽  
Cultured Cells ◽  
Spike Protein ◽  
Amino Acid Substitutions ◽  
Fusion Activity ◽  
Cell Cell

scholarly journals Weak Electromagnetic Fields Accelerate Fusion of Myoblasts

2021 ◽  
Vol 22 (9) ◽  
pp. 4407
Author(s):  
Dana Adler ◽  
Zehavit Shapira ◽  
Shimon Weiss ◽  
Asher Shainberg ◽  
Abram Katz
Keyword(s):  
Skeletal Muscle ◽  
Cell Membrane ◽  
Cell Fusion ◽  
Electromagnetic Fields ◽  
Muscle Development ◽  
K Channel ◽  
Gambogic Acid ◽  
Cell Replication ◽  
Myotube Formation ◽  
Primary Myoblast

Weak electromagnetic fields (WEF) alter Ca2+ handling in skeletal muscle myotubes. Owing to the involvement of Ca2+ in muscle development, we investigated whether WEF affects fusion of myoblasts in culture. Rat primary myoblast cultures were exposed to WEF (1.75 µT, 16 Hz) for up to six days. Under control conditions, cell fusion and creatine kinase (CK) activity increased in parallel and peaked at 4–6 days. WEF enhanced the extent of fusion after one and two days (by ~40%) vs. control, but not thereafter. Exposure to WEF also enhanced CK activity after two days (almost four-fold), but not afterwards. Incorporation of 3H-thymidine into DNA was enhanced by one-day exposure to WEF (~40%), indicating increased cell replication. Using the potentiometric fluorescent dye di-8-ANEPPS, we found that exposure of cells to 150 mM KCl resulted in depolarization of the cell membrane. However, prior exposure of cells to WEF for one day followed by addition of KCl resulted in hyperpolarization of the cell membrane. Acute exposure of cells to WEF also resulted in hyperpolarization of the cell membrane. Twenty-four hour incubation of myoblasts with gambogic acid, an inhibitor of the inward rectifying K+ channel 2.1 (Kir2.1), did not affect cell fusion, WEF-mediated acceleration of fusion or hyperpolarization. These data demonstrate that WEF accelerates fusion of myoblasts, resulting in myotube formation. The WEF effect is associated with hyperpolarization but WEF does not appear to mediate its effects on fusion by activating Kir2.1 channels.

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