Lipid Peroxidation, Protein Thiols and Calcium Homeostasis in Bromobenzene-Induced Liver Damage

1988 ◽  
pp. 773-776 ◽  
Author(s):  
A. F. Casini ◽  
E. Maellaro ◽  
A. Pompella ◽  
M. Ferrali ◽  
M. Comporti
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Calcium Homeostasis ◽  
Protein Thiols

Lipid Peroxidation, Protein Thiols, Calcium Homeostasis and Imbalance of Antioxidant Systems in Bromobenzene Induced Liver Damage

1988 ◽  
pp. 153-163
Author(s):  
Alessandro F. Casini ◽  
Emilia Maellaro ◽  
Alfonso Pompella ◽  
Marco Ferrali ◽  
Mario Comporti
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Calcium Homeostasis ◽  
Antioxidant Systems ◽  
Protein Thiols

Lipid peroxidation, protein thiols and calcium homeostasis in bromobenzene-induced liver damage

1987 ◽  
Vol 36 (21) ◽  
pp. 3689-3695 ◽  
Author(s):  
Alessandro F. Casini ◽  
Emilia Maellaro ◽  
Alfonso Pompella ◽  
Marco Ferrali ◽  
Mario Comporti
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Calcium Homeostasis ◽  
Protein Thiols

scholarly journals Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2391 ◽  
Author(s):  
Rafael Sari ◽  
Paula Conterno ◽  
Leticia Dangui da Silva ◽  
Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
High Performance ◽  
Principal Component ◽  
Antioxidant Defense System ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Antioxidant Enzyme Activities ◽  
Protein Thiols ◽  
Liver And Kidney

The aim of this study was to evaluate the most effective extraction condition (temperature, solvent type and time) for recovery of high-value phytochemicals present in the Tabernaemontana catharinensis leaves (TC) and to assess their effect on biochemical parameters in streptozotocin-induced diabetic rats. The extraction of phenolic compounds from TC using a factorial design (FD) 2³, high performance liquid chromatography (HPLC), response surface methodology (RSM) and principal component analysis (PCA) were studied. It was found that the optimal conditions for extraction of phenolics were higher temperature (65 °C) and time (60 min) using ethanol as extractor solvent. In this condition of extraction (A8), total phenolic compounds (TPC) and antioxidant activity (AA) were determined. Additionally, this extract was used to evaluate their effect on antioxidant enzyme activities (superoxide dismutase (SOD) and catalase (CAT)) as well as lipid peroxidation (LP) and protein thiols level (PSH) in the liver and kidneys of normal and diabetic rats. As result, T. catharinensis extract presented TPC content of 23.34 mg EAG/g (equivalent gallic acid) and AA of 34.26 μmol Trolox/g. Phenolic acids (ferulic acid and coumaric acid) and flavonoids (quercetin, rutin and pinocembrin) could be recovered and identified by HPLC. This study indicated an important role of the T. catharinensis extract on free radical inactivation and on the antioxidant defense system in diabetic rats. In fact, the use of T. catharinensis extract restored the normal activity of SOD (p < 0.05) and suppressed malondialdehyde levels in liver and kidney tissues. Thus, the T. catharinensis extract, rich in phenolic compounds, can be responsible for the recover the enzymatic changes in the liver and kidney tissues provoked by diabetes in rats. In addition, the lipid peroxidation rate decreased in the diabetic rats treated with T. catharinensis.

scholarly journals CHEMICAL COMPOSITION AND HEPATOPROTECTIVE EFFECTS OF POLYPHENOLIC FRACTION FROM RIVEA HYPOCRATERIFORMIS IN PARACETAMOL INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS

2016 ◽  
Vol 8 (10) ◽  
pp. 228
Author(s):  
Shivakumar S. Godipurge ◽  
Naveen J. Biradar ◽  
Jaiprakash S. Biradar ◽  
Nitin Mahurkar
Keyword(s):  
Lipid Peroxidation ◽  
Chemical Composition ◽  
Hydroxyl Radical ◽  
Liver Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Aerial Parts ◽  
Hepatoprotective Effects ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>This study was designed to chemical composition and hepatoprotective effects of a polyphenolic fraction of aerial parts of <em>R. hypocrateriformis </em>(PPFRH). It was shown to exhibit strong <em>in vitro</em> lipid peroxidation and scavenging activity against hydroxyl radical.</p><p><strong>Methods: </strong>The chemical composition of a polyphenolic fraction of <em>R. hypocrateriformis</em> was analyzed by High-performance liquid chromatography method. Hepatocellular injuries induced by paracetamol were assessed by liver damage in Wistar albino rat; the hepatoprotective effect was evaluated by biochemical parameters in rat serum, antioxidant hydroxyl radical scavenging activity and lipid peroxidation in liver tissue.</p><p><strong>Results: </strong>The polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> for lipid peroxidation is significantly (p&lt;0.05). In the hepatoprotective activity of liver enzymes and hepatic necrosis were significantly (p&lt;0.001) closer to paracetamol. The correlation coefficient between the hydroxyl scavenging radical and total phenolic and flavonoid contents were found to be R² = 0.9045 and R² = 0.8876 suggesting the contribution of phenolic and flavonoid compounds of the polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> by 90% and 88% to its radical scavenging activity.</p><p><strong>Conclusion: </strong>The polyphenolic fraction of aerial parts of <em>R. hypocrateriformis</em> possesses a significant protective effect against acute hepatotoxicity induced by paracetamol and which may be due to the phenolic and flavonoid components.</p>

Lipid peroxidation and antioxidants status in human malignant and non-malignant thyroid tumours

2015 ◽  
Vol 35 (6) ◽  
pp. 585-597 ◽  
Author(s):  
JA Stanley ◽  
R Neelamohan ◽  
E Suthagar ◽  
G Vengatesh ◽  
J Jayakumar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Reduced Glutathione ◽  
Human Thyroid ◽  
Malignant Tumours ◽  
Nodular Goitre ◽  
Hormone Synthesis ◽  
Stress Markers ◽  
Follicular Thyroid Adenoma ◽  
Protein Thiols ◽  
Design And Methods

Introduction: Thyroid epithelial cells produce moderate amounts of reactive oxygen species that are physiologically required for thyroid hormone synthesis. Nevertheless, when they are produced in excessive amounts, they may become toxic. Objective: The present study is aimed to compare the lipid peroxidation (LPO), antioxidant enzymes – superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and non-protein thiols (reduced glutathione (GSH)) in human thyroid tissues with malignant and non-malignant disorders. Design and Methods: The study used human thyroid tissues and blood samples from 157 women (147 diseased and 10 normal). Thyroid hormones, oxidative stress markers and antioxidants were estimated by standard methods. Results: LPO significantly increased in most of the papillary thyroid carcinoma (PTC: 82.9%) and follicular thyroid adenoma (FTA: 72.9%) tissues, whilst in a majority of nodular goitre (69.2%) and Hashimoto’s thyroiditis (HT: 73.7%) thyroid tissues, it remained unaltered. GSH increased in PTC (55.3%), remained unaltered in FTA (97.3%) and all other goiter samples studied. SOD increased in PTC (51.1%) and all other malignant thyroid tissues studied. CAT remained unaltered in PTC (95.7%), FTA (97.3%) and all other non-malignant samples (HT, MNG, TMNG) studied. GPx increased in PTC (63.8%), all other malignant thyroid tissues and remained unaltered in many of the FTA (91.9%) tissues and all other non-malignant samples (HT, MNG, TMNG) studied. Conclusions: In the case of non-malignant thyroid tumours, the oxidant–antioxidant balance was undisturbed, whilst in malignant tumours the balance was altered, and the change in r value observed in the LPO and SOD pairs between normal and PTC tissues and also in many pairs with multi-nodular goitre (MNG)/toxic MNG tissues may be used as a marker to differentiate/detect different malignant/non-malignant thyroid tumours.

Anti-hepatotoxic effects of 3,4-methylenedioxyphenol and N-acetylcysteine in acutely acetaminophen-overdosed mice

2011 ◽  
Vol 30 (10) ◽  
pp. 1609-1615 ◽  
Author(s):  
Victor Raj Mohan Chandrasekaran ◽  
Se-Ping Chien ◽  
Dur-Zong Hsu ◽  
Ming-Yie Liu
Keyword(s):  
Lipid Peroxidation ◽  
Liver Injury ◽  
Protective Effect ◽  
Liver Damage ◽  
Alanine Transaminase ◽  
Aspartate Transaminase ◽  
Hepatic Glutathione ◽  
Hepatotoxic Effect

3,4-Methylenedioxyphenol (sesamol) is effective against acetaminophen-induced liver injury in rats. Whether sesamol’s anti-hepatotoxic effect is comparable to that of N-acetylcysteine has never been studied. We investigated the anti-hepatotoxic effects of sesamol and N-acetylcysteine on acetaminophen-induced hepatotoxicity in mice. Equimolar doses (1 mmol/kg) of sesamol and N-acetylcysteine significantly inhibited acetaminophen (300 mg/kg)-increased serum aspartate transaminase and alanine transaminase levels 6 h post-administration. Sesamol and N-acetylcysteine maintained hepatic glutathione levels and inhibited lipid peroxidation. Moreover, the combination of sesamol and N-acetylcysteine antagonistically inhibited sesamol’s protection against acetaminophen-induced liver injury. We conclude that the protective effect of sesamol against acetaminophen-induced liver damage is comparable to that of N-acetylcysteine by maintaining glutathione levels and inhibiting lipid peroxidation in mice.

scholarly journals The comparative evaluation of plant adaptogenes’ hepatoprotective properties on the experimental model of rat liver chronic alcohol dama ge in vivo

2019 ◽  
pp. 54-62 ◽  
Author(s):  
Aksana I. Hubich ◽  
Yana Yu. Dashkova ◽  
Ina N. Krivlenya
Keyword(s):  
Lipid Peroxidation ◽  
Liver Damage ◽  
Unsaturated Fatty Acids ◽  
Alcohol Intoxication ◽  
Liver Homogenate ◽  
Chronic Alcohol ◽  
Laboratory Rats ◽  
Plant Adaptogens ◽  
Damage Indicators

This work is devoted to the study of the effectiveness of biochemical liver damage markers and lipid peroxidation correction by plant adaptogens (Sideritis scardica, Hibiscus sabdariffa, Rhododendron adamsii Rehder) in laboratory rats with experimental chronic alcohol intoxication. It was shown that the using of all drugs studied contributes to the normalization of liver damage indicators (alkaline phosphatase and alanine aminotransferase activity, the content of free and bound bilirubin in the blood serum) and lipid peroxidation markers (the content of reduced SH-groups and TBA-active products, catalase and superoxide dismutase activity in the liver homogenate). It was demonstrated that the most pronounced antioxidant and hepatoprotective effects in this model, comparable to the action of classical hepatoprotectors «Hepsil-Rn» and «Essentiale Forte», had decoctions of Sideritis scardica and Rhododendron adamsii Rehder which may be due to the high content of flavonoids and unsaturated fatty acids in their composition.

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