Conservation of Signal: A New Algorithm for the Elimination of the Reference Concentration as an Independently Variable Parameter in the Analysis of Sedimentation Equilibrium

Author(s):  
Allen P. Minton
1970 ◽  
Vol 24 (03/04) ◽  
pp. 325-333 ◽  
Author(s):  
G. H Tishkoff ◽  
L. C Williams ◽  
D. M Brown

SummaryAs a corollary to our previous studies with bovine prothrombin, we have initiated a study of human prothrombin complex. This product has been isolated in crystalline form as a barium glycoprotein interaction product. Product yields were reduced compared to bovine product due to the increased solubility of the barium glycoprotein interaction product. On occasion the crystalline complex exhibited good yields. The specific activity of the crystalline complex was 1851 Iowa u/mg. Further purification of human prothrombin complex was made by removal of barium and by chromatography on Sephadex G-100 gels. The final product evidenced multiple procoagulant activities (II, VII, IX and X). The monomeric molecular weight determined by sedimentation equilibrium in a solvent of 6 M guanidine-HCl and 0.5% mercaptoethanol was 70,191 ± 3,057 and was homogeneous with respect to molecular weight. This product was characterized in regard to physical constants and chemical composition. In general, the molecular properties of human prothrombin complex are very similar to the comparable bovine product. In some preparations a reversible proteolytic enzyme inhibitor (p-aminophenylarsonic acid) was employed in the ultrafiltration step of the purification scheme to inhibit protein degradation.


2001 ◽  
Vol 29 (4) ◽  
pp. 258-268 ◽  
Author(s):  
G. Jianmin ◽  
R. Gall ◽  
W. Zuomin

Abstract A variable parameter model to study dynamic tire responses is presented. A modified device to measure terrain roughness is used to measure dynamic damping and stiffness characteristics of rolling tires. The device was used to examine the dynamic behavior of a tire in the speed range from 0 to 10 km/h. The inflation pressure during the tests was adjusted to 160, 240, and 320 kPa. The vertical load was 5.2 kN. The results indicate that the damping and stiffness decrease with velocity. Regression formulas for the non-linear experimental damping and stiffness are obtained. These results can be used as input parameters for vehicle simulation to evaluate the vehicle's driving and comfort performance in the medium-low frequency range (0–100 Hz). This way it can be important for tire design and the forecasting of the dynamic behavior of tires.


Machines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 67
Author(s):  
Laixi Zhang ◽  
Chenming Zhao ◽  
Feng Qian ◽  
Jaspreet Singh Dhupia ◽  
Mingliang Wu

Vibrations in the aircraft assembly building will affect the precision of the robotic drilling system. A variable stiffness and damping semiactive vibration control mechanism with quasi-zero stiffness characteristics is developed. The quasi-zero stiffness of the mechanism is realized by the parallel connection of four vertically arranged bearing springs and two symmetrical horizontally arranged negative stiffness elements. Firstly, the quasi-zero stiffness parameters of the mechanism at the static equilibrium position are obtained through analysis. Secondly, the harmonic balance method is used to deal with the differential equations of motion. The effects of every parameter on the displacement transmissibility are analyzed, and the variable parameter control strategies are proposed. Finally, the system responses of the passive and semiactive vibration isolation mechanisms to the segmental variable frequency excitations are compared through virtual prototype experiments. The results show that the frequency range of vibration isolation is widened, and the stability of the vibration control system is effectively improved without resonance through the semiactive vibration control method. It is of innovative significance for ambient vibration control in robotic drilling systems.


Coatings ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 570
Author(s):  
Olga Sánchez ◽  
Manuel Hernández-Vélez

ZnOTe compounds were grown by DC magnetron cosputtering from pure Tellurium (Te) and Zinc (Zn) cathodes in O2/Ar atmosphere. The applied power on the Zn target was constant equal to 100 W, while the one applied on the Te target took two values, i.e., 5 W and 10 W. Thus, two sample series were obtained in which the variable parameter was the distance from the Te targets to the substrate. Sample compositions were determined by Rutherford Backscattering Spectroscopy (RBS) experiments. Structural analysis was done using X-Ray diffraction (XRD) spectrometry and the growth of the hexagonal w-ZnO phase was identified in the XRD spectra. RBS results showed high bulk homogeneity of the samples forming ZnOTe alloys, with variable Te molar fraction (MF) ranging from 0.48–0.6% and from 1.9–3.1% for the sample series obtained at 5 W and 10 W, respectively. The results reflect great differences between the two sample series, particularly from the structural and optical point of view. These experiments point to the possibility of Te doping ZnO with the permanence of intrinsic defects, as well as the possibility of the formation of other Te solid phases when its content increases. The results and appreciable variations in the band gap transitions were detected from Photoluminescence (PL) measurements.


Author(s):  
Xiang Ma ◽  
Xuemei Li ◽  
Yuanfeng Zhou ◽  
Caiming Zhang

AbstractSmoothing images, especially with rich texture, is an important problem in computer vision. Obtaining an ideal result is difficult due to complexity, irregularity, and anisotropicity of the texture. Besides, some properties are shared by the texture and the structure in an image. It is a hard compromise to retain structure and simultaneously remove texture. To create an ideal algorithm for image smoothing, we face three problems. For images with rich textures, the smoothing effect should be enhanced. We should overcome inconsistency of smoothing results in different parts of the image. It is necessary to create a method to evaluate the smoothing effect. We apply texture pre-removal based on global sparse decomposition with a variable smoothing parameter to solve the first two problems. A parametric surface constructed by an improved Bessel method is used to determine the smoothing parameter. Three evaluation measures: edge integrity rate, texture removal rate, and gradient value distribution are proposed to cope with the third problem. We use the alternating direction method of multipliers to complete the whole algorithm and obtain the results. Experiments show that our algorithm is better than existing algorithms both visually and quantitatively. We also demonstrate our method’s ability in other applications such as clip-art compression artifact removal and content-aware image manipulation.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Michelle Cutajar ◽  
Fabrizio Andriulo ◽  
Megan R. Thomsett ◽  
Jonathan C. Moore ◽  
Benoit Couturaud ◽  
...  

AbstractThere is currently a pressing need for the development of novel bioinspired consolidants for waterlogged, archaeological wood. Bioinspired materials possess many advantages, such as biocompatibility and sustainability, which makes them ideal to use in this capacity. Based on this, a polyhydroxylated monomer was synthesised from α-pinene, a sustainable terpene feedstock derived from pine trees, and used to prepare a low molar mass polymer TPA5 through free radical polymerisation. This polymer was extensively characterised by NMR spectroscopy (chemical composition) and molecular hydrodynamics, primarily using analytical ultracentrifugation reinforced by gel filtration chromatography and viscometry, in order to investigate whether it would be suitable for wood consolidation purposes. Sedimentation equilibrium indicated a weight average molar mass Mw of (4.3 ± 0.2) kDa, with minimal concentration dependence. Further analysis with MULTISIG revealed a broad distribution of molar masses and this heterogeneity was further confirmed by sedimentation velocity. Conformation analyses with the Perrin P and viscosity increment ν universal hydrodynamic parameters indicated that the polymer had an elongated shape, with both factors giving consistent results and a consensus axial ratio of ~ 4.5. These collective properties—hydrogen bonding potential enhanced by an elongated shape, together with a small injectable molar mass—suggest this polymer is worthy of further consideration as a potential consolidant.


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