Cryogenic Cooling of Biological Samples for Electron and Optical Microscopy

1991 ◽  
pp. 117-128
Author(s):  
G. J. Fuld ◽  
R. G. Hansen ◽  
N. Loes
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Cryogenic Cooling

A labeling strategy with effective preservation of fluorophores for expansion single molecule localization microscopy(Ex-SMLM)

The Analyst ◽  
2021 ◽  
Author(s):  
Weibing Kuang ◽  
Bo Xin ◽  
Zhen-li Huang ◽  
Bing Shi
Keyword(s):  
Optical Microscopy ◽  
Single Molecule ◽  
Biological Samples ◽  
Localization Microscopy ◽  
Single Molecule Localization Microscopy

Expansion Microscopy (ExM) significantly improves the resolution of conventional diffraction-limited optical microscopy by using physically expanding biological samples. Combining ExM with single molecule localization microscopy (SMLM) could further enhance the...

Development of a Combined Scanning Ion-Conductance and Nearfield Optical Microscope to Image Living Cells

1999 ◽  
Vol 5 (S2) ◽  
pp. 976-977
Author(s):  
M. Raval ◽  
D. Klenerman ◽  
T. Rayment ◽  
Y. Korchev ◽  
M. Lab
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Near Field ◽  
Sample Surface ◽  
Optical Microscope ◽  
Ion Conductance ◽  
Simultaneous Generation ◽  
Simple Arrangement ◽  
Wavelength Resolution ◽  
Sub Wavelength

It is important to be able to image biological samples in a manner that is non-invasive and allows the sample to retain its functionality during imaging.A member of the SPM (scanning probe microscopy) family, SNOM (scanning near-field optical microscopy), has emerged as a technique that allows optical and topographic imaging of biological samples whilst satisfying the above stated criteria. The basic operating principle of SNOM is as follows. Light is coupled down a fibre-optic probe with an output aperture of sub-wavelength dimensions. The probe is then scanned over the sample surface from a distance that is approximately equal to the size of its aperture. By this apparently simple arrangement, the diffraction limit posed by conventional optical microscopy is overcome and simultaneous generation of optical and topographic images of sub-wavelength resolution is made possible. Spatial resolution values of lOOnm in air and 60nm in liquid[1,2] are achievable with SNOM.

scholarly journals Multimodal Nonlinear Optical Microscopy for Simultaneous 3-D Label-Free and Immunofluorescence Imaging of Biological Samples

2014 ◽  
Vol 18 (5) ◽  
pp. 551-557
Author(s):  
Joo Hyun Park ◽  
Eun-Soo Lee ◽  
Jae Yong Lee ◽  
Eun Seong Lee ◽  
Tae Geol Lee ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Nonlinear Optical ◽  
Label Free ◽  
Nonlinear Optical Microscopy

scholarly journals A beginner’s guide to improving image acquisition in fluorescence microscopy

2020 ◽  
Vol 42 (6) ◽  
pp. 22-27
Author(s):  
Takeo Ogama
Keyword(s):  
Fluorescence Microscopy ◽  
Optical Microscopy ◽  
Biological Samples ◽  
Image Acquisition ◽  
Digital Data ◽  
Ideal Image ◽  
The Ideal

This article presents an overview of optical microscopy and digs into the details of fluorescence microscopy, exploring the link between the signals in biological samples and the digital data from microscope cameras. Understanding this relationship can help you set the ideal image acquisition conditions to achieve the highest quality images and data.

Containers for uniform hyper-fast cryogenic cooling of thick biological samples

Cryobiology ◽  
2018 ◽  
Vol 80 ◽  
pp. 187
Author(s):  
Vladimir F. Bolyukh ◽  
Igor Katkov
Keyword(s):  
Biological Samples ◽  
Cryogenic Cooling

Active bimorph-based tapping-mode distance control for scanning near-field optical microscopy of biological samples in liquid

2006 ◽  
Vol 100 (8) ◽  
pp. 084317 ◽  
Author(s):  
F. H. Lei ◽  
L. Huang ◽  
O. Piot ◽  
A. Trussardi ◽  
M. Manfait ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Near Field ◽  
Tapping Mode ◽  
Distance Control

scholarly journals Exploring the cellular and tissue uptake of nanomaterials in a range of biological samples using multimodal nonlinear optical microscopy

2015 ◽  
Vol 26 (50) ◽  
pp. 505102 ◽  
Author(s):  
Helinor J Johnston ◽  
Rabah Mouras ◽  
David M Brown ◽  
Alistair Elfick ◽  
Vicki Stone
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Nonlinear Optical ◽  
Tissue Uptake ◽  
Nonlinear Optical Microscopy
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