scholarly journals A beginner’s guide to improving image acquisition in fluorescence microscopy

2020 ◽  
Vol 42 (6) ◽  
pp. 22-27
Author(s):  
Takeo Ogama

This article presents an overview of optical microscopy and digs into the details of fluorescence microscopy, exploring the link between the signals in biological samples and the digital data from microscope cameras. Understanding this relationship can help you set the ideal image acquisition conditions to achieve the highest quality images and data.

Author(s):  
Brian Cross

A relatively new entry, in the field of microscopy, is the Scanning X-Ray Fluorescence Microscope (SXRFM). Using this type of instrument (e.g. Kevex Omicron X-ray Microprobe), one can obtain multiple elemental x-ray images, from the analysis of materials which show heterogeneity. The SXRFM obtains images by collimating an x-ray beam (e.g. 100 μm diameter), and then scanning the sample with a high-speed x-y stage. To speed up the image acquisition, data is acquired "on-the-fly" by slew-scanning the stage along the x-axis, like a TV or SEM scan. To reduce the overhead from "fly-back," the images can be acquired by bi-directional scanning of the x-axis. This results in very little overhead with the re-positioning of the sample stage. The image acquisition rate is dominated by the x-ray acquisition rate. Therefore, the total x-ray image acquisition rate, using the SXRFM, is very comparable to an SEM. Although the x-ray spatial resolution of the SXRFM is worse than an SEM (say 100 vs. 2 μm), there are several other advantages.


Author(s):  
M.V. SAFONOVA ◽  
◽  
M.A. KOSINOVA ◽  
E.A. ROMANOVA ◽  
◽  
...  

Statement of the problem. Solving the problem of psychological and pedagogical support for parents requires studying the request for psychological assistance from their side. In our opinion, this can be done by studying social ideas of modern parents about various aspects of the parenthood phenomenon. The purpose of the article is to present a comparative analysis of semantic cores of ideas about parenthood in adults with and without children. Materials and methods. The research methodology is represented by the concept of social representations by S. Moscovici, as well as generalized studies in the field of social representations by Russian and foreign scientists (K. A. Abulkhanova-Slavskaya, G. M. Andreeva, E. Yu. Artemyeva, O. E. Baskansky, I. B. Bovina, M. I. Volovikova, T. P. Emelyanova, I. Markova, L. G. Pochebut, P. N. Shikhirev, E. V. Yakimova, J. K. Abrik, P. Verges, D. Jodle). Research results. The core of ideas about parenting in adults with and without children are concepts that reflect emotional and behavioral components of parenthood. According to Russian adults who do not have children, parenthood is a responsible and very complex phenomenon, and this can make them postpone child birth. In the minds of potential parents, there is a fairly idealized image of a parent who has remarkable personal qualities, is understanding, caring, and knows how to raise a model child. This largely explains their references to “not being ready” to be a parent, as well as subsequent dissatisfaction with the experience of parenthood, when the ideal image does not correspond to reality. Conclusion. Differences in understanding the concept of parenting in people who have and do not have children clearly describe the crisis experienced by parents when their expectations of parenthood do not meet the reality in which the parental role is performed. In this regard, programs of psychological preparation for parenthood are important, as well as psychological and pedagogical support for young parents in the process of developing their parental competence.


2021 ◽  
Vol 62 (2) ◽  
pp. 473-503
Author(s):  
Korinna Schönhärl

Abstract From the 1880s scientists developed methods to measure (dishonest) tax payment behaviour. The first part of this article provides an overview of these methods and their development. The second part enquires into the function of measuring methods in the societal discourse about (honest) tax payments. The tax morale research of Günter Schmölders, carried out in the 1950s and 1960s, is then examined as a case study. The focus of interest is on the political advice that Schmölders gave, as based on his empirical results, and on the ideal image of the citizen and society which underlay the scientific method.


The Analyst ◽  
2021 ◽  
Author(s):  
Weibing Kuang ◽  
Bo Xin ◽  
Zhen-li Huang ◽  
Bing Shi

Expansion Microscopy (ExM) significantly improves the resolution of conventional diffraction-limited optical microscopy by using physically expanding biological samples. Combining ExM with single molecule localization microscopy (SMLM) could further enhance the...


1999 ◽  
Vol 5 (S2) ◽  
pp. 976-977
Author(s):  
M. Raval ◽  
D. Klenerman ◽  
T. Rayment ◽  
Y. Korchev ◽  
M. Lab

It is important to be able to image biological samples in a manner that is non-invasive and allows the sample to retain its functionality during imaging.A member of the SPM (scanning probe microscopy) family, SNOM (scanning near-field optical microscopy), has emerged as a technique that allows optical and topographic imaging of biological samples whilst satisfying the above stated criteria. The basic operating principle of SNOM is as follows. Light is coupled down a fibre-optic probe with an output aperture of sub-wavelength dimensions. The probe is then scanned over the sample surface from a distance that is approximately equal to the size of its aperture. By this apparently simple arrangement, the diffraction limit posed by conventional optical microscopy is overcome and simultaneous generation of optical and topographic images of sub-wavelength resolution is made possible. Spatial resolution values of lOOnm in air and 60nm in liquid[1,2] are achievable with SNOM.


Designs ◽  
2019 ◽  
Vol 3 (3) ◽  
pp. 35
Author(s):  
Yeh Po-Chan

Body function begins to decline in middle age, with changes becoming increasingly noticeable over time. With the popularization of educational and information technology, people know more about healthcare and are becoming accustomed to self-testing using health equipment. Technological changes are reflected in products, which present innovations including the switch from traditional to touch-controlled interface designs. This can cause difficulties in the interpretation and interface operation for older adults, who may be facing physiological and psychological alterations. Understanding users’ physiological limitations has become an important aspect of product design. This study explored the effects of physiological limitations on touch-screen operation in middle-aged and elderly people, specifically regarding button type, display position, and button size. A total of 64 participants were included in the study: 32 middle-aged people (aged 45–64 years) and 32 elderly people (65 years of age and older). Each participant was asked to complete 32 tasks (two button categories × four button sizes × four presentation positions). The results revealed no differences between the elderly and the middle-aged groups with regard to the interpretation of image buttons and text buttons; however, button size affected the operation and interpretation time. Middle-aged participants demonstrated good interpretation performance when the buttons were displayed in the upper or lower part of the screen, whereas elderly participants only had a good interpretation performance when the buttons were in the upper part. For both groups, the ideal image button size was 16 mm with a text font size of 22.


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