Analysis of Components of Aspergillus and Neosartorya Mycelial Preparations by Gel Electrophoresis and Western Blotting Procedures

1990 ◽  
pp. 235-247 ◽  
Author(s):  
V. M. Hearn ◽  
M. Moutaouakil ◽  
J.-P. Latgé
Keyword(s):  
Western Blotting ◽  
Gel Electrophoresis

Variation in ‘target’ antigens between appropriate and inappropriate hosts of Taenia saginata metacestodes

Parasitology ◽  
1984 ◽  
Vol 88 (4) ◽  
pp. 659-663 ◽  
Author(s):  
L. J. S. Harrison ◽  
R. M. E. Parkhouse ◽  
M. M. H. Sewell
Keyword(s):  
Western Blotting ◽  
Host Resistance ◽  
Gel Electrophoresis ◽  
Parasitic Infection ◽  
Humoral Response ◽  
Challenge Infection ◽  
Infected Cattle ◽  
Taenia Saginata ◽  
Potential Value ◽  
Susceptible Group

SUMMARYThe antibody response of the appropriate hosts (cattle) to Taenia saginata larvae was compared with that of an inappropriate host (Balb/c mice) using gel electrophoresis followed by immunoelectrotransfer blot techniques (Western blotting). Three groups of cattle were included, those known to be resistant to challenge infection because of repeated oral challenge with T. saginata eggs over a 1-year period, a previously infected but known susceptible group and a group of uninfected controls. Serum from the mice and the two groups of infected cattle contained antibodies recognizing different ‘target’ antigens, some of which may be related to host resistance. The potential value of the technique of Western blotting in dissecting the humoral response of a particular host species to parasitic infection and in the identification of those antigens suitable for the production of effective vaccines is discussed.

Ochratoxin a reduces NMDA receptor subunits 2A and 2B concentrations in rat hippocampus: partial protective effect of melatonin

2003 ◽  
Vol 22 (6) ◽  
pp. 335-339 ◽  
Author(s):  
Namik Delibas ◽  
Irfan Altuntas ◽  
Zafer Yonden ◽  
Nurten Ozcelik
Keyword(s):  
Nmda Receptor ◽  
Protective Effect ◽  
Ochratoxin A ◽  
Western Blotting ◽  
Cognitive Functions ◽  
Gel Electrophoresis ◽  
Learning Processes ◽  
Control Group ◽  
Nmda Receptor Subunits ◽  
The Brain

Ochratoxin A (OTA) is a mycotoxin produced by several fungi. Many foods can be contaminated by OTA, which is consequently found in the blood of humans and animals. It is known that OTA accumulates in the brain. The aim of this study was to investigate the effects of OTA on the brain. For this purpose, the effect of OTA on N-methyl-Daspartate (NMDA) receptor subunits 2A (NR2A) and 2B (NR2B) in the hippocampus and the protective effect of melatonin were investigated. Three groups of eight rats were used: controls, OTA-treated rats (OTA dose 289 mg/ kg per day) and OTA-melatonin-treated rats (melatonin dose 10 mg/kg per day). After four weeks of treatment, electrophoretic examinations were performed using SDSpolyacrylamide gel electrophoresis and Western blotting of hippocampal homogenates of the different groups. The concentrations of NR2A and NR2B in the OTA group were significantly lower than in the control group. The concentration of NR2B was significantly increased when melatonin was co-administered with OTA compared with OTA only. There was also a significant increase in NR2A levels when melatonin was co-administered with OTA. As a result, subchronic administration of OTA reduced hippocampal NMDA receptor subunits 2A and 2B concentrations in rats. It was thought that this alteration might affect cognitive functions because hippocampal NMDA receptors are involved in the memory and learning processes. Melatonin exhibited a partially protective effect on NR2A and NR2B against OTA.

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