Assessment of Cytokine-Modulated Proteasome Activity

Author(s):  
Christopher J. Kirk ◽  
Saul R. Powell ◽  
Edmund J. Miller
Keyword(s):  
2020 ◽  
Vol 01 ◽  
Author(s):  
Ayşe Mine Yılmaz ◽  
Gökhan Biçim ◽  
Kübra Toprak ◽  
Betül Karademir Yılmaz ◽  
Irina Milisav ◽  
...  

Background: Different cellular responses influence the progress of cancer. In this study, we have investigated the effect of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis and oxidative stress in human hepatocellular carcinoma (HepG2) cells. Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50 and proteasome activity were determined by Western blot and fluorometry, respectively. Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment. Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.


The Analyst ◽  
2021 ◽  
Author(s):  
Madalina M. Barsan ◽  
Victor C. Diculescu

The 20S proteasome is immobilized through specific interactions with antibodies and its activity is evaluated by electrochemical methods.


2012 ◽  
Vol 417 (1) ◽  
pp. 262-267 ◽  
Author(s):  
Paul G. Thomes ◽  
Casey S. Trambly ◽  
Geoffrey M. Thiele ◽  
Michael J. Duryee ◽  
Howard S. Fox ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77791 ◽  
Author(s):  
Mark Rinnerthaler ◽  
Renata Lejskova ◽  
Tomas Grousl ◽  
Vendula Stradalova ◽  
Gino Heeren ◽  
...  

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