Surgical Access to Cisterna Magna Using Concorde-Like Position for Cell Transplantation in Mice and CNS Dissection within Intact Dura for Evaluation of Cell Distribution

2016 ◽  
pp. 141-149
Author(s):  
Miroslaw Janowski
Keyword(s):  
Cell Transplantation ◽  
Cell Distribution ◽  
Cisterna Magna ◽  
Surgical Access

scholarly journals Intra-arterial Cell Transplantation Provides Timing-Dependent Cell Distribution and Functional Recovery After Stroke

Stroke ◽  
2013 ◽  
Vol 44 (3) ◽  
pp. 720-726 ◽  
Author(s):  
Shunsuke Ishizaka ◽  
Nobutaka Horie ◽  
Katsuya Satoh ◽  
Yuhtaka Fukuda ◽  
Noriyuki Nishida ◽  
...  
Keyword(s):  
Cell Transplantation ◽  
Functional Recovery ◽  
Cell Distribution ◽  
Dependent Cell

scholarly journals Elevated Red Blood Cell Distribution Width as a Poor Prognostic Factor in Patients With Hematopoietic Stem Cell Transplantation

2021 ◽  
Vol 10 ◽  
Author(s):  
Xiaojiong Jia ◽  
Si Cheng ◽  
Long Zhang ◽  
Yuan Zheng ◽  
Hua Zou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Prognostic Factor ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Cell Distribution ◽  
Distribution Width ◽  
Hematopoietic Stem ◽  
Kaplan Meier

Red cell distribution width (RDW), a measure of erythrocyte size variability, has been recently reported as an effective prognostic factor in critical illness. Hematopoietic stem cell transplantation (HSCT) has become the first choice of most patients with hematological malignancies. The aim of this study was to assess the changes of RDW in patients with HSCT and analyze the relationship between RDW and HSCT. In this study, we retrospectively enrolled 114 hematopoietic stem cell transplant patients during the period from 2015 to 2019. Logistic regression and Kaplan–Meier survival analysis were used for retrospective analysis. Multivariate analysis suggested that patients with elevated RDW (>14.5%) at three months post-transplantation have a poor clinical outcome compared with those with normal RDW ≤14.5% [odds ratio (OR) 5.12; P = 0.002]. Kaplan–Meier method analysis demonstrated that patients with elevated RDW levels (>14.5%) after hematopoietic stem cell transplantation experienced shorter progression-free survival compared to those with normal RDW levels (P = 0.008). Our study demonstrated that RDW could be an easily available and potential predictive biomarker for risk stratification in patients with HSCT. Further prospective studies are determined to confirm the prognostic value of RDW in HSCT patients.

scholarly journals Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Toshio Miki ◽  
Chika Takano ◽  
Irving M. Garcia ◽  
Brendan H. Grubbs
Keyword(s):  
Cell Transplantation ◽  
Cell Tracking ◽  
Therapeutic Option ◽  
Venous System ◽  
Cell Distribution ◽  
Portal Venous System ◽  
Injection Port ◽  
System A ◽  
Surgical Model ◽  
Multiple Cell

The liver is the largest internal organ and the center of homeostatic metabolism. Liver-directed cell transplantation is, therefore, an attractive therapeutic option to treat various metabolic disorders as well as liver diseases. Although clinical liver-directed cell transplantation requires multiple cell injections into the portal venous system, a mouse model is lacking which allows us to perform repetitive cell injections into the portal venous system. Here, we propose a surgical model that utilizes the spleen as a subcutaneous injection port. Mouse spleens were translocated under the skin with intact vascular pedicles. Human placental stem cell transplantations were performed one week following this port construction and repeated three times. Cell distribution was analyzed by quantifying human DNA using human Alu-specific primers. About 50% of the transplanted cells were located homogeneously in the liver one hour after the splenic port injection. Fluorescent-labeled cell tracking and antihuman mitochondrion immunohistochemistry studies demonstrated that the cells localized predominantly in small distal portal branches. A similar cell distribution was observed after multiple cell injections. These data confirm that the subcutaneous splenic injection port is suitable for performing repetitive cell transplantation into the portal venous system of mouse models.

Abstract 97: Intra-Arterial Cell Transplantation Provides Timing-Dependent Cell Distribution and Functional Recovery After Stroke

Stroke ◽  
2013 ◽  
Vol 44 (suppl_1) ◽  
Author(s):  
Shunsuke Ishizaka ◽  
Nobutaka Horie ◽  
Yutaka Fukuda ◽  
Katsuya Satoh ◽  
Noriyuki Nishida ◽  
...  
Keyword(s):  
Cell Transplantation ◽  
Functional Recovery ◽  
Human Mesenchymal Stem Cells ◽  
Serum Levels ◽  
Artery Occlusion ◽  
Control Group ◽  
Cell Distribution ◽  
Post Stroke ◽  
Dependent Cell ◽  
Cerebral Artery Occlusion

Background and Purpose— Intra-arterial cell transplantation offers a novel therapeutic strategy for stroke; however, it remains unclear how the timing of cell administration affects cell distribution, brain repair processes and functional recovery. Here, we investigate the hypothesis that the timing of cell transplantation changes the behavior of the cell graft and the host environment in a way that affects functional recovery. Methods— Rats received human mesenchymal stem cells (hMSCs) via the internal carotid artery at 1, 4 or 7 days (D1, D4 or D7) after middle cerebral artery occlusion and reperfusion. Animals were sacrificed at various time points to assess cell distribution in correlation with the host cerebral hemodynamics, serum levels of matrix metallopeptidase-9 (MMP-9), infiltration of activated microglia, expression of brain derived neurotrophic factor (BDNF), angiogenesis, presence of reactive astrocytes, and neurological recovery. Results— hMSCs were widely distributed both in the periinfarct and core in D1, and dominantly in the periinfarct in D4, in parallel with the cerebral hemodynamic change. Very few cells were observed in D7. Only in D1 group, the serum level of MMP-9 is significantly lower than that in control group at 72 hours after cell transplantation. At day 7 post-stroke, activation of microglia was significantly suppressed both in the periinfarct and core in D1, and only in the periinfarct in D4. At day 21 post-stroke, BDNF was widely distributed throughout the periinfarct in D1 and D4, and angiogenesis was enhanced. Motor function improved earlier in D1 and later in D4, but only D1 exhibited reduced atrophy. Conclusions— Our results indicate that intra-arterial cell transplantation provides timing-dependent cell distribution and post-stroke functional recovery via a combination of two mechanisms: neuroprotection and neurorestoration.

630: A Novel Model System for the Treatment of Renal Cancer by Nonmyeloablative Allogeneic Hemopoietic Cell Transplantation Using Cyclophosphamide in Mice

2005 ◽  
Vol 173 (4S) ◽  
pp. 172-172
Author(s):  
Masatoshi Eto ◽  
Masahiko Harano ◽  
Katsunori Tatsugami ◽  
Hirofumi Koga ◽  
Seiji Naito
Keyword(s):  
Cell Transplantation ◽  
Renal Cancer ◽  
Model System ◽  
Hemopoietic Cell ◽  
Novel Model
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