scholarly journals Methods for In Vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in Tears, MTT, and TNF-α Release Assays

2016 ◽  
pp. 395-409
Author(s):  
Floriana Cappiello ◽  
Bruno Casciaro ◽  
Satya Sree Kolar ◽  
Hasna Baidouri ◽  
Alison M. McDermott ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Viability ◽  
Tnf Α ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ

2017 ◽  
Vol 8 ◽  
Author(s):  
Baiba Kurena ◽  
Elisabeth Müller ◽  
Panagiotis F. Christopoulos ◽  
Ingvild Bjellmo Johnsen ◽  
Branislava Stankovic ◽  
...  
Keyword(s):  
Semliki Forest Virus ◽  
Virus Vectors ◽  
Tnf Α ◽  
Ifn Γ ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA

2019 ◽  
Vol 12 (1) ◽  
pp. 200
Author(s):  
Vinoy Jacob ◽  
Rajiv P
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Pathogenic Bacteria ◽  
Curcuma Longa ◽  
Reducing Power ◽  
Zno Nps ◽  
Natural Medicine ◽  
Vitro Analysis ◽  
In Vitro Analysis

Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparticle (ZnO NPs) is famous nanoparticles which are economical, less toxic, and brilliantly biocompatible. They have potential biomedical properties, mainly anticancer, antidiabetic, and antimicrobial.Methods: The present study was designed to investigate in vitro analysis of the antimicrobial activity against pathogenic bacteria and fungi and its ability to scavenge reactive oxygen radicals of ZnO NPs.Results and Conclusion: The results indicated that ZnO NPs produced from C. longa had higher antimicrobial activity against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans. Therefore, we suggest that ZnO NPs can be used as the antimicrobial agent. It is a good scavenger of superoxide radical, nitric oxide, and hydrogen peroxide and has reducing power, which is greater than ascorbic acid at a higher concentration.

scholarly journals Crosstalk of Endothelial and Mesenchymal Stromal Cells under Tissue-Related O2

2021 ◽  
Vol 1 (2) ◽  
pp. 116-136
Author(s):  
Olga Zhidkova ◽  
Elena Andreeva ◽  
Mariia Ezdakova ◽  
Ludmila Buravkova
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reciprocal Effects ◽  
Hypoxic Stress ◽  
Protective Effects ◽  
Short Term ◽  
Tnf Α ◽  
Vitro Analysis ◽  
In Vitro Analysis

Mesenchymal stromal cells (MSCs) are considered a valuable tool for cell therapy. After systemic administration, the outcome of MSCs and endothelial cells (ECs) interactions strongly depend on the local microenvironment and tissue O2 levels in particular. In vitro analysis of EC effects on MSC regenerative potential in co-culture was performed after short-term interaction at “physiological” hypoxia (5% O2) and acute hypoxic stress (0.1% O2). At 5% O2, MSCs retained stromal phenotype and CFU-f numbers, osteogenic RUNX2 was upregulated. A shift in the expression of adhesion molecules, and an increase in transcription/synthesis of IL-6, IL-8 contributed to facilitation of directed migration of MSCs. In the presence of MSCs, manifestations of oxidative stress in ECs were attenuated, and a decrease in adhesion of PBMCs to TNF-α-activated ECs was observed. Under 0.1% O2, reciprocal effects of ECs and MSCs were similar to those at 5% O2. Meanwhile, upregulation of RUNX2 was canceled, IL-6 decreased, and IL-8 significantly increased. “Protective” effects of MSCs on TNF-α-ECs were less pronounced, manifested as NOS3 downregulation and intracellular NO elevation. Therefore, interaction with ECs at “physiological” hypoxia enhanced pro-regenerative capacities of MSCs including migration and anti-inflammatory modulation of ECs. Under acute hypoxic stress, the stimulating effects of ECs on MSCs and the “protective” potential of MSCs towards TNF-α-ECs were attenuated.

Preparation and In Vitro Analysis of Microcapsule Thalidomide Formulation for Targeted Suppression of TNF-α

Drug Delivery ◽  
2006 ◽  
Vol 13 (5) ◽  
pp. 331-337 ◽  
Author(s):  
Terrence Metz ◽  
Tasima Haque ◽  
Hongmei Chen ◽  
Satya Prakash ◽  
Devendra Amre ◽  
...  
Keyword(s):  
Tnf Α ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA

2019 ◽  
Vol 12 (1) ◽  
pp. 200 ◽  
Author(s):  
Vinoy Jacob ◽  
Rajiv P
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Pathogenic Bacteria ◽  
Curcuma Longa ◽  
Reducing Power ◽  
Zno Nps ◽  
Natural Medicine ◽  
Vitro Analysis ◽  
In Vitro Analysis

Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparticle (ZnO NPs) is famous nanoparticles which are economical, less toxic, and brilliantly biocompatible. They have potential biomedical properties, mainly anticancer, antidiabetic, and antimicrobial.Methods: The present study was designed to investigate in vitro analysis of the antimicrobial activity against pathogenic bacteria and fungi and its ability to scavenge reactive oxygen radicals of ZnO NPs.Results and Conclusion: The results indicated that ZnO NPs produced from C. longa had higher antimicrobial activity against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans. Therefore, we suggest that ZnO NPs can be used as the antimicrobial agent. It is a good scavenger of superoxide radical, nitric oxide, and hydrogen peroxide and has reducing power, which is greater than ascorbic acid at a higher concentration.

Aqueous Humor Antimicrobial Activity: In Vitro Analysis after Topical 0.5% Chloramphenicol Application

2017 ◽  
Vol 42 (6) ◽  
pp. 847-851 ◽  
Author(s):  
Carlo Cagini ◽  
Annalisa Dragoni ◽  
Giampaolo Orsolini ◽  
Tito Fiore ◽  
Alfredo Beccasio ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Humor ◽  
Vitro Analysis ◽  
In Vitro Analysis
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