Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs

2017 ◽  
pp. 369-380 ◽  
Author(s):  
Cristina Colosi ◽  
Marco Costantini ◽  
Andrea Barbetta ◽  
Mariella Dentini
Keyword(s):  
Tissue Constructs

scholarly journals Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 110 ◽  
Author(s):  
Erika Ferrari ◽  
Cecilia Palma ◽  
Simone Vesentini ◽  
Paola Occhetta ◽  
Marco Rasponi
Keyword(s):  
Imaging Techniques ◽  
Biological Models ◽  
Electromechanical Properties ◽  
Human Organs ◽  
Tissue Constructs ◽  
Microphysiological Systems ◽  
Metabolic Activities ◽  
On Chip ◽  
Chip Analysis

Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

scholarly journals Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 915
Author(s):  
Gözde Dursun ◽  
Muhammad Umer ◽  
Bernd Markert ◽  
Marcus Stoffel
Keyword(s):  
Mobile Application ◽  
Low Cost ◽  
Experimental Information ◽  
Raspberry Pi ◽  
Tissue Constructs ◽  
Cost Controlling ◽  
Controlling System ◽  
And Control ◽  
Tissue Models

(1) Background: Bioreactors mimic the natural environment of cells and tissues by providing a controlled micro-environment. However, their design is often expensive and complex. Herein, we have introduced the development of a low-cost compression bioreactor which enables the application of different mechanical stimulation regimes to in vitro tissue models and provides the information of applied stress and strain in real-time. (2) Methods: The compression bioreactor is designed using a mini-computer called Raspberry Pi, which is programmed to apply compressive deformation at various strains and frequencies, as well as to measure the force applied to the tissue constructs. Besides this, we have developed a mobile application connected to the bioreactor software to monitor, command, and control experiments via mobile devices. (3) Results: Cell viability results indicate that the newly designed compression bioreactor supports cell cultivation in a sterile environment without any contamination. The developed bioreactor software plots the experimental data of dynamic mechanical loading in a long-term manner, as well as stores them for further data processing. Following in vitro uniaxial compression conditioning of 3D in vitro cartilage models, chondrocyte cell migration was altered positively compared to static cultures. (4) Conclusion: The developed compression bioreactor can support the in vitro tissue model cultivation and monitor the experimental information with a low-cost controlling system and via mobile application. The highly customizable mold inside the cultivation chamber is a significant approach to solve the limited customization capability of the traditional bioreactors. Most importantly, the compression bioreactor prevents operator- and system-dependent variability between experiments by enabling a dynamic culture in a large volume for multiple numbers of in vitro tissue constructs.

scholarly journals Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (25) ◽  
pp. 5773-5781 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Quynhhoa T. Nguyen ◽  
Albert C. Chen ◽  
David L. Kaplan ◽  
Robert L. Sah ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Tissue Constructs

scholarly journals Hypoxia and Tension Maintain Human Tenocyte Tissue Constructs in the 3D Microenvironment

2017 ◽  
Vol 42 (9) ◽  
pp. S47
Author(s):  
Rowena McBeath ◽  
Richard W. Edwards ◽  
Robert L. Mauck ◽  
Irving Shapiro ◽  
A. Lee Osterman
Keyword(s):  
Tissue Constructs ◽  
3D Microenvironment

scholarly journals The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs

2010 ◽  
Vol 2010 (4) ◽  
pp. pdb.prot5416-pdb.prot5416 ◽  
Author(s):  
R. A. Poche ◽  
J. E. Saik ◽  
J. L. West ◽  
M. E. Dickinson
Keyword(s):  
Live Imaging ◽  
Tissue Constructs ◽  
Engineered Tissue ◽  
Transplantation Site

New directions in bioabsorbable technology

2002 ◽  
Vol 97 (4) ◽  
pp. 481-489 ◽  
Author(s):  
Stephen M. Warren ◽  
Marc H. Hedrick ◽  
Karl Sylvester ◽  
Michael T. Longaker ◽  
Constance M. Chen
Keyword(s):  
Interdisciplinary Approach ◽  
Patient Specific ◽  
Therapeutic Gene ◽  
Content Type ◽  
Tissue Constructs ◽  
New Directions ◽  
Material Sciences ◽  
Specific Tissue ◽  
Tissue Restoration ◽  
Fine Print

✓ Generating replacement tissues requires an interdisciplinary approach that combines developmental, cell, and molecular biology with biochemistry, immunology, engineering, medicine, and the material sciences. Because basic cues for tissue engineering may be derived from endogenous models, investigators are learning how to imitate nature. Endogenous models may provide the biological blueprints for tissue restoration, but there is still much to learn. Interdisciplinary barriers must be overcome to create composite, vascularized, patient-specific tissue constructs for replacement and repair. Although multistep, multicomponent tissue fabrication requires an amalgamation of ideas, the following review is limited to the new directions in bioabsorbable technology. The review highlights novel bioabsorbable design and therapeutic (gene, protein, and cell-based) strategies currently being developed to solve common spine-related problems.

Optically characterizing vascular tissue constructs made with soluble versus homogenized collagen

2008 ◽  
Author(s):  
David Levitz ◽  
Monica T. Hinds ◽  
Noi T. Tran ◽  
Stephen R. Hanson ◽  
Steven L. Jacques
Keyword(s):  
Vascular Tissue ◽  
Tissue Constructs

A rapid biofabrication technique for self-assembled collagen-based multicellular and heterogeneous 3D tissue constructs

2019 ◽  
Vol 92 ◽  
pp. 172-183 ◽  
Author(s):  
Alireza Shahin-Shamsabadi ◽  
P. Ravi Selvaganapathy
Keyword(s):  
Tissue Constructs ◽  
Self Assembled
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