scholarly journals Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 915
Author(s):  
Gözde Dursun ◽  
Muhammad Umer ◽  
Bernd Markert ◽  
Marcus Stoffel
Keyword(s):  
Mobile Application ◽  
Low Cost ◽  
Experimental Information ◽  
Raspberry Pi ◽  
Tissue Constructs ◽  
Cost Controlling ◽  
Controlling System ◽  
And Control ◽  
Tissue Models

(1) Background: Bioreactors mimic the natural environment of cells and tissues by providing a controlled micro-environment. However, their design is often expensive and complex. Herein, we have introduced the development of a low-cost compression bioreactor which enables the application of different mechanical stimulation regimes to in vitro tissue models and provides the information of applied stress and strain in real-time. (2) Methods: The compression bioreactor is designed using a mini-computer called Raspberry Pi, which is programmed to apply compressive deformation at various strains and frequencies, as well as to measure the force applied to the tissue constructs. Besides this, we have developed a mobile application connected to the bioreactor software to monitor, command, and control experiments via mobile devices. (3) Results: Cell viability results indicate that the newly designed compression bioreactor supports cell cultivation in a sterile environment without any contamination. The developed bioreactor software plots the experimental data of dynamic mechanical loading in a long-term manner, as well as stores them for further data processing. Following in vitro uniaxial compression conditioning of 3D in vitro cartilage models, chondrocyte cell migration was altered positively compared to static cultures. (4) Conclusion: The developed compression bioreactor can support the in vitro tissue model cultivation and monitor the experimental information with a low-cost controlling system and via mobile application. The highly customizable mold inside the cultivation chamber is a significant approach to solve the limited customization capability of the traditional bioreactors. Most importantly, the compression bioreactor prevents operator- and system-dependent variability between experiments by enabling a dynamic culture in a large volume for multiple numbers of in vitro tissue constructs.

scholarly journals Proanthocyanidins against Oxidative Stress: From Molecular Mechanisms to Clinical Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Lingyu Yang ◽  
Dehai Xian ◽  
Xia Xiong ◽  
Rui Lai ◽  
Jing Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Metabolic Diseases ◽  
Low Cost ◽  
Natural Agents ◽  
Molecular Damage ◽  
And Control

Proanthocyanidins (PCs) are naturally occurring polyphenolic compounds abundant in many vegetables, plant skins (rind/bark), seeds, flowers, fruits, and nuts. Numerousin vitroandin vivostudies have demonstrated myriad effects potentially beneficial to human health, such as antioxidation, anti-inflammation, immunomodulation, DNA repair, and antitumor activity. Accumulation of prooxidants such as reactive oxygen species (ROS) exceeding cellular antioxidant capacity results in oxidative stress (OS), which can damage macromolecules (DNA, lipids, and proteins), organelles (membranes and mitochondria), and whole tissues. OS is implicated in the pathogenesis and exacerbation of many cardiovascular, neurodegenerative, dermatological, and metabolic diseases, both through direct molecular damage and secondary activation of stress-associated signaling pathways. PCs are promising natural agents to safely prevent acute damage and control chronic diseases at relatively low cost. In this review, we summarize the molecules and signaling pathways involved in OS and the corresponding therapeutic mechanisms of PCs.

scholarly journals Ribosome Display Technology: Applications in Disease Diagnosis and Control

Antibodies ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 28
Author(s):  
Adinarayana Kunamneni ◽  
Christian Ogaugwu ◽  
Steven Bradfute ◽  
Ravi Durvasula
Keyword(s):  
In Vitro Selection ◽  
Low Cost ◽  
Disease Diagnosis ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Ribosome Display ◽  
Technology Applications ◽  
Display Technology ◽  
And Control

Antibody ribosome display remains one of the most successful in vitro selection technologies for antibodies fifteen years after it was developed. The unique possibility of direct generation of whole proteins, particularly single-chain antibody fragments (scFvs), has facilitated the establishment of this technology as one of the foremost antibody production methods. Ribosome display has become a vital tool for efficient and low-cost production of antibodies for diagnostics due to its advantageous ability to screen large libraries and generate binders of high affinity. The remarkable flexibility of this method enables its applicability to various platforms. This review focuses on the applications of ribosome display technology in biomedical and agricultural fields in the generation of recombinant scFvs for disease diagnostics and control.

scholarly journals High-availability on-site deployment to heterogeneous architectures for Project 8 and ADMX

2020 ◽  
Vol 245 ◽  
pp. 07029
Author(s):  
Benjamin LaRoque
Keyword(s):  
Coupling Parameter ◽  
Low Cost ◽  
Rapid Development ◽  
High Availability ◽  
Raspberry Pi ◽  
Development Environment ◽  
Heterogeneous Architectures ◽  
Active Research ◽  
Support Software ◽  
And Control

Project 8 is applying a novel spectroscopy technique to make a precision measurement of the tritium beta-decay spectrum, resulting in either a measurement of or further constraint on the effective mass of the electron antineutrino. ADMX is operating an axion haloscope to scan the mass-coupling parameter space in search of dark matter axions. Both collaborations are executing medium-scale experiments, where stable operations last for three to nine months and the same system is used for development and testing between periods of operation. It is also increasingly common to use low-cost computing elements, such as the Raspberry Pi, to integrate computing and control with custom instrumentation and hardware. This leads to situations where it is necessary to support software deployment to heterogeneous architectures on rapid development cycles while maintaining high availability. Here we present the use of docker containers to standardize packaging and execution of control software for both experiments and the use of kubernetes for management and monitoring of container deployment in an active research and development environment. We also discuss the advantages over more traditional approaches employed by experiments at this scale, such as detached user execution or custom control shell scripts.

scholarly journals Control System for a Tensile-Testing Device Using Low-Cost Hardware and Open-Source Software

2020 ◽  
Vol 66 (3) ◽  
pp. 155-163 ◽  
Author(s):  
Matej Kranjec ◽  
Jernej Korinšek ◽  
Miha Ambrož ◽  
Robert Kunc
Keyword(s):  
Control System ◽  
Tensile Testing ◽  
Soft Tissues ◽  
Low Cost ◽  
Raspberry Pi ◽  
Material Research ◽  
Testing Device ◽  
Controlling System ◽  
Comparison Of The Results

The aim of this study is to verify whether a Raspberry Pi 3 B+ can be utilized as a low-cost device for controlling a tensile-testing device used for material research purposes. A list of requirements based on already-available hardware was drawn up, which the new control system had to fulfil. To connect all the necessary equipment, a connection board was constructed, and some additional hardware was acquired for the system to be able to perform all the necessary tasks. The whole controlling system was also put in a small enclosure to make it portable. The control-system software was written in C++ using the Pigpio library. The developed system was then tested, and the results were compared to a commercially available Instron 8802 device. A comparison of the results shows that the upgraded equipment can produce comparable results to commercially available devices and is sufficiently accurate to be applied for research purposes for the characterization of soft tissues and other materials.

scholarly journals Implementation of vehicle ventilation system using NodeMCU ESP8266 for remote monitoring

2021 ◽  
Vol 10 (1) ◽  
pp. 327-336
Author(s):  
Amirun Murtaza Abd Jalil ◽  
Roslina Mohamad ◽  
Nuzli Mohamad Anas ◽  
Murizah Kassim ◽  
Saiful Izwan Suliman
Keyword(s):  
Mobile Applications ◽  
Mobile Application ◽  
Transmission Control Protocol ◽  
Low Cost ◽  
Ventilation System ◽  
Time Data ◽  
Transmission Control ◽  
Iot Platform ◽  
Before And After ◽  
And Control

In this paper, an implementation of vehicle ventilation system using microcontroller NodeMCU is described, as an internet of things (IoT) platform. A low-cost wireless fidelity (Wi-Fi) microchip ESP8266 integrated with NodeMCU provides full-stack transmission control protocol/internet protocol (TCP/IP) to communicate between mobile applications. This chip is capable to monitor and control sensor devices connected to the IoT platform. In this reserach, data was collected from a temperature sensor integrated to the platform, which then monitored using Blynk application. The vehicle ventilation system was activated/deactivated through mobile application and controlled using ON/OFF commands sent to the connected devices. From the results, the vehicle ventilation system built using NodeMCU microcontroller is capable to provide near real-time data monitoring for temperature in the car before and after the ventilation system was applied.

scholarly journals Water Consumption and Water Leakage Alert Using IoT

2019 ◽  
pp. 647-650
Author(s):  
Anand. P ◽  
Devipreetha. K ◽  
Haripriya. R
Keyword(s):  
Water Supply ◽  
Water Flow ◽  
Flow Rate ◽  
Water Consumption ◽  
Mobile Application ◽  
Water Flow Rate ◽  
Flow Monitoring ◽  
Controlling System ◽  
Current Water ◽  
And Control

Managing water consumption is important for life preservation. Knowing water consumption at homes can have a great impact on water saving. There is a global water crisis due to increasing population growth, climate change, increasing consumption. Giving a report about the state of the planet’s water, especially in developing countries, the report describes the outlook for future generations as worries. To visually check water taps in the house consumes time and requires a family member to be at the house. To remotely do so, we propose a system that monitors, alerts the user and allows the user to control the water flow through taps whenever there is an unusual reading of the water usage at home. The Water Flow Monitoring and Controlling System is an android- based mobile application. It is equipped with external hardware to sense a tap’s water flow rate and control which means turning on or off the water supply line whenever necessary. Registered users can login and view their house’s current water flowage from the mobile application. The external hardware updates the water flow rate at every specified time to a database through the Internet connection. If the users decide to turn on or off the water supply taps at their homes, it can be done through the on or off button provided in the mobile application. A user’s on or off instruction is set within the database. The hardware receives this instruction and performs the desired action.

Integrated Wireless Monitoring System Using LoRa and Node-Red for University Building

2019 ◽  
Vol 16 (8) ◽  
pp. 3384-3394
Author(s):  
Sathish Kumar Selvaperumal ◽  
Waleed Al-Gumaei ◽  
Raed Abdulla ◽  
Vinesh Thiruchelvam
Keyword(s):  
User Interface ◽  
Control Systems ◽  
Graphical User Interface ◽  
Mobile Application ◽  
Raspberry Pi ◽  
Time Data ◽  
Wireless Monitoring ◽  
Smart Campus ◽  
And Control ◽  
The Internet Of Things

This paper aims to design and develop a network infrastructure for a smart campus using the Internet of Things which can be used to control different devices and to update the management with real-time data. In this proposed system, NodeMCU ESP8266 is interfaced with thermal and motion sensor for human, humidity and temperature sensor for the room and relay to control the lights and the air-conditioned. MQTT broker is used to acquire the data and control to and from NodeMCU ESP8266, Raspberry pi and LoRa, to be interfaced wirelessly with the Node-Red. Thus, the system is controlled and monitored wirelessly with the help of the developed integrated Graphical User Interface along with the Mobile application. The performance of the developed proposed system is analyzed and evaluated by testing the motion detection in the classroom, the LoRa range with the RSSI, the average time taken by the system to respond, the average time taken for the Graphical User Interface to response and update its data. Finally, the average time taken by the system and the Graphical User Interface to respond to the lights and air-conditioned control systems is less than 1 s, and for the security and parking systems is less than 2 s.

scholarly journals Probiotic Potential of Blueberry Jam Fermented with Lactic Acid Bacteria

2020 ◽  
Vol 8 (1) ◽  
pp. 65-78
Author(s):  
Aditya Chaudhary ◽  
Khushbu Verma ◽  
Baljeet Singh Saharan
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Low Cost ◽  
Antioxidant Properties ◽  
Titratable Acidity ◽  
Food Product ◽  
Good Health ◽  
Probiotic Strains ◽  
And Control

The present study assesses the feasibility of blueberry as a raw substrate for the production of the probiotic blueberry jam by lactic acid bacteria (L. plantarum DB-2, L. fermentum J-1, P. acidilactici M-3, L. plantarum SK-3, and P. pentosaceus SM-2). Changes in pH, titratable acidity (lactic acid), cell survival, antioxidant properties, and in vitro cholesterol reduction properties of lacto- fermented as well as non-fermented blueberry jam were examined during fermentation and up to 28 days of storage. All the strains grew well in a lacto-fermented blueberry jam after 48 h fermentation. Set A (5.88 g/100 ml) and Set B (5.96 g/100 ml) produced less lactic acid than Set C (6.67 g/100 ml) which has the consortia of probiotic strains. After 28 days of cold storage, all the tested strains survived the low-pH conditions in lacto-fermented blueberry jam. The blueberry jam fermented with the consortia of probiotic strains (Set C) had a high antioxidant capacity (71.47 ± 3.57) in comparison with Set A, Set B, and control which showed anti-oxidant capacity viz. 70.52 ± 3.52, 70.25 ± 3.18, and 64.12 ± 2.47, respectively after 28 days of refrigerated storage. The lacto- fermented blueberry jam in Set C (58.48%) had shown the in vitro cholesterol-lowering ability better than Set B (18.87%) whereas Set A and control did not show any in vitro reduction in cholesterol level after 28 days of storage. Sensory quality studies were carried out after 28 days of storage. Sensory evaluation data showed the considerable acceptability of the lacto-fermented blueberry jam. Finally, we found that L. plantarum DB-2, L. fermentum J-1, P. acidilactici M-3, L. plantarum SK-3, and P. pentosaceus SM-2 are optimal probiotics for fermentation with blueberry jam. In this investigation, the results could be an indicator of the development of health-promoting fruit jam. This lacto-fermented blueberry jam is a low-cost healthy food product, provide better nutrition and good health to the population.

Low Cost Real Time Water effluence Monitoring and Controlling System using IOT Module

2020 ◽  
Vol 52 (3) ◽  
pp. 05-07
Author(s):  
Nicolette Léveillé
Keyword(s):  
Drinking Water ◽  
Water Body ◽  
Water Contamination ◽  
Low Cost ◽  
Raspberry Pi ◽  
Safe Drinking Water ◽  
Controlling System ◽  
Set Up ◽  
Minimal Effort ◽  
Traditional Strategy

Water is an essential component required for people and thusly there must be instruments set up to overwhelmingly test the nature of savouring water ongoing. The traditional strategy for taxing water excellence is to accumulate tests of water physically and investigate. This strategy is tedious, surplus of labour, and not conservative. This paper proposes a minimal effort framework for constant water contamination observing and domineering utilizing IoT. The Zig-Bee module in the framework moves information gathered by the sensors to the micro-controller remotely, and GSM based module moves remotely the information MC to PC. The sensor esteems prepared by Raspberry pi and send to the cloud. The framework likewise has nearness sensors to make the authorities by communicating something specific aware of them through the GSM module in the event that somebody attempts to contaminate the water body. This framework can keep an exacting mind the contamination of the water assets and have the option to give a situation to safe drinking water.

scholarly journals An Approach to Industrial Automation Based on Low-Cost Embedded Platforms and Open Software

2020 ◽  
Vol 10 (14) ◽  
pp. 4696 ◽  
Author(s):  
Luis I. Minchala ◽  
Jonnathan Peralta ◽  
Paul Mata-Quevedo ◽  
Jaime Rojas
Keyword(s):  
Fault Detection ◽  
Open Source ◽  
Low Cost ◽  
Fault Detection And Diagnosis ◽  
Raspberry Pi ◽  
Enabling Factors ◽  
Pid Algorithm ◽  
Embedded Platforms ◽  
Detection And Diagnosis ◽  
And Control

This paper presents a performance evaluation of the development of the instrumentation, communications and control systems of a two-tank process by using low-cost hardware and open source software. The hardware used for automating this process consists of embedded platforms (Arduino and Raspberry Pi) integrated into programmable logic controllers (PLCs), which are connected to a supervisory control and data acquisition (SCADA) system implemented with an open source Industrial Internet of Things (IIoT) platform. The main purpose of the proposed approach is to evaluate low-cost automation solutions (hardware and software) within the framework of modern industry requirements in order to determine whether these technologies could be enabling factors of IIoT. The proposed control strategy for regulating tank levels combines the classic PID algorithm and the fuzzy gain scheduling PID (FGS-PID) approach. Fault detection capabilities are also enabled for the system through a fault detection and diagnosis module (FDD) implemented with an extended Kalman filter (EKF). The distributed controller’s (DC) algorithms are embedded into the PLC’s processors in order to demonstrate the flexibility of the proposed system. Additionally, a remote human to machine interface (HMI) is deployed through a web client of the IIoT application. Experimental results show the proper operation of the overall system.

Sign in / Sign up

Export Citation Format

Share Document