High-Resolution Parallel Reaction Monitoring with Electron Transfer Dissociation for Middle-Down Proteomics: An Application to Study the Quantitative Changes Induced by Histone Modifying Enzyme Inhibitors and Activators

2017 ◽  
pp. 61-69 ◽  
Author(s):  
Michael J. Sweredoski ◽  
Annie Moradian ◽  
Sonja Hess
Keyword(s):  
Electron Transfer ◽  
High Resolution ◽  
Electron Transfer Dissociation ◽  
Enzyme Inhibitors ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Parallel Reaction Monitoring ◽  
Quantitative Changes

High Resolution Parallel Reaction Monitoring with Electron Transfer Dissociation for Middle-Down Proteomics

2015 ◽  
Vol 87 (16) ◽  
pp. 8360-8366 ◽  
Author(s):  
Michael J. Sweredoski ◽  
Annie Moradian ◽  
Matthias Raedle ◽  
Catarina Franco ◽  
Sonja Hess
Keyword(s):  
Electron Transfer ◽  
High Resolution ◽  
Electron Transfer Dissociation ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Parallel Reaction Monitoring

scholarly journals Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring

2016 ◽  
Vol 57 (4) ◽  
pp. 714-728 ◽  
Author(s):  
Sasha A. Singh ◽  
Allison B. Andraski ◽  
Brett Pieper ◽  
Wilson Goh ◽  
Carlos O. Mendivil ◽  
...  
Keyword(s):  
High Resolution ◽  
Accurate Mass ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Parallel Reaction Monitoring ◽  
High Resolution Accurate Mass

scholarly journals The Development of a Liquid Chromatography High-Resolution Mass Spectrometric Method for Apixaban Quantification in Dried Plasma Spots in Parallel Reaction Monitoring Mode

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 450
Author(s):  
Alexander Chernonosov ◽  
Liliya Aksenova ◽  
Vladimir Koval
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Method Development ◽  
Extraction Procedure ◽  
Mass Spectrometric Method ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
High Resolution Mass ◽  
Parallel Reaction Monitoring ◽  
Resolution Mass

This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high-resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 µL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 µL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 µL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra- and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room temperature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust.

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