Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells

2018 ◽  
pp. 137-144 ◽  
Author(s):  
Eugenio Martignani ◽  
Paolo Accornero ◽  
Silvia Miretti ◽  
Mario Baratta
Keyword(s):  
Mammary Cells

GENE ACTIVATION IN MAMMARY CELLS

1972 ◽  
Vol 71 (2_Suppla) ◽  
pp. S346-S368 ◽  
Author(s):  
Roger W. Turkington ◽  
Nobuyuki Kadohama
Keyword(s):  
Cell Differentiation ◽  
Gene Transcription ◽  
Hormonal Regulation ◽  
Gene Activation ◽  
Milk Proteins ◽  
Mouse Mammary Gland ◽  
Nuclear Proteins ◽  
Mammary Cells ◽  
Alveolar Cells ◽  
Mammary Cell

ABSTRACT Hormonal activation of gene transcription has been studied in a model system, the mouse mammary gland in organ culture. Transcriptive activity is stimulated in mammary stem cells by insulin, and in mammary alveolar cells by prolactin and insulin. Studies on the template requirement for expression of the genes for milk proteins demonstrate that DNA methylation has an obligatory dependence upon DNA synthesis, but is otherwise independent from hormonal regulation of mammary cell differentiation. Incorporation of 5-bromo-2′deoxyuridine into DNA selectively inhibits expression of the genes for specific milk proteins. Undifferentiated mammary cells activate the synthesis of specific acidic nuclear proteins when stimulated by insulin. Several of these induced acidic nuclear proteins are undetectable in unstimulated undifferentiated cells, but appear to be characteristic components of the nuclei of differentiated cells. These results indicate that mammary cell differentiation is associated with a change in acidic nuclear proteins, and they provide evidence to support the concept that acidic nuclear proteins may be involved in the regulation of gene transcription and of mammary cell differentiation.

Investigation of the role of microtubules in protein secretion from lactating mouse mammary epithelial cells

1992 ◽  
Vol 102 (2) ◽  
pp. 239-247 ◽  
Author(s):  
M.E. Rennison ◽  
S.E. Handel ◽  
C.J. Wilde ◽  
R.D. Burgoyne
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Protein Secretion ◽  
Secretory Pathway ◽  
Mammary Epithelial Cells ◽  
Early Stage ◽  
Major Effect ◽  
Vesicle Transport ◽  
Mammary Epithelial ◽  
Mammary Cells

Disruption of microtubules has been shown to reduce protein secretion from lactating mammary epithelial cells. To investigate the involvement of microtubules in the secretory pathway in these cells we have examined the effect of nocodazole on protein secretion from mammary epithelial cells derived from the lactating mouse. Mouse mammary cells have extensive microtubule networks and 85% of their tubulin was in a polymeric form. Treatment with 1 micrograms/ml nocodazole converted most of the tubulin into a soluble form. In a continuous labelling protocol it was found that nocodazole did not interfere with protein synthesis but over a 5 h period secretion was markedly inhibited. To determine whether the inhibition was at the level of early or late stages of the secretory pathway mammary cells were pulse-labelled for 1 h to label protein throughout the secretory pathway before nocodazole treatment. When secretion was subsequently assayed it was found to be slower and only partially inhibited. These findings suggest that the major effect of nocodazole is on an early stage of the secretory pathway and that microtubules normally facilitate vesicle transport to the plasma membrane. An involvement of microtubules in vesicle transport to the plasma membrane is consistent with an observed accumulation of casein vesicles in nocodazole-treated cells. Exocytosis stimulated by the calcium ionophore ionomycin was unaffected by nocodazole treatment. We conclude from these results that the major effect of nocodazole is at an early stage of the secretory pathway, one possible target being casein vesicle biogenesis in the trans-Golgi network.

In vitro model of parathyroid hormone-related protein secretion from mammary cells isolated from lactating cows

1996 ◽  
Vol 13 (5) ◽  
pp. 399-410 ◽  
Author(s):  
H. Okada ◽  
F.L. Schanbacher ◽  
L.K. McCauley ◽  
M.T. Weckmann ◽  
C.C. Capen ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Protein Secretion ◽  
In Vitro Model ◽  
Mammary Cells ◽  
Related Protein ◽  
Lactating Cows ◽  
Parathyroid Hormone Related Protein ◽  
Vitro Model

Morphological and functional differentiation of cryopreserved lactating bovine mammary cells cultured on floating collagen gels

1993 ◽  
Vol 25 (6) ◽  
pp. 799-816 ◽  
Author(s):  
Rabih S. Talhouk ◽  
Ronald L. Neiswander ◽  
Floyd L. Schanbacher
Keyword(s):  
Functional Differentiation ◽  
Mammary Cells ◽  
Collagen Gels ◽  
Cells Cultured

Lactoferrin interaction with retinoid signaling: Cell growth and apoptosis in mammary cells

2006 ◽  
Vol 30 (4) ◽  
pp. 289-303 ◽  
Author(s):  
Craig R. Baumrucker ◽  
Floyd Schanbacher ◽  
Youngfeng Shang ◽  
M.H. Green
Keyword(s):  
Cell Growth ◽  
Mammary Cells ◽  
Retinoid Signaling

Oscillating activity of a calcium-activated K+ channel in normal and cancerous mammary cells in culture

1991 ◽  
Vol 119 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Koh-Ichi Enomoto ◽  
Kishio Furuya ◽  
Takashi Maeno ◽  
Charles Edwards ◽  
Takami Oka
Keyword(s):  
K Channel ◽  
Mammary Cells ◽  
Cells In Culture
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